"
Team:British Columbia/Notebook/4 July 2011
From 2011.igem.org
(Difference between revisions)
(Created page with "==July 04 2011== ====IDI1, HMG2, erg20-2==== *Validated competent cells by transforming with pet100 vector containing Cineole synthase. They are in working order. *Amplified 5 ...") |
(→July 04 2011) |
||
| (6 intermediate revisions not shown) | |||
| Line 1: | Line 1: | ||
| + | {{Template:Notebook}} | ||
==July 04 2011== | ==July 04 2011== | ||
| - | + | <b>IDI1, HMG2, erg20-2</b> | |
| - | + | ||
*Validated competent cells by transforming with pet100 vector containing Cineole synthase. They are in working order. | *Validated competent cells by transforming with pet100 vector containing Cineole synthase. They are in working order. | ||
*Amplified 5 plasmids received from Greece (IDI1, HMG2, p300, Cin, Sab). NOTE: did not properly centrifuge during DNA precipitation stage. Will validate plasmids in yeast transformations to make sure plasmids are in working order. | *Amplified 5 plasmids received from Greece (IDI1, HMG2, p300, Cin, Sab). NOTE: did not properly centrifuge during DNA precipitation stage. Will validate plasmids in yeast transformations to make sure plasmids are in working order. | ||
*Awaiting materials from France (erg20-2). | *Awaiting materials from France (erg20-2). | ||
| + | <p><b>1,8-Cineole</b></p> | ||
| + | *Ran a gel to test restriction digest on variant SDM-PCR product. Looks like the SDM was successful! | ||
| + | <p><b>beta-pinene & (-)-limonene</b></p> | ||
| + | *SDM beta-pinene again by using both Pfu and Taq. | ||
| + | *Gel verification shows a smear. | ||
| + | *Troubleshooting: run a gel on the miniprep of the beta-pinene plasmid received from Rafael to make sure that the DNA hasn't been contaminated/digested. | ||
Latest revision as of 20:04, 30 July 2011
 |
 |
 |
 |
 |
July 04 2011
IDI1, HMG2, erg20-2
- Validated competent cells by transforming with pet100 vector containing Cineole synthase. They are in working order.
- Amplified 5 plasmids received from Greece (IDI1, HMG2, p300, Cin, Sab). NOTE: did not properly centrifuge during DNA precipitation stage. Will validate plasmids in yeast transformations to make sure plasmids are in working order.
- Awaiting materials from France (erg20-2).
1,8-Cineole
- Ran a gel to test restriction digest on variant SDM-PCR product. Looks like the SDM was successful!
beta-pinene & (-)-limonene
- SDM beta-pinene again by using both Pfu and Taq.
- Gel verification shows a smear.
- Troubleshooting: run a gel on the miniprep of the beta-pinene plasmid received from Rafael to make sure that the DNA hasn't been contaminated/digested.
