Team:Osaka/week12

From 2011.igem.org

(Difference between revisions)
Line 18: Line 18:
==October 19 (Wed)==
==October 19 (Wed)==
-
 
+
# To liquid culture:116,117,118,119
 +
# Plating
 +
# Ligation
 +
#* 120(T):105(upstream)+1-23L(downstream)+1-5A(vector)
 +
#* 121(T):106(upstream)+1-23L(downstream)+1-5A(vector)
 +
#* 122(T):107(upstream)+1-23L(downstream)+1-5A(vector)
 +
#* 123(T):108(upstream)+1-23L(downstream)+1-5A(vector)
 +
# Transformation of ligation products
==October 20 (Thu)==
==October 20 (Thu)==

Revision as of 13:52, 19 October 2011


October 16 (Sun)

Reginal Jamboree in HKUST

October 17 (Mon)

October 18 (Tue)

  1. Restriction digests
  2. Ligation
    • 116(T):105(upstream)+1-23L(downstream)+1-7A(vector)
    • 117(T):106(upstream)+1-23L(downstream)+1-7A(vector)
    • 118(T):107(upstream)+1-23L(downstream)+1-7A(vector)
    • 119(T):108(upstream)+1-23L(downstream)+1-7A(vector)
  3. Transformation of Ligation products

October 19 (Wed)

  1. To liquid culture:116,117,118,119
  2. Plating
  3. Ligation
    • 120(T):105(upstream)+1-23L(downstream)+1-5A(vector)
    • 121(T):106(upstream)+1-23L(downstream)+1-5A(vector)
    • 122(T):107(upstream)+1-23L(downstream)+1-5A(vector)
    • 123(T):108(upstream)+1-23L(downstream)+1-5A(vector)
  4. Transformation of ligation products

October 20 (Thu)

October 21 (Fri)

October 22 (Sat)

Back to Notebook

week 1|2|3|4|5|6|7|8|9|10|11|12|13