Team:Osaka/week12

From 2011.igem.org

(Difference between revisions)
Line 28: Line 28:
==October 20 (Thu)==
==October 20 (Thu)==
-
 
+
# Miniprep of yesterday's culture
 +
# Restriction digests of miniprepped parts
 +
# Electrophoresis of digests
 +
#* 118 x
 +
# Ligation
 +
#* 124(C):116(upstream)+117(downstream)+1-3A(vector)
 +
#* 125(C):116(upstream)+119(downstream)+1-3A(vector)
 +
#* 126(C):117(upstream)+119(downstream)+1-3A(vector)
 +
# To liquid culture:118
 +
# Transformation of ligation products
==October 21 (Fri)==
==October 21 (Fri)==
-
 
+
# Miniprep of yesterday's culture
 +
# Restriction digests of miniprepped parts
 +
# Electrophoresis of digests
 +
# Ligation
 +
#* 127(C):116(upstream)+118(downstream)+1-3A(vector)
 +
#* 128(C):117(upstream)+118(downstream)+1-3A(vector)
 +
#* 129(C):118(upstream)+119(downstream)+1-3A(vector)
 +
# To liquid culture:124,125,126
 +
# Transformation of ligation products
 +
# Plating (Tolerance assay)
==October 22 (Sat)==
==October 22 (Sat)==

Revision as of 13:22, 21 October 2011


October 16 (Sun)

Reginal Jamboree in HKUST

October 17 (Mon)

October 18 (Tue)

  1. Restriction digests
  2. Ligation
    • 116(T):105(upstream)+1-23L(downstream)+1-7A(vector)
    • 117(T):106(upstream)+1-23L(downstream)+1-7A(vector)
    • 118(T):107(upstream)+1-23L(downstream)+1-7A(vector)
    • 119(T):108(upstream)+1-23L(downstream)+1-7A(vector)
  3. Transformation of Ligation products

October 19 (Wed)

  1. To liquid culture:116,117,118,119
  2. Plating
  3. Ligation
    • 120(T):105(upstream)+1-23L(downstream)+1-5A(vector)
    • 121(T):106(upstream)+1-23L(downstream)+1-5A(vector)
    • 122(T):107(upstream)+1-23L(downstream)+1-5A(vector)
    • 123(T):108(upstream)+1-23L(downstream)+1-5A(vector)
  4. Transformation of ligation products

October 20 (Thu)

  1. Miniprep of yesterday's culture
  2. Restriction digests of miniprepped parts
  3. Electrophoresis of digests
    • 118 x
  4. Ligation
    • 124(C):116(upstream)+117(downstream)+1-3A(vector)
    • 125(C):116(upstream)+119(downstream)+1-3A(vector)
    • 126(C):117(upstream)+119(downstream)+1-3A(vector)
  5. To liquid culture:118
  6. Transformation of ligation products

October 21 (Fri)

  1. Miniprep of yesterday's culture
  2. Restriction digests of miniprepped parts
  3. Electrophoresis of digests
  4. Ligation
    • 127(C):116(upstream)+118(downstream)+1-3A(vector)
    • 128(C):117(upstream)+118(downstream)+1-3A(vector)
    • 129(C):118(upstream)+119(downstream)+1-3A(vector)
  5. To liquid culture:124,125,126
  6. Transformation of ligation products
  7. Plating (Tolerance assay)

October 22 (Sat)

Back to Notebook

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Retrieved from "http://2011.igem.org/Team:Osaka/week12"