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From 2011.igem.org

We repeated the PCR of the Biobrick using other master mix, with the miniprep´s products obtained from the Biobrick ordered at MIT.

We prepared a chloramphenicol stock (50mg/mL), which was stored at -20 °C.

Primers used for PCR:

• RhT2b+RhT2a

• RhTbio2a+RhTbio2b

• RhTbioF1b+RhTbioF1a

• RhT1a+RhT1b

Program used for PCR:

We aliquoted Primers again (work solutions).

We did an electrophoresis of the PCR´s products on an agarose gel 1% TAE 1X (We hadn´t any results).

PCR (Probing primer´s effectiveness with a Pseudomonas aeruginosa´s genomic DNA)

Volume of master mix: 12.5 uL

Volume of DNA: 5 uL

Volume of Primers: 2.5 uL to everyone

Volume of H2O: 5.5 uL

Total Volume of reaction: 28 uL

We hadn´t any results, we realized that Taq Pol wasn´t capable of polymerize fragments most heavy than 1000 bp.