Team:SJTU-BioX-Shanghai

From 2011.igem.org

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<div class="title"><b>The Project: Codon Switch Controlling Protein Biosynthesis</b></div>
<div class="title"><b>The Project: Codon Switch Controlling Protein Biosynthesis</b></div>
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<div class="title"><b>Achievements</b></div>
<div class="title"><b>Achievements</b></div>
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<img src="/wiki/images/3/3a/11SJTU_cheering.jpg" style="float:right;margin:10px;">
<p><img src="/wiki/images/7/7f/11SJTU_r.png"  alt="right" />
<p><img src="/wiki/images/7/7f/11SJTU_r.png"  alt="right" />
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Developed tRNA and aminoacyl tRNA synthetase (aaRS) from the natural biological system into the <a href="/Team:SJTU-BioX-Shanghai/Parts/Data_Page"> Modulator, a controlling device for protein biosynthesis</a>. The function of this device has been fully characterized. Brand-new features of the device are also discovered.</p>
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<b>Gold Medal</b> and <b>Best New BioBrick Part or Device, Engineered</b>: <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K567011">BBa_K567011</a> & <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K567012">BBa_K567012</a> at Asia Jamboree. Advancing to World Champion!</p>
<p><img src="/wiki/images/7/7f/11SJTU_r.png"  alt="right" />
<p><img src="/wiki/images/7/7f/11SJTU_r.png"  alt="right" />
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Constructed 4 <a href="/Team:SJTU-BioX-Shanghai/Parts">Modulators</a> (including 8 biobricks) and 13 <a href="/Team:SJTU-BioX-Shanghai/Parts">Reporter</a> biobricks in our 3 subprojedts to test our controlling system. Create parts, including our favorite parts have been sent to Registry.</p>
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Constructed three Codon Switches that controls protein biosynthesis, <a href="/Team:SJTU-BioX-Shanghai/Project/Subproject1">Rare-Codon Switch</a>, <a href="/Team:SJTU-BioX-Shanghai/Project/Subproject2">Stop-Codon Switch</a> and <a href="/Team:SJTU-BioX-Shanghai/Project/Subproject3">Initial-Codon Switch</a>. </p>
<p><img src="/wiki/images/7/7f/11SJTU_r.png"  alt="right" />
<p><img src="/wiki/images/7/7f/11SJTU_r.png"  alt="right" />
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Modified pre-existing reporter genes <a href="/Team:SJTU-BioX-Shanghai/Parts">luciferase</a> and <a href="/Team:SJTU-BioX-Shanghai/Parts">RFP</a> and created a series of new reporter genes. These reporters have been excellent devices in characterizing our system. </p>
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Used <a href="/Team:SJTU-BioX-Shanghai/Project/Subproject1">Rare-Codon Switch</a> to turn up/down protein biosynthesis by controlling <a href="/Team:SJTU-BioX-Shanghai/Project/Subproject1-1">rare tRNA amount</a>, <a href="/Team:SJTU-BioX-Shanghai/Project/Subproject1-2">aaRS</a> and <a href="/Team:SJTU-BioX-Shanghai/Project/Subproject1-3">the number of rare codons.</a> </p>
<p><img src="/wiki/images/7/7f/11SJTU_r.png"  alt="right" />
<p><img src="/wiki/images/7/7f/11SJTU_r.png"  alt="right" />
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Built our Rare-Codon Switch <a href="/Team:SJTU-BioX-Shanghai/Project/Subproject1/Modeling" ><i>in silic</i></a> and demonstrated <b>the reliability of the model</b>through our experiment results. The data in silic and data experimental matches well.</p>
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Built our Rare-Codon Switch <a href="/Team:SJTU-BioX-Shanghai/Project/Subproject1/Modeling"><i>in silic</i></a> and demonstrated the reliability of the model through our experiment results.  
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</p>
<p><img src="/wiki/images/7/7f/11SJTU_r.png"  alt="right" />
<p><img src="/wiki/images/7/7f/11SJTU_r.png"  alt="right" />
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Analyzed the structure of 18 aaRS with <a href="/Team:SJTU-BioX-Shanghai/Project/Subproject1/Modeling_2">structural biological methods</a>. Designed structures of 14 modified aaRS deprived of their anticodon recognition ability. This work can be used to expand our device aaRS Modulator. </p>
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Constructed two strict switches, <a href="/Team:SJTU-BioX-Shanghai/Project/Subproject2">Stop-Codon Switch</a> and <a href="/Team:SJTU-BioX-Shanghai/Project/Subproject3">Initial-Codon Switch</a>, that can turn on/off protein biosynthesis. </p>
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<p><img src="/wiki/images/7/7f/11SJTU_r.png"  alt="right" />
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Demonstrated <b>device application</b> by constructing and successfully testing <a href="/Team:SJTU-BioX-Shanghai/Project/Application">positive feedback</a> with our device in metabolic pathway. </p>
<p><img src="/wiki/images/7/7f/11SJTU_r.png"  alt="right" />
<p><img src="/wiki/images/7/7f/11SJTU_r.png"  alt="right" />
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Designed two different <a href="/Team:SJTU-BioX-Shanghai/Project/Application">models</a> for how our device can be applied to <b>controlling metabolic networks</b>, which expands the regulating tools for synthetic biology. </p>
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Constructed <a href="/Team:SJTU-BioX-Shanghai/Parts">27 biobricks</a> in our 3 sub-projects.
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</p>
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<p><img src="/wiki/images/7/7f/11SJTU_r.png"  alt="right" />
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Analysed the structures of 18 aaRS with <a href="/Team:SJTU-BioX-Shanghai/Project/Application-3">structural biological methods</a>. Designed structures of 14 modified aaRS deprived of their anticodon recognition ability.
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</p>
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<p><img src="/wiki/images/7/7f/11SJTU_r.png"  alt="right" />
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Expanded the <a href="/Team:SJTU-BioX-Shanghai/Project/Application">regulating tools</a> for synthetic biology and developed two brand-new ways that facilitate the study of protein function.  
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</p>
<p><img src="/wiki/images/7/7f/11SJTU_r.png"  alt="right" />
<p><img src="/wiki/images/7/7f/11SJTU_r.png"  alt="right" />
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Developed two brand-new ways that <a href="/Team:SJTU-BioX-Shanghai/Project/Application">facilitate the study of protein structure</a>. One for incorporating point mutation into protein. The other for producing truncated proteins to help study the important protein domains. </p>
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Designed our Human Practice Project: <a href="/Team:SJTU-BioX-Shanghai/Human_practice">T-PostCards</a> to promote our views on synthetic biology through communications and connections.  
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</p>
<p><img src="/wiki/images/7/7f/11SJTU_r.png"  alt="right" />
<p><img src="/wiki/images/7/7f/11SJTU_r.png"  alt="right" />
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Design our Human Practice Project: <a href="/Team:SJTU-BioX-Shanghai/Human_practice">T-PostCards</a> to promote our views on synthetic biology through communications and connections.  
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Promoted synthetic biology <a href="/Team:SJTU-BioX-Shanghai/Human_practice_2">in high school class</a>. Designed a questionnaire and did a survey on high school students' understandings and attitudes towards synthetic biology.  
</p>
</p>
<p><img src="/wiki/images/7/7f/11SJTU_r.png"  alt="right" />
<p><img src="/wiki/images/7/7f/11SJTU_r.png"  alt="right" />

