Team:Osaka/week7

From 2011.igem.org

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#* 112(K):1-2M(upstream)+104(downstream)+1-5A(vector)
#* 112(K):1-2M(upstream)+104(downstream)+1-5A(vector)
# Transformation of ligation products
# Transformation of ligation products
 +
 +
[[Team:Osaka/Notebook|Back to Notebook]]
[[Team:Osaka/Notebook|Back to Notebook]]
week [[Team:Osaka/week1|1]]|[[Team:Osaka/week2|2]]|[[Team:Osaka/week3|3]]|[[Team:Osaka/week4|4]]|[[Team:Osaka/week5|5]]|[[Team:Osaka/week6|6]]|'''7'''|[[Team:Osaka/week8|8]]|[[Team:Osaka/week9|9]]|[[Team:Osaka/week10|10]]|[[Team:Osaka/week11|11]]|[[Team:Osaka/week12|12]]|[[Team:Osaka/week13|13]]|[[Team:Osaka/week14|14]]
week [[Team:Osaka/week1|1]]|[[Team:Osaka/week2|2]]|[[Team:Osaka/week3|3]]|[[Team:Osaka/week4|4]]|[[Team:Osaka/week5|5]]|[[Team:Osaka/week6|6]]|'''7'''|[[Team:Osaka/week8|8]]|[[Team:Osaka/week9|9]]|[[Team:Osaka/week10|10]]|[[Team:Osaka/week11|11]]|[[Team:Osaka/week12|12]]|[[Team:Osaka/week13|13]]|[[Team:Osaka/week14|14]]

Revision as of 05:48, 22 September 2011


Contents

September 11(Sun)

  1. Gel extraction of digests
  2. Miniprep of yesterday's culture
  3. Restriction digests of minipreppped parts
  4. Gel electrophoresis of digests
  5. Ligation
    • 103(C):PprM+1-3A(vector)
    • 104(C):RecA+1-3A(vector)
    • 105(A):12(upstream)+101(downstream)+1-1G(vector)
    • 106(A):12(upstream)+102(downstream)+1-1G(vector)
  6. Transformation of ligation products


September 12(Mon)

  1. To liquid culture:103,104,105,106

September 13(Tue)

  1. Miniprep of yesterday's culture
  2. Restriction digests of minipreppped parts
  3. Gel electrophoresis of digests
  4. Ligation
    • 107(A):12(upstream)+103(downstream)+1-1G(vector)
    • 108(A):12(upstream)+104(downstream)+1-1G(vector)
  5. Transformation of ligation products

September 14(Wed)

  1. To liquid culture:107,108

September 15(Thu)

  1. Miniprep of yesterday's culture
  2. Restriction digests of minipreppped parts
  3. Gel electrophoresis of digests
  4. To liquid culture:108

September 16(Fri)

  1. Miniprep of yesterday's culture
  2. Restriction digests of minipreppped parts
  3. Gel electrophoresis of digests


September 17(Sat)

  1. Ligation
    • 109(K):1-2M(upstream)+101(downstream)+1-5A(vector)
    • 110(K):1-2M(upstream)+102(downstream)+1-5A(vector)
    • 111(K):1-2M(upstream)+103(downstream)+1-5A(vector)
    • 112(K):1-2M(upstream)+104(downstream)+1-5A(vector)
  2. Transformation of ligation products


Back to Notebook

week 1|2|3|4|5|6|7|8|9|10|11|12|13|14

Retrieved from "http://2011.igem.org/Team:Osaka/week7"