Data Page

Rare-Codon Switch

Rare-Codon Switch: control with rare tRNA amount

• Data for our favorite new parts

1. [http://partsregistry.org/wiki/index.php?title=Part:BBa_K567001 Main Page] - BBa_K567001 lacI -Ptrc-tRNA^Arg: The tRNA^Arg(drived from E.coli ArgW operon) is under the control of trc promoter, induced by IPTG.

• Data for Pre-existing Parts

1. [http://partsregistry.org/Part:BBa_I712019:Experience Experience] - BBa_I712019 Firefly luciferase (Anja Korenčič 2007)

• We've Also Characterized the Following Part

1. [http://partsregistry.org/wiki/index.php?title=Part:BBa_K567002 Main Page] - BBa_K567002 sulA promoter-tRNA^Arg :The tRNA^Arg is under the control of sulA promoter induced by UV.

• See Project Design and Result

Rare-Codon Switch: control with aaRS

• Data for our favorite new parts

1. [http://partsregistry.org/wiki/index.php?title=Part:BBa_K567011 Main Page] - BBa_K567011 PT7-TDRS :Aspartyl aminoacyl tRNA synthetase without anticodon recognition domain under the control of T7 promoter and lac operator.
2. [http://partsregistry.org/wiki/index.php?title=Part:BBa_K567012 Main Page] - BBa_K567012 tRNA^Asp-AGG : The tRNA^Asp-AGG with its anticodon mutated to CCU(base pairing rare codon AGG) and under the control of aspV promoter.

• Data for Pre-existing Parts

1. [http://partsregistry.org/Part:BBa_I13521:Experience Experience] - BBa_I13521 Ptet mRFP (Endy Lab 2005)

• We've Also Characterized the Following Part

1. [http://partsregistry.org/wiki/index.php?title=Part:BBa_K567017 Main Page] - BBa_K567017 PT7-RFP-6AGG

• See Project Design and Result
• See Project Modeling

Rare-Codon Switch: control with rare codon number, locations and tandem copies

• Data for our favorite new parts

1. [http://partsregistry.org/wiki/index.php?title=Part:BBa_K567001 Main Page] - BBa_K567001 lacI -Ptrc-tRNA^Arg: The tRNA^Arg(drived from E.coli ArgW operon) is under the control of trc promoter, induced by IPTG.
2. [http://partsregistry.org/wiki/index.php?title=Part:BBa_K567004 Main Page] - PT7/Pbla-Luc-nAGG Date for control with rare codon number
3. [http://partsregistry.org/wiki/index.php?title=Part:BBa_K567021 Main Page] -PT7-Luc-2x4AGG(111bp) Date for control with rare codon number, locations and tandem copies

• See Project Design and Result
• See Project Modeling

Stop-Codon Switch

• Data for our favorite new parts

1. [http://partsregistry.org/wiki/index.php?title=Part:BBa_K567011 Main Page] - BBa_K567011 PT7-TDRS :Aspartyl aminoacyl tRNA synthetase without anticodon recognition domain under the control of T7 promoter and lac operator.
2. [http://partsregistry.org/wiki/index.php?title=Part:BBa_K567013 Main Page] - BBa_K567013 tRNA^Asp-TAG : The tRNA^Asp-TAG with its anticodon mutated to CUA (base pairing stop codon UAG) and under the control of aspV promoter.

• We've Also Characterized the Following Part

1. [http://partsregistry.org/wiki/index.php?title=Part:BBa_K567003 Main Page] - BBa_K567003 Pbla-Luc-TAG

• We've Also Made the Following Part

1. [http://partsregistry.org/wiki/index.php?title=Part:BBa_K567018 Main Page] - BBa_K567018 PT7-GFP-TAG-RFP

• See Project Design and Result

Initial-Codon Switch

• Data for our favorite new parts

1. [http://partsregistry.org/wiki/index.php?title=Part:BBa_K567014 Main Page] - BBa_K567014 PT7-metGM : T7 promoter-metG(mutated). This biobrick is constructed by putting the mutated metG (Met-RS) under the control of T7 promoter and lac operator.
2. [http://partsregistry.org/wiki/index.php?title=Part:BBa_K567015 Main Page] - BBa_K567015 PT7-metGN :T7 promoter-metG(truncated). This biobrick is constructed by putting the truncated metG (Met-RS) under the control of T7 promoter and lac operator.
3. [http://partsregistry.org/wiki/index.php?title=Part:BBa_K567016 Main Page] - BBa_K567016 metY-CGA :This biobrick is constructed by mutating the anticodon of tRNA^met to TCG (base pairing codon CGA).

• We've Also Characterized the Following Part

1. [http://partsregistry.org/wiki/index.php?title=Part:BBa_K567020 Main Page] - BBa_K567020 KanR-CGA

• See Project Design and Result

Positive FeedBack

• Data for our new parts

1. [http://partsregistry.org/wiki/index.php?title=Part:BBa_K567022 Main Page] - PT7-Luc-nAGG-tRNA(Arg). This biobrick is under the control of T7 promoter and lac operator.

• See this Application