Team:British Columbia/Notebook/Week 19

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==Mountain Pine Beetle and Saccharomyces cerevisiae Co-culturing==
==Mountain Pine Beetle and Saccharomyces cerevisiae Co-culturing==
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[[File:ubcigembeetleexp1.jpg | thumb | 300px | We ran the beetle transfer experiments not only with the mountain pine beetle, but also with the white pine weevil. Here we are getting the beetles from a generous benefactor. ]]
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[[File:ubcigembeetleexp1.jpg | 300px | We ran the beetle transfer experiments not only with the mountain pine beetle, but also with the white pine weevil. Here we are getting the beetles from a generous benefactor. ]]
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[[File:ubcigembeetleexp3.jpg | thumb | 300px | The White Pine Weevil]]
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[[File:ubcigembeetleexp3.jpg | 300px | The White Pine Weevil]]
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[[File:ubcigembeetleexp5.jpg | thumb | 300px |  
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[[File:ubcigembeetleexp5.jpg | 300px |  
Next came the fun part! We followed the beetle transfer protocol (see 'protocols'), transferring the beetle to the yeast plates, followed by placing them on a clean plate. After growing the plates (there was a lot of contamination), we analyzed the resultant yeast colonies to check for GFP and clonat resistance.]]
Next came the fun part! We followed the beetle transfer protocol (see 'protocols'), transferring the beetle to the yeast plates, followed by placing them on a clean plate. After growing the plates (there was a lot of contamination), we analyzed the resultant yeast colonies to check for GFP and clonat resistance.]]
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[[File:ubcigembeetleexp6.jpg | thumb | 300px | The deadly Mountain Pine Beetle!]]
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[[File:ubcigembeetleexp6.jpg | 300px | The deadly Mountain Pine Beetle!]]
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[[File:ubcigembeetleexp8.jpg | thumb | 300px | The MPB on a yeast plate.]]
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[[File:ubcigembeetleexp8.jpg | 300px | The MPB on a yeast plate.]]
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[[File:ubcigembeetleexp7.jpg | thumb | 300px |The beetles appeared to try to get the yeast off their backs by rolling around.]]  
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[[File:ubcigembeetleexp7.jpg | 300px |The beetles appeared to try to get the yeast off their backs by rolling around.]]  
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[[File:ubcigembeetleexp2.jpg | thumb | 200px | We noticed a brown spot on one of the beetles, and wanted to get a closer look. It turned out to be nothing, but then we heard we might see a mite! Alina searched long and hard for a mite, and found this beauty on one of the beetles.]]
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[[File:ubcigembeetleexp2.jpg | 200px | We noticed a brown spot on one of the beetles, and wanted to get a closer look. It turned out to be nothing, but then we heard we might see a mite! Alina searched long and hard for a mite, and found this beauty on one of the beetles.]]
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<html><iframe width="425" height="349" src="http://www.youtube.com/embed/-fXdy8plAR8?hl=en&fs=1" frameborder="0" allowfullscreen></iframe></html>

Revision as of 02:34, 29 October 2011

Team: British Columbia - 2011.igem.org