Team:SJTU-BioX-Shanghai/Parts/Data Page

From 2011.igem.org

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===Rare-Codon Switch: control with rare tRNA amount===
===Rare-Codon Switch: control with rare tRNA amount===
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[[image:11SJTU_rare_01.jpg|center]]
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*'''Data for our favorite new parts'''
*'''Data for our favorite new parts'''
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*'''Data for Pre-existing Parts'''
*'''Data for Pre-existing Parts'''
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#[http://partsregistry.org/Part:BBa_I712019:Experience Experience] - BBa_I712019 Firefly luciferase (Anja Korenčič 2007)
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#[http://partsregistry.org/Part:BBa_I712019:Experience Experience] - BBa_I712019 Firefly luciferase (Anja Korenčič 2007)
*'''We've Also Characterized the Following Part'''
*'''We've Also Characterized the Following Part'''
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===Rare-Codon Switch: control with aaRS===
===Rare-Codon Switch: control with aaRS===
 +
 +
[[image:11SJTU_rare_03.jpg|center]]
*'''Data for our favorite new parts'''
*'''Data for our favorite new parts'''
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*[[Team:SJTU-BioX-Shanghai/Project/Subproject1-2|See Project Design and Result]]
*[[Team:SJTU-BioX-Shanghai/Project/Subproject1-2|See Project Design and Result]]
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*[[Team:SJTU-BioX-Shanghai/Project/Subproject1/Modeling_2|See Project Modeling]]  
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*[[Team:SJTU-BioX-Shanghai/Project/Application-3|See Project Modeling]]  
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===Rare-Codon Switch: controlling rare codon number===
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===Rare-Codon Switch: control with rare codon number, locations and tandem copies===
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[[image:11SJTU rare 12.jpg|center]]
*'''Data for our favorite new parts'''
*'''Data for our favorite new parts'''
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#[http://partsregistry.org/wiki/index.php?title=Part:BBa_K567008 Main Page] - PT7/P''bla''-Luc-nAGG
 
#[http://partsregistry.org/wiki/index.php?title=Part:BBa_K567001 Main Page] - BBa_K567001 ''lacI'' -Ptrc-tRNA<sup>Arg</sup>: The tRNA<sup>Arg</sup>(drived from ''E.coli ArgW'' operon) is under the control of trc promoter, induced by IPTG.
#[http://partsregistry.org/wiki/index.php?title=Part:BBa_K567001 Main Page] - BBa_K567001 ''lacI'' -Ptrc-tRNA<sup>Arg</sup>: The tRNA<sup>Arg</sup>(drived from ''E.coli ArgW'' operon) is under the control of trc promoter, induced by IPTG.
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#[http://partsregistry.org/wiki/index.php?title=Part:BBa_K567004 Main Page] - PT7/P''bla''-Luc-nAGG  Date for ''control with rare codon number''
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#[http://partsregistry.org/wiki/index.php?title=Part:BBa_K567021 Main Page] -PT7-Luc-2x4AGG(111bp)  Date for ''control with rare codon number, locations and tandem copies''
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==Stop-Codon Switch==
==Stop-Codon Switch==
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[[image:11SJTU_stop_01.jpg|center]]
*'''Data for our favorite new parts'''
*'''Data for our favorite new parts'''
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*[[Team:SJTU-BioX-Shanghai/Project/Subproject3|See Project Design and Result]]
*[[Team:SJTU-BioX-Shanghai/Project/Subproject3|See Project Design and Result]]
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 +
-----
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==Positive FeedBack==
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*'''Data for our new parts'''
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# [http://partsregistry.org/wiki/index.php?title=Part:BBa_K567022 Main Page] - PT7-Luc-nAGG-tRNA(Arg). This biobrick is under the control of T7 promoter and'' lac'' operator.
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*[[Team:SJTU-BioX-Shanghai/Project/Application|See this Application]]

Latest revision as of 03:57, 29 October 2011

    •


    Data Page

    Rare-Codon Switch

    Rare-Codon Switch: control with rare tRNA amount

    11SJTU rare 01.jpg
    • Data for our favorite new parts
    1. [http://partsregistry.org/wiki/index.php?title=Part:BBa_K567001 Main Page] - BBa_K567001 lacI -Ptrc-tRNAArg: The tRNAArg(drived from E.coli ArgW operon) is under the control of trc promoter, induced by IPTG.
    • Data for Pre-existing Parts
    1. [http://partsregistry.org/Part:BBa_I712019:Experience Experience] - BBa_I712019 Firefly luciferase (Anja Korenčič 2007)
    • We've Also Characterized the Following Part
    1. [http://partsregistry.org/wiki/index.php?title=Part:BBa_K567002 Main Page] - BBa_K567002 sulA promoter-tRNAArg :The tRNAArg is under the control of sulA promoter induced by UV.



