"
Team:Panama/29 June 2011
From 2011.igem.org
(Difference between revisions)
(Created page with "=='''June 29, 2011'''== We did a lot of work today like: 1. The miniprep of the strains containing the BioBrick rh1AB. 2. An electrophoresis in agarose gel at 1.5% in wich w...") |
(→June 29, 2011) |
||
| Line 9: | Line 9: | ||
3. Mean while some of us picked colonies with P+RBS and the GFP+Ter and transferred it to LB medium. | 3. Mean while some of us picked colonies with P+RBS and the GFP+Ter and transferred it to LB medium. | ||
| - | 4. Last attempt to inoculate the E. coli containing the rh1AB mutated from | + | 4. Last attempt to inoculate the E. coli containing the rh1AB mutated from 3/2/11 in LB medium but it didn´t grow again! =(. This time we putted the exact concentration of cloranfenicol (33 ug/ml). |
5. We did all the necessary calculations for the antibiotics concentrations. | 5. We did all the necessary calculations for the antibiotics concentrations. | ||
Latest revision as of 00:51, 29 September 2011
June 29, 2011
We did a lot of work today like:
1. The miniprep of the strains containing the BioBrick rh1AB.
2. An electrophoresis in agarose gel at 1.5% in wich we observed the plasmidic DNA.
3. Mean while some of us picked colonies with P+RBS and the GFP+Ter and transferred it to LB medium.
4. Last attempt to inoculate the E. coli containing the rh1AB mutated from 3/2/11 in LB medium but it didn´t grow again! =(. This time we putted the exact concentration of cloranfenicol (33 ug/ml).
5. We did all the necessary calculations for the antibiotics concentrations.
Rh1AB + psB1C3 Ectroforesis
