Team:SJTU-BioX-Shanghai/Parts/Data Page

From 2011.igem.org

(Difference between revisions)
(→Initial-Codon Switch)
(→Rare-Codon Switch)
Line 60: Line 60:
*[[Team:SJTU-BioX-Shanghai/Project/Subproject1/Modeling|See Project Modeling]]  
*[[Team:SJTU-BioX-Shanghai/Project/Subproject1/Modeling|See Project Modeling]]  
*[[Team:SJTU-BioX-Shanghai/Project/Subproject1/Results_2|See Project Result]]
*[[Team:SJTU-BioX-Shanghai/Project/Subproject1/Results_2|See Project Result]]
 +
-----
-----

Revision as of 14:12, 24 October 2011

    •


    Data Page

    Rare-Codon Switch

    Rare-Codon Switch: controlling rare tRNA amount

    • Data for our favorite new parts
    1. [http://partsregistry.org/wiki/index.php?title=Part:BBa_K567001 Main Page] - BBa_K567001 lacI -Ptrc-tRNAArg: The tRNAArg(drived from E.coli ArgW operon) is under the control of trc promoter, induced by IPTG.
    • Data for Pre-existing Parts
    1. [http://partsregistry.org/Part:BBa_I712019:Experience Experience] - BBa_I712019 Firefly luciferase (Anja Korenčič 2007)
    • We've Also Characterized the Following Part
    1. [http://partsregistry.org/wiki/index.php?title=Part:BBa_K5670002 Main Page] - BBa_K567002 sulA promoter-tRNAArg :The tRNAArg is under the control of sulApromoter induced by UV.


    Rare-Codon Switch: controlling aaRS

    • Data for our favorite new parts
    1. [http://partsregistry.org/wiki/index.php?title=Part:BBa_K567011 Main Page] - BBa_K567011 PT7-TDRS :Aspartyl aminoacyl tRNA synthetase without anticodon recognition domain under the control of T7 promoter and lac operator.
    2. [http://partsregistry.org/wiki/index.php?title=Part:BBa_K567012 Main Page] - BBa_K567012 tRNAAsp-AGG : The tRNAAsp-AGG with its anticodon mutated to CCU(base pairing rare codon AGG) and under the control of aspV promoter.
    • Data for Pre-existing Parts
    1. [http://partsregistry.org/Part:BBa_I13521:Experience Experience] - BBa_I13521 Ptet mRFP (Endy Lab 2005)
    • We've Also Characterized the Following Part
    1. [http://partsregistry.org/wiki/index.php?title=Part:BBa_K567017 Main Page] - BBa_K567017 PT7-RFP-6AGG


    Rare-Codon Switch: controlling rare codon number

    • Data for our favorite new parts
    1. [http://partsregistry.org/wiki/index.php?title=Part:BBa_K567011 Main Page] - BBa_K567011 PT7-TDRS :Aspartyl aminoacyl tRNA synthetase without anticodon recognition domain under the control of T7 promoter and lac operator.
    2. [http://partsregistry.org/wiki/index.php?title=Part:BBa_K567001 Main Page] - BBa_K567001 lacI -Ptrc-tRNAArg: The tRNAArg(drived from E.coli ArgW operon) is under the control of trc promoter, induced by IPTG.



    Stop-Codon Switch

    • Data for our favorite new parts
    1. [http://partsregistry.org/wiki/index.php?title=Part:BBa_K567011 Main Page] - BBa_K567011 PT7-TDRS :Aspartyl aminoacyl tRNA synthetase without anticodon recognition domain under the control of T7 promoter and lac operator.
    2. [http://partsregistry.org/wiki/index.php?title=Part:BBa_K567013 Main Page] - BBa_K567013 tRNAAsp-TAG : The tRNAAsp-TAG with its anticodon mutated to CUA (base pairing stop codon UAG) and under the control of aspV promoter.
    • We've Also Characterized the Following Part
    1. [http://partsregistry.org/wiki/index.php?title=Part:BBa_K567003 Main Page] - BBa_K567003 Pbla-Luc-TAG
    • We've Also Made the Following Part
    1. [http://partsregistry.org/wiki/index.php?title=Part:BBa_K567018 Main Page] - BBa_K567018 PT7-GFP-TAG-RFP


    Initial-Codon Switch

    • Data for our favorite new parts
    1. [http://partsregistry.org/wiki/index.php?title=Part:BBa_K567014 Main Page] - BBa_K567014 PT7-metGM : T7 promoter-metG(mutated). This biobrick is constructed by putting the mutated metG (Met-RS) under the control of T7 promoter and lac operator.
    2. [http://partsregistry.org/wiki/index.php?title=Part:BBa_K567015 Main Page] - BBa_K567015 PT7-metGN :T7 promoter-metG(truncated). This biobrick is constructed by putting the truncated metG (Met-RS) under the control of T7 promoter and lac operator.
    3. [http://partsregistry.org/wiki/index.php?title=Part:BBa_K567016 Main Page] - BBa_K567016 metY-CGA :This biobrick is constructed by mutating the anticodon of tRNAmet to TCG (base pairing codon CGA).
    • We've Also Characterized the Following Part
    1. [http://partsregistry.org/wiki/index.php?title=Part:BBa_K567020 Main Page] - BBa_K567020 KanR-CGA


    Retrieved from "http://2011.igem.org/Team:SJTU-BioX-Shanghai/Parts/Data_Page"