Team:Osaka/week7

September 11 (Sun)

1. Gel extraction of digests
2. Miniprep of yesterday's culture
3. Restriction digests of minipreppped parts
4. Gel electrophoresis of digests
5. Ligation
   • 103(C):PprM+1-3A(vector)
   • 104(C):RecA+1-3A(vector)
   • 105(A):12(upstream)+101(downstream)+1-1G(vector)
   • 106(A):12(upstream)+102(downstream)+1-1G(vector)
6. Transformation of ligation products

September 12 (Mon)

1. To liquid culture:103,104,105,106

September 13 (Tue)

1. Miniprep of yesterday's culture
2. Restriction digests of minipreppped parts
3. Gel electrophoresis of digests
4. Ligation
   • 107(A):12(upstream)+103(downstream)+1-1G(vector)
   • 108(A):12(upstream)+104(downstream)+1-1G(vector)
5. Transformation of ligation products

September 14 (Wed)

1. To liquid culture:107,108

September 15 (Thu)

1. Miniprep of yesterday's culture
2. Restriction digests of minipreppped parts
3. Gel electrophoresis of digests
4. To liquid culture:108

September 16 (Fri)

1. Miniprep of yesterday's culture
2. Restriction digests of minipreppped parts
3. Gel electrophoresis of digests

September 17 (Sat)

1. Ligation
   • 109(K):1-2M(upstream)+101(downstream)+1-5A(vector)
   • 110(K):1-2M(upstream)+102(downstream)+1-5A(vector)
   • 111(K):1-2M(upstream)+103(downstream)+1-5A(vector)
   • 112(K):1-2M(upstream)+104(downstream)+1-5A(vector)
2. Transformation of ligation products

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