Team:UNIST Korea/project/future work

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Revision as of 12:16, 4 October 2011

Team

Project

Experiment

Future work & Application

In the future our first effort will be to optimize the lysis module such that the Dpn enzymes are efficiently exploited by the synthetic microbes to eradicate its genetic material in a non-native environment.

On top of this, UNIST team will also introduce more controlled system into E.coli so that it can be more safe to the environment and easy to be handled in the laboratory.

We would like to introduce the following components into our Chop. coli in order to achieve our goal

Second light receptor

Quorum Sensing System

Two light signals before lysis would help in providing a more tight control and ensure that the device would still work even when one of them is mutated. Our team will also introduce a fourth sensing module to the Chop. coli using the quorum sensing system. In case of fermentor E. coli will experience its native original quorum sensing molecule, AI2 UNIST team is going to make that engineer E.coli to recognize different quorum sensing molecules such as AHL to differentiate between the fermentor and the real world. This system will also help achieve an effective and tight control of lysis device.

@@ Line 189: / Line 189: @@
 We would like to introduce the following components into our <i>Chop. coli</i> in order to achieve our goal<br/><br/>
-<center><b><font color=Red>Second light receptor</font></b></center><br/>
+<center><b><font color=Red>Second light receptor</font></b></center><br/><br/>
-<center><b><font color=Red>Quorum Sensing System</font></b></center><br/>
+<center><b><font color=Red>Quorum Sensing System</font></b></center><br/><br/>
-<center><b><font color=Red>fimE Processor</font></b></center><br/>
+Two light signals before lysis would help in providing a more tight control and ensure that the device would still work even when one of them is mutated.
 Our team  will also introduce a fourth sensing module to the <i>Chop. coli</i> using the <b><font size=4><font color=brown>quorum sensing system</font></b></font>. In case of fermentor <i>E. coli</i> will experience its native original quorum sensing molecule, AI2
@@ Line 197: / Line 199: @@
 This system will also help achieve an effective and tight control of lysis device. </br>
 <br/>
-<Font color=Red><b>Thirdly</font></b>, We have been trying to concentrate on more accurate control of gene expressions and effective decrease of leaky levels in our circuits by designing the <b><font size=4><font color=brown>temperature-dependent mRNA secondary structure</font></b></font>, but since it might not be working well completely for now probably this is expected to be accomplished with more clear experimental data, which will play a key role in the future construct. </br>
-<br/>
-<Font color=Red><b>Fourthly</b></font>, We also have been focusing on making good lysis module introduced in our <i>E.coli</i> using either <b><font size=4><font color=brown>Holin system</b></font></font> or <font size=4><font color=brown><b>Dpn system</b></font></font>. Unfortunately, the result was not that clear as much as we expected. Thus, for the greater progress, UNIST iGEM team is determined to work on the experiment much about lysis system in the future. </br>
-<br/>
-<Font color=Red><b>Lastly</b></font>, <font size=4><b><font color=brown>additional productive circuit</font></b></font> may join to the whole construct in the long future in order that <i>E.coli</i> can produce something genuinely valuable to the other things for applications because they may not be much worthy if they have nothing to produce anything although lots of controlled systems exist in the whole construct.
-For instance, as the additional productive systems for real applications, there might be systems containing the genes which are going to be much beneficial to the environment, or human health & well being, etc.
 </font>