Team:SJTU-BioX-Shanghai

From 2011.igem.org

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<div class="title"><b>The Project: Codon Switch Controlling Protein Biosynthesis</b></div>
<div class="title"><b>The Project: Codon Switch Controlling Protein Biosynthesis</b></div>
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<div class="title"><b>Achievements</b></div>
<div class="title"><b>Achievements</b></div>
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<img src="/wiki/images/3/3a/11SJTU_cheering.jpg" style="float:right;margin:10px;">
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<p><img src="/wiki/images/7/7f/11SJTU_r.png"  alt="right" />
<p><img src="/wiki/images/7/7f/11SJTU_r.png"  alt="right" />
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<b>Gold Medal</b> and <b>Best New BioBrick Part or Device, Engineered</b>: BBa_K567011 & BBa_K567012 at Asia Jamboree. Advancing to World Champion!</p>
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<b>Gold Medal</b> and <b>Best New BioBrick Part or Device, Engineered</b>: <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K567011">BBa_K567011</a> & <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K567012">BBa_K567012</a> at Asia Jamboree. Advancing to World Champion!</p>
<p><img src="/wiki/images/7/7f/11SJTU_r.png"  alt="right" />
<p><img src="/wiki/images/7/7f/11SJTU_r.png"  alt="right" />
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Constructed three Codon Switches that controls protein biosynthesis, Rare-Codon Switch, Stop-Codon Switch and Initial-Codon Switch. </p>
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Constructed three Codon Switches that controls protein biosynthesis, <a href="/Team:SJTU-BioX-Shanghai/Project/Subproject1">Rare-Codon Switch</a>, <a href="/Team:SJTU-BioX-Shanghai/Project/Subproject2">Stop-Codon Switch</a> and <a href="/Team:SJTU-BioX-Shanghai/Project/Subproject3">Initial-Codon Switch</a>. </p>
<p><img src="/wiki/images/7/7f/11SJTU_r.png"  alt="right" />
<p><img src="/wiki/images/7/7f/11SJTU_r.png"  alt="right" />
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Used Rare-Codon Switch to turn up/down protein biosynthesis by controlling rare tRNA amount, aaRS and the number of rare codons. </p>
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Used <a href="/Team:SJTU-BioX-Shanghai/Project/Subproject1">Rare-Codon Switch</a> to turn up/down protein biosynthesis by controlling <a href="/Team:SJTU-BioX-Shanghai/Project/Subproject1-1">rare tRNA amount</a>, <a href="/Team:SJTU-BioX-Shanghai/Project/Subproject1-2">aaRS</a> and <a href="/Team:SJTU-BioX-Shanghai/Project/Subproject1-3">the number of rare codons.</a> </p>
<p><img src="/wiki/images/7/7f/11SJTU_r.png"  alt="right" />
<p><img src="/wiki/images/7/7f/11SJTU_r.png"  alt="right" />
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Built our Rare-Codon Switch <i>in silic</i> and demonstrated the reliability of the model through our experiment results.  
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Built our Rare-Codon Switch <a href="/Team:SJTU-BioX-Shanghai/Project/Subproject1/Modeling"><i>in silic</i></a> and demonstrated the reliability of the model through our experiment results.  
</p>
</p>
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<p><img src="/wiki/images/7/7f/11SJTU_r.png"  alt="right" />Constructed two strict switches, Stop-Codon Switch and Initial-Codon Switch, that can turn on/off protein biosynthesis. </p>
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<p><img src="/wiki/images/7/7f/11SJTU_r.png"  alt="right" />
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Constructed two strict switches, <a href="/Team:SJTU-BioX-Shanghai/Project/Subproject2">Stop-Codon Switch</a> and <a href="/Team:SJTU-BioX-Shanghai/Project/Subproject3">Initial-Codon Switch</a>, that can turn on/off protein biosynthesis. </p>
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<p><img src="/wiki/images/7/7f/11SJTU_r.png"  alt="right" />
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Demonstrated <b>device application</b> by constructing and successfully testing <a href="/Team:SJTU-BioX-Shanghai/Project/Application">positive feedback</a> with our device in metabolic pathway. </p>
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<p><img src="/wiki/images/7/7f/11SJTU_r.png"  alt="right" />Constructed 21 biobricks in our 3 sub-projects  
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<p><img src="/wiki/images/7/7f/11SJTU_r.png"  alt="right" />
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Constructed <a href="/Team:SJTU-BioX-Shanghai/Parts">27 biobricks</a> in our 3 sub-projects.
</p>
</p>
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<p><img src="/wiki/images/7/7f/11SJTU_r.png"  alt="right" />Analysed the structures of 18 aaRS with structural biological methods. Designed structures of 14 modified aaRS deprived of their anticodon recognition ability.
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<p><img src="/wiki/images/7/7f/11SJTU_r.png"  alt="right" />
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Analysed the structures of 18 aaRS with <a href="/Team:SJTU-BioX-Shanghai/Project/Application-3">structural biological methods</a>. Designed structures of 14 modified aaRS deprived of their anticodon recognition ability.
</p>
</p>
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<p><img src="/wiki/images/7/7f/11SJTU_r.png"  alt="right" />Expanded the regulating tools for synthetic biology and developed two brand-new ways that facilitate the study of protein function.  
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<p><img src="/wiki/images/7/7f/11SJTU_r.png"  alt="right" />
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Expanded the <a href="/Team:SJTU-BioX-Shanghai/Project/Application">regulating tools</a> for synthetic biology and developed two brand-new ways that facilitate the study of protein function.  
</p>
</p>
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 +
<p><img src="/wiki/images/7/7f/11SJTU_r.png"  alt="right" />
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Designed our Human Practice Project: <a href="/Team:SJTU-BioX-Shanghai/Human_practice">T-PostCards</a> to promote our views on synthetic biology through communications and connections.
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</p>
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<p><img src="/wiki/images/7/7f/11SJTU_r.png"  alt="right" />
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Promoted synthetic biology <a href="/Team:SJTU-BioX-Shanghai/Human_practice_2">in high school class</a>. Designed a questionnaire and did a survey on high school students' understandings and attitudes towards synthetic biology.
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</p>
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<p><img src="/wiki/images/7/7f/11SJTU_r.png"  alt="right" />
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Attended the <b>2011 China Meet Up</b> and presented our project idea to other iGEMer and advisors. </p>
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Latest revision as of 03:52, 29 October 2011

