Team:SJTU-BioX-Shanghai/Project/Subproject1/Results

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Revision as of 16:37, 5 October 2011

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- Rare-Codon Switch
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Rare-Codon Switch

Results

Action: Combinations of Modulators and Reporters

Select a Modulator and a Reporter, then click to see results!

tRNA Modulator System

System Analysis

tRNA Modulator + Reporter for Qualitative Analysis

Action: when rare tRNA^Arg is over expressed, the amount of tRNA^Arg in the tRNA pool is largely increased, which facilitates ribosome to get through the tandem of AGG codons in the RFP's mRNA.

tRNA Modulator + Reporter for Quantitative Analysis

Action: when rare tRNA^Arg is over expressed, the amount of tRNA^Arg in the tRNA pool is largely increased, which facilitates ribosome to get through the tandem of AGG codons in the modified luciferase's mRNA.

aaRS Modulator System

aaRS Modulator + Reporter for Qualitative Analysis

Action: When TDRS is expressed under IPTG induction, it can charge tRNA^Asp-AGG with Asp. tRNA^Asp-AGG then recognizes the rare codon AGG on RFP's mRNA and add Asp to the polypeptide chain. RFP is expressed.

aaRS Modulator + Reporter for Quantitative analysis

Action: When TDRS is expressed under IPTG induction, it can charge tRNA^Asp-AGG with Asp. tRNA^Asp-AGG then recognizes the rare codon AGG on the modified luciferase's mRNA and add Asp to the polypeptide chain. Modified luciferase is expressed.

Revision as of 16:37, 5 October 2011 (view source) ChobitParrot (Talk \| contribs) (→tRNA Modulator System) ← Older edit		Revision as of 16:37, 5 October 2011 (view source) ChobitParrot (Talk \| contribs) (→tRNA Modulator System) Newer edit →
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	'''Action:''' when rare tRNA<sup>Arg</sup> is over expressed, the amount of tRNA<sup>Arg</sup> in the tRNA pool is largely increased, which facilitates ribosome to get through the tandem of AGG codons in the RFP's mRNA.		'''Action:''' when rare tRNA<sup>Arg</sup> is over expressed, the amount of tRNA<sup>Arg</sup> in the tRNA pool is largely increased, which facilitates ribosome to get through the tandem of AGG codons in the RFP's mRNA.
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	*[[Team:SJTU-BioX-Shanghai/Project/Subproject1/Results_2\|'''tRNA Modulator + Reporter for Quantitative Analysis ''']]		*[[Team:SJTU-BioX-Shanghai/Project/Subproject1/Results_2\|'''tRNA Modulator + Reporter for Quantitative Analysis ''']]