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Team:British Columbia/Notebook/18 July 2011
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*sequencing failed for all of P2SDM1, P2SDM2, P2SDM3. | *sequencing failed for all of P2SDM1, P2SDM2, P2SDM3. | ||
*restriction digested the miniprepped plasmids to see if the plasmids get cut (if the site is still there, then the vector should cut and give a single band). | *restriction digested the miniprepped plasmids to see if the plasmids get cut (if the site is still there, then the vector should cut and give a single band). | ||
*gel verification of the digests suggest that the site has been removed (show bands that are identical to the uncut template/original vector). | *gel verification of the digests suggest that the site has been removed (show bands that are identical to the uncut template/original vector). | ||
Latest revision as of 23:54, 20 July 2011
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July 18 2011
beta-pinene
- sequencing failed for all of P2SDM1, P2SDM2, P2SDM3.
- restriction digested the miniprepped plasmids to see if the plasmids get cut (if the site is still there, then the vector should cut and give a single band).
- gel verification of the digests suggest that the site has been removed (show bands that are identical to the uncut template/original vector).
