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Team:British Columbia/Notebook/12 July 2011
From 2011.igem.org
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| - | *After 5 SDMs, transformations, and minipreps, Jacob should now have ECORI-less, SPEI-less, XBAI-less, PSTI-less pg-tps-cin and pgxe-tps-cin. Sequencing is the next step to confirm this result. | + | *After 5 SDMs, transformations, and minipreps, Jacob should now have ECORI-less, SPEI-less, XBAI-less, PSTI-less pg-tps-cin and pgxe-tps-cin. The gel of the restriction digest has the correct number of bands. Sequencing is the next step to confirm this result. |
Latest revision as of 22:08, 1 August 2011
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July 12 2011
beta-pinene
- Miniprep one colony from each of the three plates.
(-)-limonene
- Gel verification shows no bands
- Troubleshooting: Use a different cycling condition
1,8-cineole
- After 5 SDMs, transformations, and minipreps, Jacob should now have ECORI-less, SPEI-less, XBAI-less, PSTI-less pg-tps-cin and pgxe-tps-cin. The gel of the restriction digest has the correct number of bands. Sequencing is the next step to confirm this result.
