From 2011.igem.org
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| - | <html><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K517002"><b>Main Page</a> - Alpha-Pinene Synthase Gene, BBa_K517002:</b> The (-)-a-pinene synthase (PsTPS-Pin) was isolated from Sitka spruce and converts geranyl pyrophosphate to 83.4%(-)-alpha-pinene, 12.6%(-)-beta-pinene, 2.1% Linalool, 1.0% (-)-beta-phellandrene, 0.4% Camphene, and 0.4% myrcene. We characterized it by expression in C41 DE3 E. coli followed by a His-tag purification. The purified enzymes were assayed in vitro with GPP and sent for GC-MS, which confirmed alpha- and beta-pinene as products. </br></br> | + | <html> |
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| - | <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K517003"><b>Main Page</a> - Beta-Pinene Synthase Gene, BBa_K517003:</b> The (-)-b-pinene synthase (PsTPS-Pin2) synthase is a monoterpene synthase isolated from Sitka spruce converts geranyl pyrophosphate to 70.5%(-)-beta-pinene, 29.5%(-)-alpha-pinene. (-)-b-pinene synthase has been previously characterized by expression in C41 DE3 E. coli followed by a His-tag purification. The purified enzymes were assayed in vitro with GPP and sent for GC-MS, which confirmed alpha- and beta-pinene as products. </br></br>
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| - | <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K517000"><b>Main Page</a> - GAL galactose-inducible yeast promoter, BBa_K517000:</b> Promoter can be induced when exposed to galactose in the absence of glucose. We characterized this part by placing a reporter GFP gene downstream of the promoter and determined that GFP expression was up-regulated when the yeast was shifted to galactose media.</br></br>
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Revision as of 23:41, 27 October 2011
Team: British Columbia - 2011.igem.org
Project Accomplishments
Wet Laboratory Results
<h2>1. In vitro assay production of monoterpene in bacteria
GPP Assay
Modelling Results
Human Practice
We've also characterized the following parts
Main Page - GPD constitutive yeast promoter, BBa_K517001: Promoter for constitutively high expression. We characterized this part by placing a reporter GFP gene downstream of the promoter and determined that GFP was expressed regardless of whether the yeast was in glucose, raffinose or galactose media.
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