Team:British Columbia/Accomplishments

From 2011.igem.org

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<center><h2 style="margin:12px;font-weight:bold"> Project Accomplishments
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<h2 style="margin:12px;font-weight:bold"> Wet Laboratory Achievements
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===Wet Laboratory Results===
 
<h3>1. In vitro assay production of monoterpene in bacteria</h3>
<h3>1. In vitro assay production of monoterpene in bacteria</h3>
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===Modeling Results===
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<h2 style="margin:12px;font-weight:bold"> Modeling Achievements
'''Monoterpene Production'''  
'''Monoterpene Production'''  

Revision as of 00:19, 28 October 2011

Team: British Columbia - 2011.igem.org

Wet Laboratory Achievements

Contents

1. In vitro assay production of monoterpene in bacteria

Geranyl Pyrophosphate (GPP) Assay


Alpha-beta pinene and Beta-pinene synthases were The purified enzymes were assayed in vitro with GPP and sent for GC-MS, which confirmed alpha- and beta-pinene as products.


Ubcigem2011betapingcms.jpg


2. Diterpene production in yeast

3. Mountain Pine Beetle & Yeast Co-culture

2. Blue Stain Fungi & Yeast Co-culture

<h2 style="margin:12px;font-weight:bold"> Modeling Achievements

Monoterpene Production


Monoterpene Production vs Time in Pinene Synthase.jpg


We simulated the expansion of the MPB population from year 2011 to 2020 using the estimates obtained from the clustering analysis. For cost estimation and prediction of emergence of subpopulations, refer to our Model Methodology above.