Team:Fatih Turkey/Mustafa

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<a href="https://2011.igem.org/Team:Fatih_Turkey/Project">Project<span>Rainbow Graveyard</span></a>

<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Results">Results</a></li>

<a href="https://2011.igem.org/Team:Fatih_Turkey/Biobricks">Biobricks</a>

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<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Parts">Parts</a></li>

<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Devices">Devices</a></li>

<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Data_Page">Data Page</a></li>

<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Safety">Safety</a></li>

<li><a href="https://2011.igem.org/Team:Fatih_Turkey/Sporocide">Sporocide</a></li>

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<h1>FATIH UNIVERSITY MEDICAL SCHOOL

ANSWERS TO NEW SAFETY AND SECURITY QUESTIONS</h1>

<b>1. Would the materials used in your project and/or your final product pose:</b><br>

<b>a. Risks to the safety and health of team members or others in the lab?</b><br>

<p style="font-family: Verdana, Arial, SunSans-Regular, sans-serif;font-size:12px;">Actually few hazardous chemicals and solutions are used in some lab procedures such as gel preparation and electrophoresis. However these chemicals and solutions are used according to the safety rules of the laboratory with care and caution. All the members were trained for safety regulations of the laboratory as well as toxicity of the chemicals and solutions before starting the current project.</p>

<b>b. Risks to the safety and health of the general public if released by design or accident?</b><br>

<p style="font-family: Verdana, Arial, SunSans-Regular, sans-serif;font-size:12px;">When released by accident, our parts and materials actually cause no negative damage to the general public. B.subtilis is known not to be associated with disease process in humans in regular conditions and thus are supposed to be benign by nature and present no danger to anyone. Only under stress conditions, B. subtilis may form spores and these spores are resistant versions of single cells. While B. subtilis itself isn't pathogenic, its spores may be infectious if inhaled. But all of our team members are trained for safety of the laboratory rules and use mask and gloves when dealing with bacteria. Besides all experiments are performed in synthetic laboratory designated by Fatih University Medical School and no material is carried out of the laboratory. However to increase public safety of our project, in case of accident, our plan is to destroy particles including B.subtilis by cleaning the biofilm from the spores and treating with Fenton reagent containing dissolved oxygen and other constituents.</p>

<b>c. Risks to environmental quality if released by design or accident?</b><br>

<p style="font-family: Verdana, Arial, SunSans-Regular, sans-serif;font-size:12px;">Bacillus subtilis strain 3610 is a wild type strain that is already widespread in the environment. B. subtilis strain 168  is a derivative strain of the 3610 and has disabling auxotrophs mutations  that makes it even less likely to colonize or cause harm to human or animal health. Our experiment does not include any modifications, which enhance their ability to survive or disseminate. Thus, there is no specific environmental risk associated with use of the Bacillus subtilis strains except its spore formation. In case of accident, cleaning and treating the spores with Fenton reagent as mentioned above provide environmental safety. Besides all procedures are performed in a specified laboratory.</p>

<p style="font-family: Verdana, Arial, SunSans-Regular, sans-serif;font-size:12px;">E. Coli strains TOP10 and BL1 have very limited ability to survive outside the laboratory so that it would be unable to survive or disseminate. Therefore, there is no specific environmental risk associated with the E. coli strains.  

All bacterial waste are kept in 10% bleaching solution for one day, then are autoclaved to be sterilized.

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<b>d. Risks to security through malicious misuse by individuals, groups or states?</b><br>

<p style="font-family: Verdana, Arial, SunSans-Regular, sans-serif;font-size:12px;">Please explain your responses (whether yes or no) to these questions. Specifically, are any parts or devices in your project associated with (or known to cause):</p>

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<li>pathogenicity, infectivity, or toxicity? No </li>

<li>threats to environmental quality? No</li>

<li>security concerns? No</li>

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<p style="font-family: Verdana, Arial, SunSans-Regular, sans-serif;font-size:12px;">As explained below:<br>

Nothing could be used maliciously in our project. The materials we use are commonly used in experimental procedures in various laboratories all over the world and all are harmless and do not pose any threat to environment and public as well as security concerns.</p>

 

 

 

 

 

 

 

 

 

 

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