User contributions

From 2011.igem.org

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• 04:11, 29 September 2011 (diff | hist) Team:Panama/2 September 2011 ‎ (top)
• 04:10, 29 September 2011 (diff | hist) Team:Panama/2 September 2011 ‎
• 04:04, 29 September 2011 (diff | hist) Team:Panama/1 September 2011 ‎ (top)
• 04:00, 29 September 2011 (diff | hist) N Team:Panama/31 August 2011 ‎ (Created page with "The negative control is still growing. We plated bacteria without the resistance to see if the antibiotic is the one causing the problem.") (top)
• 03:58, 29 September 2011 (diff | hist) Team:Panama/14 August 2011 ‎ (top)
• 03:56, 29 September 2011 (diff | hist) N Team:Panama/14 August 2011 ‎ (Created page with "We did an electroforesis to confirm if we still have the rh1AB mutated BioBrick, but again, we discovered that the part has been degraded because so there is no more choice than ...")
• 03:46, 29 September 2011 (diff | hist) N Team:Panama/13 August 2011 ‎ (Created page with "Today we did the transformation of the P GEM-T+ rh1AB insert and resuspended the TU-DELFT BioBricks that we want to work with in our multi degrading hydrocarbons kit.") (top)
• 03:43, 29 September 2011 (diff | hist) Team:Panama/12 August 2011 ‎ (top)
• 03:39, 29 September 2011 (diff | hist) N Team:Panama/12 August 2011 ‎ (Created page with "We follow the [https://2011.igem.org/Pgem_Cloning'''cloning protocol'''] using pGEM-T. We incubate overnight at 4ºC in order to obtain more transformants.")
• 03:33, 29 September 2011 (diff | hist) N Team:Panama/11 August 2011 ‎ (Created page with "We purified the PCR´s Products of 1 and 3 with the [https://2011.igem.org/PCR_purification '''Roche´s High Pure PCR Product Purification kit.''']") (top)
• 03:33, 29 September 2011 (diff | hist) Team:Panama/11 September 2011 ‎ (Blanked the page) (top)
• 03:32, 29 September 2011 (diff | hist) N Team:Panama/9 August 2011 ‎ (Created page with "We observed the transformations, our results are the following: Petri Dishes with growht: LB+chloramphenicol+inducible Promoter, LB+Ampicilin+Inducible Promoter, LB+Ampicilin+C+...") (top)
• 03:32, 29 September 2011 (diff | hist) Team:Panama/9 September 2011 ‎ (Blanked the page)
• 03:31, 29 September 2011 (diff | hist) N Team:Panama/8 August 2011 ‎ (Created page with "We did an electrophoresis of the miniprep´s products (9/4/11) and PCR´s products (9/5/11). Trying to probe the primer´s couples, we run a P. aeruginosa genomic DNA´s PCR wit...") (top)
• 03:30, 29 September 2011 (diff | hist) Team:Panama/8 September 2011 ‎ (Blanked the page) (top)
• 03:30, 29 September 2011 (diff | hist) N Team:Panama/6 August 2011 ‎ (Created page with "We inoculate, bacterial colonies, on LB media: the sample 8 with an ampicilin's control") (top)
• 03:29, 29 September 2011 (diff | hist) Team:Panama/6 September 2011 ‎ (Blanked the page) (top)
• 03:19, 29 September 2011 (diff | hist) N Team:Panama/5 August 2011 ‎ (Created page with "We did a Pseudomonas aeruginosa´s PCR with genomic DNA, using a last year´s reaction (we had a very little amplification).") (top)
• 03:19, 29 September 2011 (diff | hist) Team:Panama/5 September 2011 ‎ (Blanked the page) (top)
• 03:17, 29 September 2011 (diff | hist) N Team:Panama/4 August 2011 ‎ (Created page with "A small group of Panama iGEM Team went to a presentation on Moscote´s High School We run a PCR using Roche´s master mix (template. P. aeruginosa genomic DNA) we hadn´t any re...") (top)
• 03:17, 29 September 2011 (diff | hist) Team:Panama/4 September 2011 ‎ (Blanked the page) (top)
