"
Team:British Columbia/Notebook/21 July 2011
From 2011.igem.org
(Difference between revisions)
(Created page with "{{Template:Notebook}} ==July 21 2011== <b>(-)-limonene</b> *DpnI digest yesterday's SDM PCR products at 37C for 4 hours *Gel verification of SDM PCR products show no bands. PCR...") |
(→July 21 2011) |
||
| Line 16: | Line 16: | ||
**Ligate backbone and part using Biobrick Ligation protocol | **Ligate backbone and part using Biobrick Ligation protocol | ||
**Transform assembly into DH5alpha cells | **Transform assembly into DH5alpha cells | ||
| + | |||
| + | <b>1,8-cineole</b> | ||
| + | *Assembling biobrick part. | ||
| + | **Second PCR did not work | ||
| + | ***Third PCR set to run overnight | ||
Latest revision as of 22:12, 1 August 2011
 |
 |
 |
 |
 |
July 21 2011
(-)-limonene
- DpnI digest yesterday's SDM PCR products at 37C for 4 hours
- Gel verification of SDM PCR products show no bands. PCR failed.
- PCR off the synthase to put onto yeast plasmid
- Troubleshooting: Use gradient, from 64C to 72C
- Gel verification of PCR products for all samples show 4 bands (same as yesterday). This may indicate, again, unspecific binding.
beta-pinene
- Assembling biobrick part
- Digest psb1C3 and synthase (part) with EcoRI and PstI using Biobrick Restriction Digest protocol
- Ligate backbone and part using Biobrick Ligation protocol
- Transform assembly into DH5alpha cells
1,8-cineole
- Assembling biobrick part.
- Second PCR did not work
- Third PCR set to run overnight
- Second PCR did not work
