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Team:UNIST Korea/project/future work
From 2011.igem.org
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In the future our first effort will be to optimize the lysis module such that the Dpn enzymes are efficiently exploited by the synthetic microbes to eradicate its genetic material in a non-native environment. <br/> <br/> | In the future our first effort will be to optimize the lysis module such that the Dpn enzymes are efficiently exploited by the synthetic microbes to eradicate its genetic material in a non-native environment. <br/> <br/> | ||
| - | + | On top of this, UNIST team will also introduce more controlled system into <i>E.coli</i> so that it can be more safe to the environment and easy to be handled in the laboratory. <br/> <br/> | |
| - | <b><font color=Red> | + | We would like to introduce the following components into our <i>Chop. coli</i> in order to achieve our goal<br/><br/> |
| + | <center><b><font color=Red>Second light receptor</font></b></center><br/> | ||
| + | <center><b><font color=Red>Quorum Sensing System</font></b></center><br/> | ||
| + | <center><b><font color=Red>fimE Processor</font></b></center><br/> | ||
| + | |||
| + | Our team will also introduce a fourth sensing module to the <i>Chop. coli</i> <b><font size=4><font color=brown>quorum sensing system</font></b></font> to engineered organism. However, the quorum sensing system which we are going to introduce to <i>E.coli</i> is somewhat different from that of original one that <i>E.coli</i> has already. In case of original quorum sensing of <i>E.coli</i> in the nature, they produce the molecules, AI2s which are referred to auto inducer molecules which will directly go through the membrane, make the complexes with other binding proteins in cytoplasm, and eventually will affect the certain gene on the chromosomal DNA so that it can induce the expression as for positive feedback loop. | ||
Unlike the original quorum sensing system, UNIST team is going to make that engineered <i>E.coli</i> can only recognize the signals from different quorum sensing molecules, AHLs which come especially from different species. | Unlike the original quorum sensing system, UNIST team is going to make that engineered <i>E.coli</i> can only recognize the signals from different quorum sensing molecules, AHLs which come especially from different species. | ||
Therefore, this system will also be the benefit to the effective control way of gene expressions. </br> | Therefore, this system will also be the benefit to the effective control way of gene expressions. </br> | ||
Revision as of 12:10, 4 October 2011
Future work & Application
In the future our first effort will be to optimize the lysis module such that the Dpn enzymes are efficiently exploited by the synthetic microbes to eradicate its genetic material in a non-native environment.
On top of this, UNIST team will also introduce more controlled system into E.coli so that it can be more safe to the environment and easy to be handled in the laboratory.
We would like to introduce the following components into our Chop. coli in order to achieve our goal
Our team will also introduce a fourth sensing module to the Chop. coli quorum sensing system to engineered organism. However, the quorum sensing system which we are going to introduce to E.coli is somewhat different from that of original one that E.coli has already. In case of original quorum sensing of E.coli in the nature, they produce the molecules, AI2s which are referred to auto inducer molecules which will directly go through the membrane, make the complexes with other binding proteins in cytoplasm, and eventually will affect the certain gene on the chromosomal DNA so that it can induce the expression as for positive feedback loop. Unlike the original quorum sensing system, UNIST team is going to make that engineered E.coli can only recognize the signals from different quorum sensing molecules, AHLs which come especially from different species. Therefore, this system will also be the benefit to the effective control way of gene expressions.
Thirdly, We have been trying to concentrate on more accurate control of gene expressions and effective decrease of leaky levels in our circuits by designing the temperature-dependent mRNA secondary structure, but since it might not be working well completely for now probably this is expected to be accomplished with more clear experimental data, which will play a key role in the future construct.
Fourthly, We also have been focusing on making good lysis module introduced in our E.coli using either Holin system or Dpn system. Unfortunately, the result was not that clear as much as we expected. Thus, for the greater progress, UNIST iGEM team is determined to work on the experiment much about lysis system in the future.
Lastly, additional productive circuit may join to the whole construct in the long future in order that E.coli can produce something genuinely valuable to the other things for applications because they may not be much worthy if they have nothing to produce anything although lots of controlled systems exist in the whole construct. For instance, as the additional productive systems for real applications, there might be systems containing the genes which are going to be much beneficial to the environment, or human health & well being, etc.
