Team:Osaka/week12

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Latest revision as of 13:27, 23 October 2011

October 16 (Sun)

Reginal Jamboree in HKUST

October 17 (Mon)

October 18 (Tue)

Restriction digests
Ligation
- 116(T):105(upstream)+1-23L(downstream)+1-7A(vector)
- 117(T):106(upstream)+1-23L(downstream)+1-7A(vector)
- 118(T):107(upstream)+1-23L(downstream)+1-7A(vector)
- 119(T):108(upstream)+1-23L(downstream)+1-7A(vector)
Transformation of Ligation products

October 19 (Wed)

To liquid culture:116,117,118,119
Plating
Ligation
- 120(T):105(upstream)+1-23L(downstream)+1-5A(vector)
- 121(T):106(upstream)+1-23L(downstream)+1-5A(vector)
- 122(T):107(upstream)+1-23L(downstream)+1-5A(vector)
- 123(T):108(upstream)+1-23L(downstream)+1-5A(vector)
Transformation of ligation products

October 20 (Thu)

Miniprep of yesterday's culture
Restriction digests of miniprepped parts
Electrophoresis of digests
- 118 x
Ligation
- 124(C):116(upstream)+117(downstream)+1-3A(vector)
- 125(C):116(upstream)+119(downstream)+1-3A(vector)
- 126(C):117(upstream)+119(downstream)+1-3A(vector)
To liquid culture:118
Transformation of ligation products

October 21 (Fri)

Miniprep of yesterday's culture
Restriction digests of miniprepped parts
Electrophoresis of digests
Ligation
- 127(C):116(upstream)+118(downstream)+1-3A(vector)
- 128(C):117(upstream)+118(downstream)+1-3A(vector)
- 129(C):118(upstream)+119(downstream)+1-3A(vector)
To liquid culture:124,125,126
Transformation of ligation products
Plating (Tolerance assay)

October 22 (Sat)

Colony counts (Tolerance assay)
Miniprep of yesterday's culture
Restriction digests of miniprepped parts
Electrophoresis of digests
Gel extraction
Ligation
- 130(C):124(upstream)+118(downstream)+1-3A(vector)
- 131(C):124(upstream)+119(downstream)+1-3A(vector)
- 132(C):126(upstream)+118(downstream)+1-3A(vector)
- 133(C):01+1-3A(vector)
- 134(C):02+1-3A(vector)
- 135(C):1-12M+1-3A(vector)
- 136(C):01(upstream)+119(downstream)+1-3A(vector)
- 137(C):02(upstream)+119(downstream)+1-3A(vector)
- 138(C):116+1-3A(vector)
- 139(C):117+1-3A(vector)
- 140(C):118+1-3A(vector)
- 141(C):119+1-3A(vector)
Transformation of products

Back to Notebook

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@@ Line 28: / Line 28: @@
 ==October 20 (Thu)==
+# Miniprep of yesterday's culture
+# Restriction digests of miniprepped parts
+# Electrophoresis of digests
+#* 118 x
+# Ligation
+#* 124(C):116(upstream)+117(downstream)+1-3A(vector)
+#* 125(C):116(upstream)+119(downstream)+1-3A(vector)
+#* 126(C):117(upstream)+119(downstream)+1-3A(vector)
+# To liquid culture:118
+# Transformation of ligation products
 ==October 21 (Fri)==
+# Miniprep of yesterday's culture
+# Restriction digests of miniprepped parts
+# Electrophoresis of digests
+# Ligation
+#* 127(C):116(upstream)+118(downstream)+1-3A(vector)
+#* 128(C):117(upstream)+118(downstream)+1-3A(vector)
+#* 129(C):118(upstream)+119(downstream)+1-3A(vector)
+# To liquid culture:124,125,126
+# Transformation of ligation products
+# Plating (Tolerance assay)
+==October 22 (Sat)==
+# Colony counts (Tolerance assay)
+# Miniprep of yesterday's culture
+# Restriction digests of miniprepped parts
+# Electrophoresis of digests
+# Gel extraction
+# Ligation
+#* 130(C):124(upstream)+118(downstream)+1-3A(vector)
+#* 131(C):124(upstream)+119(downstream)+1-3A(vector)
+#* 132(C):126(upstream)+118(downstream)+1-3A(vector)
+#* 133(C):01+1-3A(vector)
+#* 134(C):02+1-3A(vector)
+#* 135(C):1-12M+1-3A(vector)
+#* 136(C):01(upstream)+119(downstream)+1-3A(vector)
+#* 137(C):02(upstream)+119(downstream)+1-3A(vector)
+#* 138(C):116+1-3A(vector)
+#* 139(C):117+1-3A(vector)
+#* 140(C):118+1-3A(vector)
+#* 141(C):119+1-3A(vector)
+# Transformation of products
-==October 22 (Sat)==
 [[Team:Osaka/Notebook|Back to Notebook]]
 week [[Team:Osaka/week1|1]]|[[Team:Osaka/week2|2]]|[[Team:Osaka/week3|3]]|[[Team:Osaka/week4|4]]|[[Team:Osaka/week5|5]]|[[Team:Osaka/week6|6]]|[[Team:Osaka/week7|7]]|[[Team:Osaka/week8|8]]|[[Team:Osaka/week9|9]]|[[Team:Osaka/week10|10]]|[[Team:Osaka/week11|11]]|'''12'''|[[Team:Osaka/week13|13]]