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Team:British Columbia/Week9
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===beta-pinene & (-)-limonene synthase=== | ===beta-pinene & (-)-limonene synthase=== | ||
*Ran SDM-PCR | *Ran SDM-PCR | ||
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| + | ===3-Carene=== | ||
| + | Daisy did a single digest with EcoRI-HF to test if the EcoRI site is in the synthase or not. | ||
=Wednesday= | =Wednesday= | ||
Revision as of 18:34, 29 June 2011
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Week 9: Monday June 27 - Sunday July 3
Contents |
Monday
We created a set of lab rules due to common mistakes made in the lab over the past few weeks.
Vicki and Marianne will having training sessions throughout the week to show team members without much wetlab experience how to PCR, cast and run a gel, digest, ligate, transform, design primers, send miniprepped products to be sequenced.
Modeling: Jacob obtained the ARC program. Gurpal wants to create a wetlab flow chart to match the modeling.
Sent DspB (part from last year) to Grinnell College (a new iGEM team this year who requested our part) in the United States.
alpha-pinene synthase
- SDM-PCR worked! (Used the QuikChange SDM Kit protocol).
3-carene synthase
Daisy needs to make custom primers for sequencing purposes because the mutagen site is in the middle of the coding sequence and her flanking primers will only sequence 700-800bp into the sequence.
beta-pinene synthase
- Resuspended SDM forward and reverse primers
(-)-limonene synthase
- Resuspended SDM forward and reverse primers
Tuesday
Training: went over PCR protocol (SDM specific)
beta-pinene & (-)-limonene synthase
- Ran SDM-PCR
3-Carene
Daisy did a single digest with EcoRI-HF to test if the EcoRI site is in the synthase or not.
Wednesday
Training:
Thursday
Training:
