Team:SJTU-BioX-Shanghai/Parts/Data Page

From 2011.igem.org

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==Rare-Codon Switch==
==Rare-Codon Switch==
-
===Rare-Codon Switch: controlling rare tRNA amount===
+
===Rare-Codon Switch: control with rare tRNA amount===
 +
 
 +
[[image:11SJTU_rare_01.jpg|center]]
 +
 
*'''Data for our favorite new parts'''
*'''Data for our favorite new parts'''
-
#[http://partsregistry.org/wiki/index.php?title=Part:BBa_K567001 Main Page] - BBa_K567001 lacI -Ptrc-tRNAArg: The tRNAArg(drived from E.coli ArgW operon) is under the control of trc promoter, induced by IPTG.
+
#[http://partsregistry.org/wiki/index.php?title=Part:BBa_K567001 Main Page] - BBa_K567001 ''lacI'' -Ptrc-tRNA<sup>Arg</sup>: The tRNA<sup>Arg</sup>(drived from ''E.coli ArgW'' operon) is under the control of trc promoter, induced by IPTG.
*'''Data for Pre-existing Parts'''
*'''Data for Pre-existing Parts'''
-
#[http://partsregistry.org/Part:BBa_I712019:Experience Experience] - BBa_I712019 Firefly luciferase (Anja Korenčič 2007)
+
#[http://partsregistry.org/Part:BBa_I712019:Experience Experience] - BBa_I712019 Firefly luciferase (Anja Korenčič 2007)
*'''We've Also Characterized the Following Part'''
*'''We've Also Characterized the Following Part'''
-
#[http://partsregistry.org/wiki/index.php?title=Part:BBa_K5670002 Main Page] - BBa_K567002 sulA promoter-tRNAArg :The tRNAArg is under the control of sulApromoter induced by UV.
+
#[http://partsregistry.org/wiki/index.php?title=Part:BBa_K567002 Main Page] - BBa_K567002 ''sulA'' promoter-tRNA<sup>Arg</sup> :The tRNA<sup>Arg</sup> is under the control of ''sulA'' promoter induced by UV.
-
*[[Team:SJTU-BioX-Shanghai/Project/Subproject1/Design|See Project Design]]
+
*[[Team:SJTU-BioX-Shanghai/Project/Subproject1-1|See Project Design and Result]]
-
*[[Team:SJTU-BioX-Shanghai/Project/Subproject1/Modeling_2|See Project Modeling]]
+
 
-
*[[Team:SJTU-BioX-Shanghai/Project/Subproject1/Results_3|See Project Result]]
+
----------
----------
-
===Rare-Codon Switch: tRNA Modulator + Quantitative Reporter===
+
===Rare-Codon Switch: control with aaRS===
-
[[image:11SJTU-data_page-tRNA%2Bluc.jpg|500px|center]]
+
 
 +
[[image:11SJTU_rare_03.jpg|center]]
*'''Data for our favorite new parts'''
*'''Data for our favorite new parts'''
-
#[http://partsregistry.org/wiki/index.php?title=Part:BBa_K567001 Main Page] - BBa_K567001 ''lacI'' -Ptrc-tRNA<sup>Arg</sup> :The tRNA<sup>Arg</sup>(drived from ''E.coli'' ''ArgW'' operon) is under the control of trc promoter, induced by IPTG.
+
#[http://partsregistry.org/wiki/index.php?title=Part:BBa_K567011 Main Page] - BBa_K567011 PT7-TDRS :Aspartyl aminoacyl tRNA synthetase without anticodon recognition domain under the control of T7 promoter and ''lac'' operator.
 +
#[http://partsregistry.org/wiki/index.php?title=Part:BBa_K567012 Main Page] - BBa_K567012 tRNA<sup>Asp</sup>-AGG : The tRNA<sup>Asp</sup>-AGG with its anticodon mutated to CCU(base pairing rare codon AGG) and under the control of ''aspV'' promoter.
*'''Data for Pre-existing Parts'''
*'''Data for Pre-existing Parts'''
-
#[http://partsregistry.org/Part:BBa_I712019:Experience Experience] - BBa_I712019 Firefly luciferase (Anja Korenčič 2007)
+
#[http://partsregistry.org/Part:BBa_I13521:Experience Experience] - BBa_I13521 Ptet mRFP (Endy Lab 2005)
*'''We've Also Characterized the Following Part'''
*'''We've Also Characterized the Following Part'''
 +
#[http://partsregistry.org/wiki/index.php?title=Part:BBa_K567017 Main Page] - BBa_K567017 PT7-RFP-6AGG
-
1. PT7/P''bla''-Luc-nAGG
 
