Team:SJTU-BioX-Shanghai/Parts/Data Page

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===Rare-Codon Switch: aaRS Modulator + Quantitative Reporter===
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===Rare-Codon Switch: controlling rare codon number===
This system is consisted of aaRS Modulator and Quantitative Reporter, which have been characterized separately above. The principle of this design is the same as the above two designs but showing a new combination.  
This system is consisted of aaRS Modulator and Quantitative Reporter, which have been characterized separately above. The principle of this design is the same as the above two designs but showing a new combination.  

Revision as of 14:01, 24 October 2011

    •


    Data Page

    Rare-Codon Switch

    Rare-Codon Switch: controlling rare tRNA amount

    • Data for our favorite new parts
    1. [http://partsregistry.org/wiki/index.php?title=Part:BBa_K567001 Main Page] - BBa_K567001 lacI -Ptrc-tRNAArg: The tRNAArg(drived from E.coli ArgW operon) is under the control of trc promoter, induced by IPTG.
    • Data for Pre-existing Parts
    1. [http://partsregistry.org/Part:BBa_I712019:Experience Experience] - BBa_I712019 Firefly luciferase (Anja Korenčič 2007)
    • We've Also Characterized the Following Part
    1. [http://partsregistry.org/wiki/index.php?title=Part:BBa_K5670002 Main Page] - BBa_K567002 sulA promoter-tRNAArg :The tRNAArg is under the control of sulApromoter induced by UV.


    Rare-Codon Switch: controlling aaRS

    • Data for our favorite new parts
    1. [http://partsregistry.org/wiki/index.php?title=Part:BBa_K567011 Main Page] - BBa_K567011 PT7-TDRS :Aspartyl aminoacyl tRNA synthetase without anticodon recognition domain under the control of T7 promoter and lac operator.
    2. [http://partsregistry.org/wiki/index.php?title=Part:BBa_K567012 Main Page] - BBa_K567012 tRNAAsp-AGG : The tRNAAsp-AGG with its anticodon mutated to CCU(base pairing rare codon AGG) and under the control of aspV promoter.
    • Data for Pre-existing Parts
    1. [http://partsregistry.org/Part:BBa_I13521:Experience Experience] - BBa_I13521 Ptet mRFP (Endy Lab 2005)
    • We've Also Characterized the Following Part
    1. [http://partsregistry.org/wiki/index.php?title=Part:BBa_K567017 Main Page] - BBa_K567017 PT7-RFP-6AGG


    Rare-Codon Switch: controlling rare codon number

    This system is consisted of aaRS Modulator and Quantitative Reporter, which have been characterized separately above. The principle of this design is the same as the above two designs but showing a new combination.

    11SJTU-data page-aaRS+luc.jpg


    Rare-Codon Switch: tRNA Modulator + Qualitative Reporter

    This system is consisted of tRNA Modulator and Qualitative Reporter, which have been characterized separately above. The principle of this design is the same as the above three designs but showing a new combination.

    11SJTU-data page-tRNA+RFP.jpg



    Stop-Codon Switch

    Stop-Codon Switch + Quantitative Reporter

    11SJTU-data page-stop+luc.jpg
    • Data for our favorite new parts
    1. [http://partsregistry.org/wiki/index.php?title=Part:BBa_K567011 Main Page] - BBa_K567011 PT7-TDRS :Aspartyl aminoacyl tRNA synthetase without anticodon recognition domain under the control of T7 promoter and lac operator.
    2. [http://partsregistry.org/wiki/index.php?title=Part:BBa_K567013 Main Page] - BBa_K567013 tRNAAsp-TAG : The tRNAAsp-TAG with its anticodon mutated to CUA (base pairing stop codon UAG) and under the control of aspV promoter.
    • We've Also Characterized the Following Part
    1. [http://partsregistry.org/wiki/index.php?title=Part:BBa_K567003 Main Page] - BBa_K567003 Pbla-Luc-TAG


    Stop-Codon Switch + Qualitative Reporter

    11SJTU-data page-stop+GFPRFP.jpg


    • We've Also Made the Following Part
    1. [http://partsregistry.org/wiki/index.php?title=Part:BBa_K567018 Main Page] - PT7-GFP-TAG-RFP


    Initial-Codon Switch

    11SJTU-data page-initial.jpg
    • Data for our favorite new parts
    1. [http://partsregistry.org/wiki/index.php?title=Part:BBa_K567014 Main Page] - BBa_K567014 PT7-metGM : T7 promoter-metG(mutated). This biobrick is constructed by putting the mutated metG (Met-RS) under the control of T7 promoter and lac operator.
    2. [http://partsregistry.org/wiki/index.php?title=Part:BBa_K567015 Main Page] - BBa_K567015 PT7-metGN :T7 promoter-metG(truncated). This biobrick is constructed by putting the truncated metG (Met-RS) under the control of T7 promoter and lac operator.
    3. [http://partsregistry.org/wiki/index.php?title=Part:BBa_K567016 Main Page] - BBa_K567016 metY-CGA :This biobrick is constructed by mutating the anticodon of tRNAmet to TCG (base pairing codon CGA).
    • We've Also Characterized the Following Part
    1. [http://partsregistry.org/wiki/index.php?title=Part:BBa_K567020 Main Page] - BBa_K567020 KanaR-CGA


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