Template:Protocols

From 2011.igem.org

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<td><center><a href="/Team:British_Columbia/Protocols/Peace"><b>Peacekeeping</b><img src="https://static.igem.org/mediawiki/2011/3/34/Ubcwk8.jpg"></a></center><br></td>
 
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<td><center><a href="/Team:British_Columbia/Protocols/Sdm"><b>Site Directed Mutagenesis</b><img src="https://static.igem.org/mediawiki/2011/9/96/Ubcwk4.jpg"></a></center><br></td>
 
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<td><center><a href="/Team:British_Columbia/Protocols/Bacteria"><b>Bacterial Transformation</b><img src="https://static.igem.org/mediawiki/2011/1/19/Ubcsmilie.jpg"></a></center><br></td>
 
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<td><center><a href="/Team:British_Columbia/Protocols/Yeast"><b>Yeast Transformation</b><img src="https://static.igem.org/mediawiki/2011/0/0d/Ubcigemyeastransform.jpg"></a></center><br></td>
 
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<td><center><a href="/Team:British_Columbia/Protocols/Gcms"><b>GC-MS</b><img src="https://static.igem.org/mediawiki/2011/c/c2/Ubcigemgcms.jpg"></a></center><br></td>
 
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<a href="/Team:British_Columbia/Protocols/Peace"><b>iGEM Tips for Success</a><br></b>
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Things to think about when designing your project and experiments, as well as general safety rules.<br><br>
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<b><a href="/Team:British_Columbia/Protocols/Sdm">Site Directed Mutagenesis</a><br></b>
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A molecular biology technique in which a mutation is created at a defined site in a DNA molecule.<br><br>
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<b><a href="/Team:British_Columbia/Protocols/Bacteria">Bacterial Standard Operating Protocols</a><br></b>
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How to prepare competent cells, transform your construct into competent cells, and express your protein from a lac promoter.<br><br>
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<b><a href="/Team:British_Columbia/Protocols/Yeast">Yeast Standard Operating Protocols</a><br></b>
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How to transform your construct into yeast, obtain crude extract for SDS-PAGE, and perform GFP fixation for microscopy and fluorescence-activated cell sorting.<br><br>
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<b><a href="/Team:British_Columbia/Protocols/Gcms">Gas Chromatography-Mass Spectrometry (GC-MS)</a><br></b>
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A method that combines the features of gas-liquid chromatography and mass spectrometry to identify different substances within a test sample.<br><br>
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<b><a href="/Team:British_Columbia/Protocols/Beetle">Beetle Transfer Experiments</a><br></b>
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Preliminary experiments to probe the efficiency of transferring yeast via beetle vector.<br><br>
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<b><a href="/Team:British_Columbia/Protocols/Fungi">Yeast-Fungi Co-culture Experiments</a><br></b>
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Preliminary experiments to investigate the competitive interactions between yeast and the Bluestain Fungus.
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Latest revision as of 17:46, 18 October 2011

Team: British Columbia - 2011.igem.org

iGEM Tips for Success
Things to think about when designing your project and experiments, as well as general safety rules.

Site Directed Mutagenesis
A molecular biology technique in which a mutation is created at a defined site in a DNA molecule.

Bacterial Standard Operating Protocols
How to prepare competent cells, transform your construct into competent cells, and express your protein from a lac promoter.

Yeast Standard Operating Protocols
How to transform your construct into yeast, obtain crude extract for SDS-PAGE, and perform GFP fixation for microscopy and fluorescence-activated cell sorting.

Gas Chromatography-Mass Spectrometry (GC-MS)
A method that combines the features of gas-liquid chromatography and mass spectrometry to identify different substances within a test sample.

Beetle Transfer Experiments
Preliminary experiments to probe the efficiency of transferring yeast via beetle vector.

Yeast-Fungi Co-culture Experiments
Preliminary experiments to investigate the competitive interactions between yeast and the Bluestain Fungus.