Team:Fatih Turkey/Experiments5
From 2011.igem.org
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<td width="600" height="223" valign="top"><p><strong>Fenton Reagent Assay</strong><br /> | <td width="600" height="223" valign="top"><p><strong>Fenton Reagent Assay</strong><br /> | ||
- | + | <p><strong><img src="https://static.igem.org/mediawiki/2011/0/05/2011-07-15_18.08.22.jpg" width="500" /></strong></p> | |
+ | <p> To make our lab safe and secure; we needed to solve the safety problem caused by B.subtilis endospores. Endospores may gain pathogenicity according to the study done by Imperial College in 2008 iGEM. (<a href="http://partsregistry.org/Help:Bacillus_subtilis/Advantages_and_disadvantages">http://partsregistry.org/Help:Bacillus_subtilis/Advantages_and_disadvantages</a>) <br /> | ||
In order to avoid such dangerous circumstances, we used a chemical solution that kills both original bacterium forms and endospores effectively: Fenton Reagent. The solution decontaminates the media with help of production of oxygen radicals. These radicals damage the DNA content and enzymes in the bacterium that are needed to reform original bacterium body. <br /> | In order to avoid such dangerous circumstances, we used a chemical solution that kills both original bacterium forms and endospores effectively: Fenton Reagent. The solution decontaminates the media with help of production of oxygen radicals. These radicals damage the DNA content and enzymes in the bacterium that are needed to reform original bacterium body. <br /> | ||
We prepared modified Fenton Reagnet solution for our purpose according to J. B. Cross , 2003.1 Normally, Fenton Reagent produces oxygen radicals by the help of hydrogen peroxide. On the other hand, modified version includes copper ions and dissolved oxygen which boosts killing mechanism. We prepared this solution in two ways: With distilled water (DFR) or oxidized water. (OFR)<br /> | We prepared modified Fenton Reagnet solution for our purpose according to J. B. Cross , 2003.1 Normally, Fenton Reagent produces oxygen radicals by the help of hydrogen peroxide. On the other hand, modified version includes copper ions and dissolved oxygen which boosts killing mechanism. We prepared this solution in two ways: With distilled water (DFR) or oxidized water. (OFR)<br /> |
Latest revision as of 20:02, 28 October 2011
Fenton Reagent Assay
To make our lab safe and secure; we needed to solve the safety problem caused by B.subtilis endospores. Endospores may gain pathogenicity according to the study done by Imperial College in 2008 iGEM. (http://partsregistry.org/Help:Bacillus_subtilis/Advantages_and_disadvantages) |