• 03:16, 29 September 2011 (diff | hist) N Team:Panama/3 August 2011 ‎ (Created page with "We repeated the PCR of the Biobrick using other master mix, with the miniprep´s products obtained from the Biobrick ordered at MIT. We prepared a chloramphenicol stock (50mg/m...") (top)
• 03:16, 29 September 2011 (diff | hist) Team:Panama/3 September 2011 ‎ (Blanked the page) (top)
• 03:16, 29 September 2011 (diff | hist) N Team:Panama/2 August 2011 ‎ (Created page with "We did a digestion of the miniprep´s parts and a PCR of the Biobrick (We hadn´t any amplifications).") (top)
• 03:15, 29 September 2011 (diff | hist) Team:Panama/2 September 2011 ‎ (Blanked the page)
• 03:14, 29 September 2011 (diff | hist) N Team:Panama/1 August 2011 ‎ (Created page with "We did miniprep of cultures containing the biobrick that we ordered to iGEM Project (USA). At the same time, we prepared everything for an electrophoresis of these miniprep´s pr...") (top)
• 03:14, 29 September 2011 (diff | hist) Team:Panama/1 September 2011 ‎ (Blanked the page)
• 03:04, 29 September 2011 (diff | hist) Team:Panama/27 July 2011 ‎ (top)
• 03:04, 29 September 2011 (diff | hist) Team:Panama/27 July 2011 ‎
• 02:56, 29 September 2011 (diff | hist) N Team:Panama/25 July 2011 ‎ (Created page with "We have an important meeting with advisors and all the team. We discuss about the project, goals, assistance and some stuff. Also send an email asking for our Biobrick because we...") (top)
• 02:55, 29 September 2011 (diff | hist) Team:Panama/27 July 2011 ‎ (Blanked the page)
• 02:51, 29 September 2011 (diff | hist) N Team:Panama/27 July 2011 ‎ (Created page with "We have an important meeting with advisors and all the team. We discuss about the project, goals, assistance and some stuff. Also send an email asking for our Biobrick because we...")
• 02:50, 29 September 2011 (diff | hist) N Team:Panama/26 July 2011 ‎ (Created page with "We made an electrophoresis of every part that we have in the freezer because almost everything is almost not working for us. We figure out that our BioBricks were degraded.") (top)
• 02:47, 29 September 2011 (diff | hist) Team:Panama/22 July 2011 ‎ (top)
• 02:47, 29 September 2011 (diff | hist) Team:Panama/22 July 2011 ‎
• 02:46, 29 September 2011 (diff | hist) N File:Tabla9.png ‎ (top)
• 02:38, 29 September 2011 (diff | hist) N Team:Panama/22 July 2011 ‎ (Created page with "We take a sample of rh1AB purifying gen without mutation and doing a PCR because doesn’t have a lot and we have to digest with Pst1. On Monday we are going to do a mutagenesis....")
• 02:35, 29 September 2011 (diff | hist) Team:Panama/22 September 2011 ‎ (Blanked the page)
• 02:27, 29 September 2011 (diff | hist) Team:Panama/22 September 2011 ‎
• 02:25, 29 September 2011 (diff | hist) Team:Panama/22 September 2011 ‎
• 02:25, 29 September 2011 (diff | hist) N File:Tabla8.png ‎ (top)
• 02:18, 29 September 2011 (diff | hist) Team:Panama/22 September 2011 ‎
• 02:15, 29 September 2011 (diff | hist) N File:Tabla7.png ‎ (top)
• 01:47, 29 September 2011 (diff | hist) Team:Panama ‎
• 01:39, 29 September 2011 (diff | hist) N File:IGEM LOGO.png ‎ (top)
• 01:36, 29 September 2011 (diff | hist) Team:Panama ‎
• 01:23, 29 September 2011 (diff | hist) File:Comic strip.jpg ‎ (uploaded a new version of "File:Comic strip.jpg") (top)
• 01:16, 29 September 2011 (diff | hist) N Team:Panama/22 September 2011 ‎ (Created page with "We take a sample of rh1AB purifying gen without mutation and doing a PCR because doesn’t have a lot and we have to digest with Pst1. On Monday we are going to do a mutagenesis....")
• 01:11, 29 September 2011 (diff | hist) N File:Tabla6.png ‎ (top)
• 00:55, 29 September 2011 (diff | hist) PCR purification ‎

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