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From 2011.igem.org

June 29, 2011

We did a lot of work today like:

1. The miniprep of the strains containing the BioBrick rh1AB.

2. An electrophoresis in agarose gel at 1.5% in wich we observed the plasmidic DNA.

3. Mean while some of us picked colonies with P+RBS and the GFP+Ter and transferred it to LB medium.

4. Last attempt to inoculate the E. coli containing the rh1AB mutated from 3/2/11 in LB medium but it didn´t grow again! =(. This time we putted the exact concentration of cloranfenicol (33 ug/ml).

5. We did all the necessary calculations for the antibiotics concentrations.

Rh1AB + psB1C3 Ectroforesis