    Rare-Codon Switch: control with aaRS

    11SJTU rare 03.jpg
    • Data for our favorite new parts
    1. [http://partsregistry.org/wiki/index.php?title=Part:BBa_K567011 Main Page] - BBa_K567011 PT7-TDRS :Aspartyl aminoacyl tRNA synthetase without anticodon recognition domain under the control of T7 promoter and lac operator.
    2. [http://partsregistry.org/wiki/index.php?title=Part:BBa_K567012 Main Page] - BBa_K567012 tRNAAsp-AGG : The tRNAAsp-AGG with its anticodon mutated to CCU(base pairing rare codon AGG) and under the control of aspV promoter.
    • Data for Pre-existing Parts
    1. [http://partsregistry.org/Part:BBa_I13521:Experience Experience] - BBa_I13521 Ptet mRFP (Endy Lab 2005)
    • We've Also Characterized the Following Part
    1. [http://partsregistry.org/wiki/index.php?title=Part:BBa_K567017 Main Page] - BBa_K567017 PT7-RFP-6AGG



    Rare-Codon Switch: control with rare codon number, locations and tandem copies

    11SJTU rare 12.jpg
    • Data for our favorite new parts
    1. [http://partsregistry.org/wiki/index.php?title=Part:BBa_K567001 Main Page] - BBa_K567001 lacI -Ptrc-tRNAArg: The tRNAArg(drived from E.coli ArgW operon) is under the control of trc promoter, induced by IPTG.
    2. [http://partsregistry.org/wiki/index.php?title=Part:BBa_K567004 Main Page] - PT7/Pbla-Luc-nAGG Date for control with rare codon number
    3. [http://partsregistry.org/wiki/index.php?title=Part:BBa_K567021 Main Page] -PT7-Luc-2x4AGG(111bp) Date for control with rare codon number, locations and tandem copies



    Stop-Codon Switch

    11SJTU stop 01.jpg
    • Data for our favorite new parts
    1. [http://partsregistry.org/wiki/index.php?title=Part:BBa_K567011 Main Page] - BBa_K567011 PT7-TDRS :Aspartyl aminoacyl tRNA synthetase without anticodon recognition domain under the control of T7 promoter and lac operator.
    2. [http://partsregistry.org/wiki/index.php?title=Part:BBa_K567013 Main Page] - BBa_K567013 tRNAAsp-TAG : The tRNAAsp-TAG with its anticodon mutated to CUA (base pairing stop codon UAG) and under the control of aspV promoter.
    • We've Also Characterized the Following Part
    1. [http://partsregistry.org/wiki/index.php?title=Part:BBa_K567003 Main Page] - BBa_K567003 Pbla-Luc-TAG
    • We've Also Made the Following Part
    1. [http://partsregistry.org/wiki/index.php?title=Part:BBa_K567018 Main Page] - BBa_K567018 PT7-GFP-TAG-RFP



    Initial-Codon Switch

    • Data for our favorite new parts
    1. [http://partsregistry.org/wiki/index.php?title=Part:BBa_K567014 Main Page] - BBa_K567014 PT7-metGM : T7 promoter-metG(mutated). This biobrick is constructed by putting the mutated metG (Met-RS) under the control of T7 promoter and lac operator.
    2. [http://partsregistry.org/wiki/index.php?title=Part:BBa_K567015 Main Page] - BBa_K567015 PT7-metGN :T7 promoter-metG(truncated). This biobrick is constructed by putting the truncated metG (Met-RS) under the control of T7 promoter and lac operator.
    3. [http://partsregistry.org/wiki/index.php?title=Part:BBa_K567016 Main Page] - BBa_K567016 metY-CGA :This biobrick is constructed by mutating the anticodon of tRNAmet to TCG (base pairing codon CGA).
    • We've Also Characterized the Following Part
    1. [http://partsregistry.org/wiki/index.php?title=Part:BBa_K567020 Main Page] - BBa_K567020 KanR-CGA


    Positive FeedBack

    • Data for our new parts
    1. [http://partsregistry.org/wiki/index.php?title=Part:BBa_K567022 Main Page] - PT7-Luc-nAGG-tRNA(Arg). This biobrick is under the control of T7 promoter and lac operator.
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