-
If you want to know more about PT7/P''bla''-Luc-nAGG and ''lacI''-Ptrc-tRNA<sup>Arg</sup>, click [http://partsregistry.org/wiki/index.php?title=Part:BBa_K567001 here]  
+
*[[Team:SJTU-BioX-Shanghai/Project/Subproject1-2|See Project Design and Result]]
 +
*[[Team:SJTU-BioX-Shanghai/Project/Application-3|See Project Modeling]]  
-
If you want to know more about PT7/P''bla''-Luc-nAGG and PT7-TDRS, click [http://partsregistry.org/wiki/index.php?title=Part:BBa_K567011 here]
 
-
 
-
2. [http://partsregistry.org/wiki/index.php?title=Part:BBa_K567002 Main Page] - BBa_K567002 ''sulA'' promoter-tRNA<sup>Arg</sup> :The tRNA<sup>Arg</sup> is under the control of ''sulA'' promoter induced by UV.
 
-
 
-
 
-
 
-
*[[Team:SJTU-BioX-Shanghai/Project/Subproject1/Design|See Project Design]]
 
-
*[[Team:SJTU-BioX-Shanghai/Project/Subproject1/Modeling|See Project Modeling]]
 
-
*[[Team:SJTU-BioX-Shanghai/Project/Subproject1/Results_2|See Project Result]]
 
-----
-----
-
===Rare-Codon Switch: aaRS Modulator + Quantitative Reporter===
+
===Rare-Codon Switch: control with rare codon number, locations and tandem copies===
-
This system is consisted of aaRS Modulator and Quantitative Reporter, which have been characterized separately above. The principle of this design is the same as the above two designs but showing a new combination.  
+
[[image:11SJTU rare 12.jpg|center]]
-
[[image:11SJTU-data_page-aaRS%2Bluc.jpg|500px|center]]
+
*'''Data for our favorite new parts'''
 +
#[http://partsregistry.org/wiki/index.php?title=Part:BBa_K567001 Main Page] - BBa_K567001 ''lacI'' -Ptrc-tRNA<sup>Arg</sup>: The tRNA<sup>Arg</sup>(drived from ''E.coli ArgW'' operon) is under the control of trc promoter, induced by IPTG.
 +
#[http://partsregistry.org/wiki/index.php?title=Part:BBa_K567004 Main Page] - PT7/P''bla''-Luc-nAGG  Date for ''control with rare codon number''
 +
#[http://partsregistry.org/wiki/index.php?title=Part:BBa_K567021 Main Page] -PT7-Luc-2x4AGG(111bp)  Date for ''control with rare codon number, locations and tandem copies''
-
*[[Team:SJTU-BioX-Shanghai/Project/Subproject1/Design|See Project Design]]
+
*[[Team:SJTU-BioX-Shanghai/Project/Subproject1-3|See Project Design and Result]]
-
*[[Team:SJTU-BioX-Shanghai/Project/Subproject1/Results_4|See Project Result]]
+
*[[Team:SJTU-BioX-Shanghai/Project/Subproject1/Modeling|See Project Modeling]]  
-
===Rare-Codon Switch: tRNA Modulator + Qualitative Reporter===
 
-
This system is consisted of tRNA Modulator and Qualitative Reporter, which have been characterized separately above. The principle of this design is the same as the above three designs but showing a new combination.
 
