Team:SJTU-BioX-Shanghai

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<div id="cnt"><h2>Countdown</h2><embed src="http://www.oneplusyou.com/bb/files/countdown/countdown.swf?co=0066ff&bgcolor=eeeeee&date_month=10&date_day=15&date_year=2011&un=iGEM World Jamboree 2011&size=normal&mo=11&da=5&yr=2011" type="application/x-shockwave-flash" pluginspage="http://www.macromedia.com/go/getflashplayer" width="200 px" height="60 px" wmode="transparent"></embed></div>
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<div id="vst"><h2>Visitors</h2><a href="http://www2.clustrmaps.com/user/8c3de420"><img src="http://www2.clustrmaps.com/stats/maps-no_clusters/2011.igem.org-Team-SJTU-BioX-Shanghai-thumb.jpg" alt="Locations of visitors to this page" /></a></div>
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<div id="sps"><h2>Sponsors</h2>
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<a href="http://www.merckmillipore.com/millipore"><img src="/wiki/images/e/ec/11SJTU_merck.jpg" alt="Merck millipore" height="80 px" /></a><h5>Merck Millipore</h5></div>
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<a href="http://www.sjtu.edu.cn"><img src="/wiki/images/7/70/11SJTU-SJTU-logo.jpg" alt="Shanghai Jiao Tong University" height="50 px" margin="auto" /></a><h5>Shanghai Jiao Tong University</h5></div>
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<a href="http://www.bio-x.cn"><img src="/wiki/images/8/81/11SJTU_bio-x.jpg" alt="Bio-X Institutes" height="50px"/></a><h5>Bio-X Institutes</h5></div>
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<a href="http://www.genscript.com/"><img src="/wiki/images/7/7c/11SJTU-Genscript.jpg" alt="GenScript" height="50px"/></a><h5>GenScript</h5></div>
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<div class="title"><b>The Project: Codon Switch Controlling Protein Biosynthesis</b></div>
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<p>SJTU-BioX-Shanghai iGEM team is designing a set of Codon-Switches that regulate target protein biosynthesis (translation).</p>
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<p>In our <a href="/Team:SJTU-BioX-Shanghai/Project/Subproject1"><b>Rare-Codon Switch</b></a>, the translation of the protein can be finely turned up/down with the control of  <a href="/Team:SJTU-BioX-Shanghai/Project/Subproject1-1">rare tRNA amount</a>,  <a href="/Team:SJTU-BioX-Shanghai/Project/Subproject1-2">aaRS that charges the rare tRNA</a> and <a href="/Team:SJTU-BioX-Shanghai/Project/Subproject1-3">rare codons</a>.
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<p>Besides, our device can be made into switches that can be turned on/off without background noise in two ways. One is to use stop codon as the controlling element, the <a href="/Team:SJTU-BioX-Shanghai/Project/Subproject2"><b>Stop-Codon Switch</b></a>. The other is to use any codon but the original start codon to initiate translation, the <a href="/Team:SJTU-BioX-Shanghai/Project/Subproject3"><b>Initial-Codon Switch</b></a>.</p>
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<img src="/wiki/images/e/e2/11SJTU_home_fig1.jpg"  alt="Project" style="float:right;"/>
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<p>Our design has expanded the regulating tools for synthetic biology and introduced brand-new methods for protein function analysis. See more about project applications, click <a href="/Team:SJTU-BioX-Shanghai/Project/Application"><b>here</b></a>.</p>
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    <div><li>Project Description</li>
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    <h2>A Synthetic-Biological System Controlling Translation Process</h2>
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    <p>2011 SJTU-BioX iGEM team is designing a system in which we will be able to control the expression of the target gene on the level of translation. Our system has provided a brand-new approach that can be universally applied to any protein expression system. In our system we can strictly switch on/off the translation of the target gene; we can selectively translate a part of the whole open reading frame; we can also shift certain amino acid residues to any of the 20 possible alternatives without altering the sequence of the gene. Our significant design offers a multi-purpose and universal way to regulate gene expression on translation level, to selectively express part of a gene, to introduce mutation to the target position of a protein and to study the important residues or domains of the target protein.</p>  
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<div class="cell"><li>TEAM</li></div>
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<div class="cell"><li>PARTS</li></div>
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<div class="cell"><li>MODELING</li></div>
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<div class="cell"><li>NOTEBOOK</li></div>
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<div class="cell"><li>SAFETY</li></div>
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<div class="cell"><li>HUMAN PRACTICE</li></div>
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<div class="cell"><li>ACKNOWLEDGEMENTS</li></div>
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<div class="cell"><li>SPONSORS</li></div>
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<div class="title"><b>Achievements</b></div>
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<img src="/wiki/images/3/3a/11SJTU_cheering.jpg" style="float:right;margin:10px;">
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<b>Gold Medal</b> and <b>Best New BioBrick Part or Device, Engineered</b>: <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K567011">BBa_K567011</a> & <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K567012">BBa_K567012</a> at Asia Jamboree. Advancing to World Champion!</p>
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<p><img src="/wiki/images/7/7f/11SJTU_r.png"  alt="right" />
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Constructed three Codon Switches that controls protein biosynthesis, <a href="/Team:SJTU-BioX-Shanghai/Project/Subproject1">Rare-Codon Switch</a>, <a href="/Team:SJTU-BioX-Shanghai/Project/Subproject2">Stop-Codon Switch</a> and <a href="/Team:SJTU-BioX-Shanghai/Project/Subproject3">Initial-Codon Switch</a>. </p>
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<p><img src="/wiki/images/7/7f/11SJTU_r.png"  alt="right" />
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Used <a href="/Team:SJTU-BioX-Shanghai/Project/Subproject1">Rare-Codon Switch</a> to turn up/down protein biosynthesis by controlling <a href="/Team:SJTU-BioX-Shanghai/Project/Subproject1-1">rare tRNA amount</a>, <a href="/Team:SJTU-BioX-Shanghai/Project/Subproject1-2">aaRS</a> and <a href="/Team:SJTU-BioX-Shanghai/Project/Subproject1-3">the number of rare codons.</a> </p>
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<p><img src="/wiki/images/7/7f/11SJTU_r.png"  alt="right" />
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Built our Rare-Codon Switch <a href="/Team:SJTU-BioX-Shanghai/Project/Subproject1/Modeling"><i>in silic</i></a> and demonstrated the reliability of the model through our experiment results.
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</p>
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<p><img src="/wiki/images/7/7f/11SJTU_r.png"  alt="right" />
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Constructed two strict switches, <a href="/Team:SJTU-BioX-Shanghai/Project/Subproject2">Stop-Codon Switch</a> and <a href="/Team:SJTU-BioX-Shanghai/Project/Subproject3">Initial-Codon Switch</a>, that can turn on/off protein biosynthesis. </p>
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<p><img src="/wiki/images/7/7f/11SJTU_r.png"  alt="right" />
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Demonstrated <b>device application</b> by constructing and successfully testing <a href="/Team:SJTU-BioX-Shanghai/Project/Application">positive feedback</a> with our device in metabolic pathway. </p>
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<p><img src="/wiki/images/7/7f/11SJTU_r.png"  alt="right" />
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Constructed <a href="/Team:SJTU-BioX-Shanghai/Parts">27 biobricks</a> in our 3 sub-projects.
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</p>
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<p><img src="/wiki/images/7/7f/11SJTU_r.png"  alt="right" />
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Analysed the structures of 18 aaRS with <a href="/Team:SJTU-BioX-Shanghai/Project/Application-3">structural biological methods</a>. Designed structures of 14 modified aaRS deprived of their anticodon recognition ability.
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</p>
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<p><img src="/wiki/images/7/7f/11SJTU_r.png"  alt="right" />
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Expanded the <a href="/Team:SJTU-BioX-Shanghai/Project/Application">regulating tools</a> for synthetic biology and developed two brand-new ways that facilitate the study of protein function.
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</p>
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<p><img src="/wiki/images/7/7f/11SJTU_r.png"  alt="right" />
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Designed our Human Practice Project: <a href="/Team:SJTU-BioX-Shanghai/Human_practice">T-PostCards</a> to promote our views on synthetic biology through communications and connections.
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</p>
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<p><img src="/wiki/images/7/7f/11SJTU_r.png"  alt="right" />
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Promoted synthetic biology <a href="/Team:SJTU-BioX-Shanghai/Human_practice_2">in high school class</a>. Designed a questionnaire and did a survey on high school students' understandings and attitudes towards synthetic biology.
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</p>
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<p><img src="/wiki/images/7/7f/11SJTU_r.png"  alt="right" />
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Attended the <b>2011 China Meet Up</b> and presented our project idea to other iGEMer and advisors. </p>
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Latest revision as of 03:52, 29 October 2011