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    The Project: Codon Switch Controlling Protein Biosynthesis

    SJTU-BioX-Shanghai iGEM team is designing a set of Codon-Switches that regulate target protein biosynthesis (translation).

    In our Rare-Codon Switch, the translation of the protein can be finely turned up/down with the control of rare tRNA amount, aaRS that charges the rare tRNA and rare codons.

    Besides, our device can be made into switches that can be turned on/off without background noise in two ways. One is to use stop codon as the controlling element, the Stop-Codon Switch. The other is to use any codon but the original start codon to initiate translation, the Initial-Codon Switch.

    Project

    Our design has expanded the regulating tools for synthetic biology and introduced brand-new methods for protein function analysis. See more about project applications, click here.

    Team
    Parts
    Human practice
    Achievements

    right Gold Medal and Best New BioBrick Part or Device, Engineered: BBa_K567011 & BBa_K567012 at Asia Jamboree. Advancing to World Champion!

    right Constructed three Codon Switches that controls protein biosynthesis, Rare-Codon Switch, Stop-Codon Switch and Initial-Codon Switch.

    right Used Rare-Codon Switch to turn up/down protein biosynthesis by controlling rare tRNA amount, aaRS and the number of rare codons.

    right Built our Rare-Codon Switch in silic and demonstrated the reliability of the model through our experiment results.

    right Constructed two strict switches, Stop-Codon Switch and Initial-Codon Switch, that can turn on/off protein biosynthesis.

    right Demonstrated device application by constructing and successfully testing positive feedback with our device in metabolic pathway.

    right Constructed 27 biobricks in our 3 sub-projects.

    right Analysed the structures of 18 aaRS with structural biological methods. Designed structures of 14 modified aaRS deprived of their anticodon recognition ability.

    right Expanded the regulating tools for synthetic biology and developed two brand-new ways that facilitate the study of protein function.

    right Designed our Human Practice Project: T-PostCards to promote our views on synthetic biology through communications and connections.

    right Promoted synthetic biology in high school class. Designed a questionnaire and did a survey on high school students' understandings and attitudes towards synthetic biology.

    right Attended the 2011 China Meet Up and presented our project idea to other iGEMer and advisors.

    Retrieved from "http://2011.igem.org/Team:SJTU-BioX-Shanghai"