-
[[image:11SJTU-data_page-tRNA+RFP.jpg|500px|center]]
+
-----
-
 
+
-
 
+
-
 
+
-
*[[Team:SJTU-BioX-Shanghai/Project/Subproject1/Design|See Project Design]]
+
-
*[[Team:SJTU-BioX-Shanghai/Project/Subproject1/Results_1|See Project Result]]
+
-
 
+
-
 
+
-
 
+
-
----
+
==Stop-Codon Switch==
==Stop-Codon Switch==
-
===Stop-Codon Switch + Quantitative Reporter===
+
[[image:11SJTU_stop_01.jpg|center]]
-
 
+
-
[[image:11SJTU-data_page-stop%2Bluc.jpg|500px|center]]
+
*'''Data for our favorite new parts'''
*'''Data for our favorite new parts'''
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#[http://partsregistry.org/wiki/index.php?title=Part:BBa_K567003 Main Page] - BBa_K567003  P''bla''-Luc-TAG  
#[http://partsregistry.org/wiki/index.php?title=Part:BBa_K567003 Main Page] - BBa_K567003  P''bla''-Luc-TAG  
 +
*'''We've Also Made the Following Part'''
 +
#[http://partsregistry.org/wiki/index.php?title=Part:BBa_K567018 Main Page] - BBa_K567018  PT7-GFP-TAG-RFP
-
*[[Team:SJTU-BioX-Shanghai/Project/Subproject2#Design|See Project Design]]
+
*[[Team:SJTU-BioX-Shanghai/Project/Subproject2|See Project Design and Result]]
-
*[[Team:SJTU-BioX-Shanghai/Project/Subproject2#Results|See Project Result]]
+
-
===Stop-Codon Switch + Qualitative Reporter===
 
-
[[image:11SJTU-data_page-stop%2BGFPRFP.jpg|500px|center]]
 
-
 
+
-----
-
*'''We've Also Made the Following Part'''
+
-
#[http://partsregistry.org/wiki/index.php?title=Part:BBa_K567018 Main Page] - PT7-GFP-TAG-RFP
+
-
 
+
-
 
+
-
*[[Team:SJTU-BioX-Shanghai/Project/Subproject2#Design|See Project Design]]
+
-
*[[Team:SJTU-BioX-Shanghai/Project/Subproject2#Results|See Project Result]]
+
==Initial-Codon Switch==
==Initial-Codon Switch==
-
 
-
[[image:11SJTU-data_page-initial.jpg|500px|center]]
 
*'''Data for our favorite new parts'''
*'''Data for our favorite new parts'''
Line 124: Line 101:
*'''We've Also Characterized the Following Part'''
*'''We've Also Characterized the Following Part'''
-
# [http://partsregistry.org/wiki/index.php?title=Part:BBa_K567020 Main Page] - BBa_K567020  KanaR-CGA
+
# [http://partsregistry.org/wiki/index.php?title=Part:BBa_K567020 Main Page] - BBa_K567020  KanR-CGA
-
*[[Team:SJTU-BioX-Shanghai/Project/Subproject3#Design|See Project Design]]
+
*[[Team:SJTU-BioX-Shanghai/Project/Subproject3|See Project Design and Result]]
-
*[[Team:SJTU-BioX-Shanghai/Project/Subproject3#Results|See Project Result]]
+
 
 +
-----
 +
 
 +
==Positive FeedBack==
 +
*'''Data for our new parts'''
 +
# [http://partsregistry.org/wiki/index.php?title=Part:BBa_K567022 Main Page] - PT7-Luc-nAGG-tRNA(Arg). This biobrick is under the control of T7 promoter and'' lac'' operator.
 +
 
 +
*[[Team:SJTU-BioX-Shanghai/Project/Application|See this Application]]

Latest revision as of 03:57, 29 October 2011

    •


    Data Page

    Rare-Codon Switch

    Rare-Codon Switch: control with rare tRNA amount

    11SJTU rare 01.jpg
    • Data for our favorite new parts
    1. [http://partsregistry.org/wiki/index.php?title=Part:BBa_K567001 Main Page] - BBa_K567001 lacI -Ptrc-tRNAArg: The tRNAArg(drived from E.coli ArgW operon) is under the control of trc promoter, induced by IPTG.
    • Data for Pre-existing Parts
    1. [http://partsregistry.org/Part:BBa_I712019:Experience Experience] - BBa_I712019 Firefly luciferase (Anja Korenčič 2007)
    • We've Also Characterized the Following Part
    1. [http://partsregistry.org/wiki/index.php?title=Part:BBa_K567002 Main Page] - BBa_K567002 sulA promoter-tRNAArg :The tRNAArg is under the control of sulA promoter induced by UV.



