Team:British Columbia/Wetlabteam

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Team: British Columbia - 2011.igem.org

Team Members

Gurpal Bisra: My contribution to our wet lab component was to alter a yeast cell to contain the mutant erg20-2 gene instead of the wild type erg20 gene. Erg20 produces ~25% GPP and ~75% FPP molecules in the mevalonate biochemical pathway. GPP is essential to our project because it is the molecule that combines with a synthase in order to yield a monoterpene; where as, FPP does not develop monoterpenes. Since our goal is to mass produce monoterpenes, this mutation is key for our project to be successful. Additionally, I am assisting Samuel Wu to develop plasmids that contain the K6R-HMG2 and IDI1 genes. Their function is to alter the mevalonate pathway to ultimately produce more GPP as well.	Daisy Ji	British Columbia Team member 3.png Marianne	British Columbia Team member 4.png Vicki
British Columbia Team member 5.png Joe: I am a 4th year science student at UBC, studying microbiology and genetics.	British Columbia Team member 6.png Sam	British Columbia Team member 6.png Jacob

Graduate Advisors

Alina Chan: My main wet lab role is to train team members in the art of working with yeast. I also help with general troubleshooting and experimental design.

Rafael, the fat monkey: oooooooooaaaaahhhhh

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 ==Team Members==
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-Image:Gurpal.jpg|Gurpal Bisra: I am an engineering physicist student interested in bioengineering and biology in general. I have traveled to 14 countries so far and my thirst for adventure never ceases. In my spare time, I enjoy drawing, doing martial arts, making people laugh and playing badminton.
+Image:Gurpal.jpg|Gurpal Bisra: My contribution to our wet lab component was to alter a yeast cell to contain the mutant erg20-2 gene instead of the wild type erg20 gene. Erg20 produces ~25% GPP and ~75% FPP molecules in the mevalonate biochemical pathway. GPP is essential to our project because it is the molecule that combines with a synthase in order to yield a monoterpene; where as, FPP does not develop monoterpenes. Since our goal is to mass produce monoterpenes, this mutation is key for our project to be successful. Additionally, I am assisting Samuel Wu to develop plasmids that contain the K6R-HMG2 and IDI1 genes. Their function is to alter the mevalonate pathway to ultimately produce more GPP as well.
-My contribution to our wet lab component was to alter a yeast cell to contain the mutant erg20-2 gene instead of the wild type erg20 gene. Erg20 produces ~25% GPP and ~75% FPP molecules in the mevalonate biochemical pathway. GPP is essential to our project because it is the molecule that combines with a synthase in order to yield a monoterpene; where as, FPP does not develop monoterpenes. Since our goal is to mass produce monoterpenes, this mutation is key for our project to be successful.
-Additionally, I am assisting Samuel Wu to develop plasmids that contain the K6R-HMG2 and IDI1 genes. Their function is to alter the mevalonate pathway to ultimately produce more GPP as well.
 Image:Daisy.jpg|Daisy Ji