"
Team:British Columbia/Notebook/29 June 2011
From 2011.igem.org
(Difference between revisions)
(→beta-pinene & (-)-limonene synthase) |
(→1,8-Cineole) |
||
| Line 9: | Line 9: | ||
====1,8-Cineole==== | ====1,8-Cineole==== | ||
Jacob transformed competent cells with his SDM-PCR product. | Jacob transformed competent cells with his SDM-PCR product. | ||
| - | + | Also ran SDM-PCR on variant of original synthase. | |
Latest revision as of 20:19, 4 July 2011
 |
 |
 |
 |
 |
June 29 2011
Training: The plan was to make kanamycin plates, but this was at a stand-still as we had troubles locating the tube of kanamycin sulfate salt.
beta-pinene & (-)-limonene synthase
- Verified yesterday's SDM-PCR products through gel electrophoresis. No bands were present in any of the lanes.
- Troubleshooting: The dNTP could have been too old/contaminated (we had troubles with this particular tube of dNTP from last year); the concentrations for the reagents used were not optimized - Marianne researched protocols and optimized the SDM-PCR protocol. We (Vicki and Marianne) will test it tomorrow.
1,8-Cineole
Jacob transformed competent cells with his SDM-PCR product. Also ran SDM-PCR on variant of original synthase.