    Rare-Codon Switch: control with aaRS

    11SJTU rare 03.jpg
    • Data for our favorite new parts
    1. [http://partsregistry.org/wiki/index.php?title=Part:BBa_K567011 Main Page] - BBa_K567011 PT7-TDRS :Aspartyl aminoacyl tRNA synthetase without anticodon recognition domain under the control of T7 promoter and lac operator.
    2. [http://partsregistry.org/wiki/index.php?title=Part:BBa_K567012 Main Page] - BBa_K567012 tRNAAsp-AGG : The tRNAAsp-AGG with its anticodon mutated to CCU(base pairing rare codon AGG) and under the control of aspV promoter.
    • Data for Pre-existing Parts
    1. [http://partsregistry.org/Part:BBa_I13521:Experience Experience] - BBa_I13521 Ptet mRFP (Endy Lab 2005)
    • We've Also Characterized the Following Part
    1. [http://partsregistry.org/wiki/index.php?title=Part:BBa_K567017 Main Page] - BBa_K567017 PT7-RFP-6AGG



    Rare-Codon Switch: control with rare codon number, locations and tandem copies

    11SJTU rare 12.jpg
    • Data for our favorite new parts
    1. [http://partsregistry.org/wiki/index.php?title=Part:BBa_K567001 Main Page] - BBa_K567001 lacI -Ptrc-tRNAArg: The tRNAArg(drived from E.coli ArgW operon) is under the control of trc promoter, induced by IPTG.
    2. [http://partsregistry.org/wiki/index.php?title=Part:BBa_K567004 Main Page] - PT7/Pbla-Luc-nAGG Date for control with rare codon number
    3. [http://partsregistry.org/wiki/index.php?title=Part:BBa_K567021 Main Page] -PT7-Luc-2x4AGG(111bp) Date for control with rare codon number, locations and tandem copies



    Stop-Codon Switch

    11SJTU stop 01.jpg
    • Data for our favorite new parts
    1. [http://partsregistry.org/wiki/index.php?title=Part:BBa_K567011 Main Page] - BBa_K567011 PT7-TDRS :Aspartyl aminoacyl tRNA synthetase without anticodon recognition domain under the control of T7 promoter and lac operator.
    2. [http://partsregistry.org/wiki/index.php?title=Part:BBa_K567013 Main Page] - BBa_K567013 tRNAAsp-TAG : The tRNAAsp-TAG with its anticodon mutated to CUA (base pairing stop codon UAG) and under the control of aspV promoter.
    • We've Also Characterized the Following Part
    1. [http://partsregistry.org/wiki/index.php?title=Part:BBa_K567003 Main Page] - BBa_K567003 Pbla-Luc-TAG
    • We've Also Made the Following Part
    1. [http://partsregistry.org/wiki/index.php?title=Part:BBa_K567018 Main Page] - BBa_K567018 PT7-GFP-TAG-RFP



    Initial-Codon Switch

    • Data for our favorite new parts
    1. [http://partsregistry.org/wiki/index.php?title=Part:BBa_K567014 Main Page] - BBa_K567014 PT7-metGM : T7 promoter-metG(mutated). This biobrick is constructed by putting the mutated metG (Met-RS) under the control of T7 promoter and lac operator.
    2. [http://partsregistry.org/wiki/index.php?title=Part:BBa_K567015 Main Page] - BBa_K567015 PT7-metGN :T7 promoter-metG(truncated). This biobrick is constructed by putting the truncated metG (Met-RS) under the control of T7 promoter and lac operator.
    3. [http://partsregistry.org/wiki/index.php?title=Part:BBa_K567016 Main Page] - BBa_K567016 metY-CGA :This biobrick is constructed by mutating the anticodon of tRNAmet to TCG (base pairing codon CGA).
    • We've Also Characterized the Following Part
    1. [http://partsregistry.org/wiki/index.php?title=Part:BBa_K567020 Main Page] - BBa_K567020 KanR-CGA


    Positive FeedBack

    • Data for our new parts
    1. [http://partsregistry.org/wiki/index.php?title=Part:BBa_K567022 Main Page] - PT7-Luc-nAGG-tRNA(Arg). This biobrick is under the control of T7 promoter and lac operator.
    Retrieved from "http://2011.igem.org/Team:SJTU-BioX-Shanghai/Parts/Data_Page"