Data Page

Rare-Codon Switch

Rare-Codon Switch: controlling rare tRNA amount

• Data for our favorite new parts

1. [http://partsregistry.org/wiki/index.php?title=Part:BBa_K567001 Main Page] - BBa_K567001 lacI -Ptrc-tRNAArg: The tRNAArg(drived from E.coli ArgW operon) is under the control of trc promoter, induced by IPTG.

• Data for Pre-existing Parts

1. [http://partsregistry.org/Part:BBa_I712019:Experience Experience] - BBa_I712019 Firefly luciferase (Anja Korenčič 2007)

• We've Also Characterized the Following Part

1. [http://partsregistry.org/wiki/index.php?title=Part:BBa_K5670002 Main Page] - BBa_K567002 sulA promoter-tRNAArg :The tRNAArg is under the control of sulApromoter induced by UV.

• See Project Design
• See Project Modeling
• See Project Result

Rare-Codon Switch: tRNA Modulator + Quantitative Reporter

• Data for our favorite new parts

1. [http://partsregistry.org/wiki/index.php?title=Part:BBa_K567001 Main Page] - BBa_K567001 lacI -Ptrc-tRNA^Arg :The tRNA^Arg(drived from E.coli ArgW operon) is under the control of trc promoter, induced by IPTG.

• Data for Pre-existing Parts

1. [http://partsregistry.org/Part:BBa_I712019:Experience Experience] - BBa_I712019 Firefly luciferase (Anja Korenčič 2007)

• We've Also Characterized the Following Part

1. PT7/Pbla-Luc-nAGG

If you want to know more about PT7/Pbla-Luc-nAGG and lacI-Ptrc-tRNA^Arg, click [http://partsregistry.org/wiki/index.php?title=Part:BBa_K567001 here]

If you want to know more about PT7/Pbla-Luc-nAGG and PT7-TDRS, click [http://partsregistry.org/wiki/index.php?title=Part:BBa_K567011 here]

2. [http://partsregistry.org/wiki/index.php?title=Part:BBa_K567002 Main Page] - BBa_K567002 sulA promoter-tRNA^Arg :The tRNA^Arg is under the control of sulA promoter induced by UV.

• See Project Design
• See Project Modeling
• See Project Result

Rare-Codon Switch: aaRS Modulator + Quantitative Reporter

This system is consisted of aaRS Modulator and Quantitative Reporter, which have been characterized separately above. The principle of this design is the same as the above two designs but showing a new combination.

• See Project Design
• See Project Result

Rare-Codon Switch: tRNA Modulator + Qualitative Reporter

This system is consisted of tRNA Modulator and Qualitative Reporter, which have been characterized separately above. The principle of this design is the same as the above three designs but showing a new combination.

• See Project Design
• See Project Result

Stop-Codon Switch

Stop-Codon Switch + Quantitative Reporter

• Data for our favorite new parts

1. [http://partsregistry.org/wiki/index.php?title=Part:BBa_K567011 Main Page] - BBa_K567011 PT7-TDRS :Aspartyl aminoacyl tRNA synthetase without anticodon recognition domain under the control of T7 promoter and lac operator.
2. [http://partsregistry.org/wiki/index.php?title=Part:BBa_K567013 Main Page] - BBa_K567013 tRNA^Asp-TAG : The tRNA^Asp-TAG with its anticodon mutated to CUA (base pairing stop codon UAG) and under the control of aspV promoter.

• We've Also Characterized the Following Part

1. [http://partsregistry.org/wiki/index.php?title=Part:BBa_K567003 Main Page] - BBa_K567003 Pbla-Luc-TAG

• See Project Design
• See Project Result

Stop-Codon Switch + Qualitative Reporter

• We've Also Made the Following Part

1. [http://partsregistry.org/wiki/index.php?title=Part:BBa_K567018 Main Page] - PT7-GFP-TAG-RFP

• See Project Design
• See Project Result

Initial-Codon Switch

• Data for our favorite new parts

1. [http://partsregistry.org/wiki/index.php?title=Part:BBa_K567014 Main Page] - BBa_K567014 PT7-metGM : T7 promoter-metG(mutated). This biobrick is constructed by putting the mutated metG (Met-RS) under the control of T7 promoter and lac operator.
2. [http://partsregistry.org/wiki/index.php?title=Part:BBa_K567015 Main Page] - BBa_K567015 PT7-metGN :T7 promoter-metG(truncated). This biobrick is constructed by putting the truncated metG (Met-RS) under the control of T7 promoter and lac operator.
3. [http://partsregistry.org/wiki/index.php?title=Part:BBa_K567016 Main Page] - BBa_K567016 metY-CGA :This biobrick is constructed by mutating the anticodon of tRNA^met to TCG (base pairing codon CGA).

• We've Also Characterized the Following Part

1. [http://partsregistry.org/wiki/index.php?title=Part:BBa_K567020 Main Page] - BBa_K567020 KanaR-CGA

• See Project Design
• See Project Result