Latest revision as of 03:52, 29 October 2011

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    The Project: Codon Switch Controlling Protein Biosynthesis

    SJTU-BioX-Shanghai iGEM team is designing a set of Codon-Switches that regulate target protein biosynthesis (translation).

    In our Rare-Codon Switch, the translation of the protein can be finely turned up/down with the control of rare tRNA amount, aaRS that charges the rare tRNA and rare codons.

    Besides, our device can be made into switches that can be turned on/off without background noise in two ways. One is to use stop codon as the controlling element, the Stop-Codon Switch. The other is to use any codon but the original start codon to initiate translation, the Initial-Codon Switch.

    Project

    Our design has expanded the regulating tools for synthetic biology and introduced brand-new methods for protein function analysis. See more about project applications, click here.

    Team
    Parts
    Human practice
    Achievements

    right Gold Medal and Best New BioBrick Part or Device, Engineered: BBa_K567011 & BBa_K567012 at Asia Jamboree. Advancing to World Champion!

    right Constructed three Codon Switches that controls protein biosynthesis, Rare-Codon Switch, Stop-Codon Switch and Initial-Codon Switch.

    right Used Rare-Codon Switch to turn up/down protein biosynthesis by controlling rare tRNA amount, aaRS and the number of rare codons.

    right Built our Rare-Codon Switch in silic and demonstrated the reliability of the model through our experiment results.

    right Constructed two strict switches, Stop-Codon Switch and Initial-Codon Switch, that can turn on/off protein biosynthesis.

    right Demonstrated device application by constructing and successfully testing positive feedback with our device in metabolic pathway.

    right Constructed 27 biobricks in our 3 sub-projects.

    right Analysed the structures of 18 aaRS with structural biological methods. Designed structures of 14 modified aaRS deprived of their anticodon recognition ability.

    right Expanded the regulating tools for synthetic biology and developed two brand-new ways that facilitate the study of protein function.

    right Designed our Human Practice Project: T-PostCards to promote our views on synthetic biology through communications and connections.

    right Promoted synthetic biology in high school class. Designed a questionnaire and did a survey on high school students' understandings and attitudes towards synthetic biology.

    right Attended the 2011 China Meet Up and presented our project idea to other iGEMer and advisors.

    Retrieved from "http://2011.igem.org/Team:SJTU-BioX-Shanghai"