From 2011.igem.org
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| ==Positive FeedBack== | | ==Positive FeedBack== |
| + | *'''Data for our new parts''' |
| + | # [http://partsregistry.org/wiki/index.php?title=Part:BBa_K567022 Main Page] - PT7-Luc-nAGG-tRNA(Arg). This biobrick is under the control of T7 promoter and'' lac'' operator. |
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| + | *[[Team:SJTU-BioX-Shanghai/Project/Application|See this Application]] |
Latest revision as of 03:57, 29 October 2011
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Data Page
Rare-Codon Switch
Rare-Codon Switch: control with rare tRNA amount
- Data for our favorite new parts
- [http://partsregistry.org/wiki/index.php?title=Part:BBa_K567001 Main Page] - BBa_K567001 lacI -Ptrc-tRNAArg: The tRNAArg(drived from E.coli ArgW operon) is under the control of trc promoter, induced by IPTG.
- Data for Pre-existing Parts
- [http://partsregistry.org/Part:BBa_I712019:Experience Experience] - BBa_I712019 Firefly luciferase (Anja Korenčič 2007)
- We've Also Characterized the Following Part
- [http://partsregistry.org/wiki/index.php?title=Part:BBa_K567002 Main Page] - BBa_K567002 sulA promoter-tRNAArg :The tRNAArg is under the control of sulA promoter induced by UV.
Rare-Codon Switch: control with aaRS
- Data for our favorite new parts
- [http://partsregistry.org/wiki/index.php?title=Part:BBa_K567011 Main Page] - BBa_K567011 PT7-TDRS :Aspartyl aminoacyl tRNA synthetase without anticodon recognition domain under the control of T7 promoter and lac operator.
- [http://partsregistry.org/wiki/index.php?title=Part:BBa_K567012 Main Page] - BBa_K567012 tRNAAsp-AGG : The tRNAAsp-AGG with its anticodon mutated to CCU(base pairing rare codon AGG) and under the control of aspV promoter.
- Data for Pre-existing Parts
- [http://partsregistry.org/Part:BBa_I13521:Experience Experience] - BBa_I13521 Ptet mRFP (Endy Lab 2005)
- We've Also Characterized the Following Part
- [http://partsregistry.org/wiki/index.php?title=Part:BBa_K567017 Main Page] - BBa_K567017 PT7-RFP-6AGG
Rare-Codon Switch: control with rare codon number, locations and tandem copies
- Data for our favorite new parts
- [http://partsregistry.org/wiki/index.php?title=Part:BBa_K567001 Main Page] - BBa_K567001 lacI -Ptrc-tRNAArg: The tRNAArg(drived from E.coli ArgW operon) is under the control of trc promoter, induced by IPTG.
- [http://partsregistry.org/wiki/index.php?title=Part:BBa_K567004 Main Page] - PT7/Pbla-Luc-nAGG Date for control with rare codon number
- [http://partsregistry.org/wiki/index.php?title=Part:BBa_K567021 Main Page] -PT7-Luc-2x4AGG(111bp) Date for control with rare codon number, locations and tandem copies
Stop-Codon Switch
- Data for our favorite new parts
- [http://partsregistry.org/wiki/index.php?title=Part:BBa_K567011 Main Page] - BBa_K567011 PT7-TDRS :Aspartyl aminoacyl tRNA synthetase without anticodon recognition domain under the control of T7 promoter and lac operator.
- [http://partsregistry.org/wiki/index.php?title=Part:BBa_K567013 Main Page] - BBa_K567013 tRNAAsp-TAG : The tRNAAsp-TAG with its anticodon mutated to CUA (base pairing stop codon UAG) and under the control of aspV promoter.
- We've Also Characterized the Following Part
- [http://partsregistry.org/wiki/index.php?title=Part:BBa_K567003 Main Page] - BBa_K567003 Pbla-Luc-TAG
- We've Also Made the Following Part
- [http://partsregistry.org/wiki/index.php?title=Part:BBa_K567018 Main Page] - BBa_K567018 PT7-GFP-TAG-RFP
Initial-Codon Switch
- Data for our favorite new parts
- [http://partsregistry.org/wiki/index.php?title=Part:BBa_K567014 Main Page] - BBa_K567014 PT7-metGM : T7 promoter-metG(mutated). This biobrick is constructed by putting the mutated metG (Met-RS) under the control of T7 promoter and lac operator.
- [http://partsregistry.org/wiki/index.php?title=Part:BBa_K567015 Main Page] - BBa_K567015 PT7-metGN :T7 promoter-metG(truncated). This biobrick is constructed by putting the truncated metG (Met-RS) under the control of T7 promoter and lac operator.
- [http://partsregistry.org/wiki/index.php?title=Part:BBa_K567016 Main Page] - BBa_K567016 metY-CGA :This biobrick is constructed by mutating the anticodon of tRNAmet to TCG (base pairing codon CGA).
- We've Also Characterized the Following Part
- [http://partsregistry.org/wiki/index.php?title=Part:BBa_K567020 Main Page] - BBa_K567020 KanR-CGA
Positive FeedBack
- [http://partsregistry.org/wiki/index.php?title=Part:BBa_K567022 Main Page] - PT7-Luc-nAGG-tRNA(Arg). This biobrick is under the control of T7 promoter and lac operator.
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