    •

    Countdown

    Visitors

    Locations of visitors to this page

    Sponsors

    Merck millipore
    Merck Millipore
    Shanghai Jiao Tong University
    Shanghai Jiao Tong University
    Bio-X Institutes
    Bio-X Institutes
    GenScript
    GenScript
    The Project: Codon Switch Controlling Protein Biosynthesis

    SJTU-BioX-Shanghai iGEM team is designing a set of Codon-Switches that regulate target protein biosynthesis (translation).

    In our Rare-Codon Switch, the translation of the protein can be finely turned up/down with the control of rare tRNA amount, aaRS that charges the rare tRNA and rare codons.

    Besides, our device can be made into switches that can be turned on/off without background noise in two ways. One is to use stop codon as the controlling element, the Stop-Codon Switch. The other is to use any codon but the original start codon to initiate translation, the Initial-Codon Switch.

    Project

    Our design has expanded the regulating tools for synthetic biology and introduced brand-new methods for protein function analysis. See more about project applications, click here.

    Team
    Parts
    Human practice
    Achievements

    right Gold Medal and Best New BioBrick Part or Device, Engineered: BBa_K567011 & BBa_K567012 at Asia Jamboree. Advancing to World Champion!

    right Constructed three Codon Switches that controls protein biosynthesis, Rare-Codon Switch, Stop-Codon Switch and Initial-Codon Switch.

    right Used Rare-Codon Switch to turn up/down protein biosynthesis by controlling rare tRNA amount, aaRS and the number of rare codons.

    right Built our Rare-Codon Switch in silic and demonstrated the reliability of the model through our experiment results.

    right Constructed two strict switches, Stop-Codon Switch and Initial-Codon Switch, that can turn on/off protein biosynthesis.

    right Demonstrated device application by constructing and successfully testing positive feedback with our device in metabolic pathway.

    right Constructed 27 biobricks in our 3 sub-projects.

    right Analysed the structures of 18 aaRS with structural biological methods. Designed structures of 14 modified aaRS deprived of their anticodon recognition ability.

    right Expanded the regulating tools for synthetic biology and developed two brand-new ways that facilitate the study of protein function.

    right Designed our Human Practice Project: T-PostCards to promote our views on synthetic biology through communications and connections.

    right Promoted synthetic biology in high school class. Designed a questionnaire and did a survey on high school students' understandings and attitudes towards synthetic biology.

    right Attended the 2011 China Meet Up and presented our project idea to other iGEMer and advisors.

    Retrieved from "http://2011.igem.org/Team:SJTU-BioX-Shanghai"