Team:SJTU-BioX-Shanghai

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==BioBricks==
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__TOC__
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===Modulator===
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'''Modulators''' control the amount of charged tRNAs that recognize rare/stop codons. These include tRNA Modulators, aaRS Modulators, Stop Codon Modulators and Initial Codon Modulators.
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</head>
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====tRNA Modulators:====
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<body>
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<div id="cnt"><h2>Countdown</h2><embed src="http://www.oneplusyou.com/bb/files/countdown/countdown.swf?co=0066ff&bgcolor=eeeeee&date_month=10&date_day=15&date_year=2011&un=iGEM World Jamboree 2011&size=normal&mo=11&da=5&yr=2011" type="application/x-shockwave-flash" pluginspage="http://www.macromedia.com/go/getflashplayer" width="200 px" height="60 px" wmode="transparent"></embed></div>
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====='''BBa_K567001  ''LacI'' -Ptrc-tRNA<sup>Arg</sup>''' (Favorite Part)=====
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<div id="vst"><h2>Visitors</h2><a href="http://www2.clustrmaps.com/user/8c3de420"><img src="http://www2.clustrmaps.com/stats/maps-no_clusters/2011.igem.org-Team-SJTU-BioX-Shanghai-thumb.jpg" alt="Locations of visitors to this page" /></a></div>
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Description: ''LacI''- Ptrc derived from plasmid pTrc99B and tRNA<sup>Arg</sup> from ''E.coli'' ''ArgW'' operon. The tRNA<sup>Arg</sup> is under the control of promoter trc. tRNA<sup>Arg</sup> expression is induced by 0.5mM IPTG when the OD600 of the culture reaches 0.3. This part is constructed on the backbone plasmid pACYC184.
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<div id="sps"><h2>Sponsors</h2>
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[[image:11SJTU-TRC.jpg|LacI -Ptrc-tRNA<sup>Arg</sup>]]
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<div class="s">
 +
<a href="http://www.merckmillipore.com/millipore"><img src="/wiki/images/e/ec/11SJTU_merck.jpg" alt="Merck millipore" height="80 px" /></a><h5>Merck Millipore</h5></div>
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====='''BBa_K567002  ''sulA'' promoter-tRNA<sup>Arg</sup>'''=====
 
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Description: ''sulA'' promoter derived from ''E.coli'' ''sulA'' operon and tRNA<sup>Arg</sup> from ''E.coli'' ''ArgW'' operon. The tRNA<sup>Arg</sup> is under the control of ''sulA'' promoter induced by UV. tRNA expression is induced by 20-second UV exposure (the distance between the 20W lamp and the culture is 35cm) when the OD600 of the culture reaches 0.3. This part is constructed on the backbone plasmid pACYC184.
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<div class="s">
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<a href="http://www.sjtu.edu.cn"><img src="/wiki/images/7/70/11SJTU-SJTU-logo.jpg" alt="Shanghai Jiao Tong University" height="50 px" margin="auto" /></a><h5>Shanghai Jiao Tong University</h5></div>
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[[image:11SJTU-UV.jpg|''sulA'' promoter-tRNA(Arg)]]
 
 +
<div class="s">
 +
<a href="http://www.bio-x.cn"><img src="/wiki/images/8/81/11SJTU_bio-x.jpg" alt="Bio-X Institutes" height="50px"/></a><h5>Bio-X Institutes</h5></div>
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====aaRS Modulators: ====
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<div class="s">
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<a href="http://www.genscript.com/"><img src="/wiki/images/7/7c/11SJTU-Genscript.jpg" alt="GenScript" height="50px"/></a><h5>GenScript</h5></div>
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====='''BBa_K567012  tRNA<sup>Asp</sup>-AGG '''(Favorite Part)=====
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</div>
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Description: tRNA<sup>Asp</sup> with its anticodon mutated to CCU(base pairing rare codon AGG) and under the control of ''lpp'' promoter. This biobrick is constructed first by cloning the tRNA<sup>Asp</sup> from ''AspV'' in ''E.coli'', then the anticodon region is site-directed mutated. This part is constructed on the backbone plasmid pACYC184.
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</body>
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[[image:11SJTU-tRNA-asp-AGG.jpg|tRNA<sup>Asp</sup>-AGG]]
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{{Template:11SJTU_Nav}}
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<html>
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====='''BBa_K567011  PT7-TDRS''' (Favorite Part)=====
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<head>
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<style>  
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Description: aspartyl aminoacyl tRNA synthetase without anticodon recognition domain under the control of T7 promoter and ''lac'' operator. This biobrick is constructed by deleting the anticodon recognition domain of AspRS from ''E.coli''. This modified AspRS can charge Asp to tRNA<sup>Asp</sup>-TAG (BBa_K567013) and tRNA<sup>Asp</sup>-AGG (BBa_K567012). This part is constructed by inserting the truncated fragment of Gene ''AspS'' into the multiple cloning site on the plasmid pET28a.
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====Stop-Codon Modulators: ====
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div#container{ float:left;width:976px; background:#fff; }
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div#side{float:left; width:240px; background:#eee;height:1750px;border-right: 2px solid #fff; }
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====='''BBa_K567013  tRNA<sup>Asp</sup>-TAG ''' (Favorite Part)=====
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div#swf{float:right; width:660px;height:400px; margin:0 40px 0 0; }
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Description: tRNA<sup>Asp</sup> with its anticodon mutated to CUA (base pairing stop codon UAG) and under the control of ''lpp'' promoter. This biobrick is constructed first by cloning the tRNA<sup>Asp</sup> from ''AspV'' in ''E.coli'', then the anticodon region is site-directed mutated. This part is constructed on the backbone plasmid pACYC184.
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div#dtl{float:right; width:660px;margin:10px 40px 0 0; }
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.title{ font-size:1.6em;padding:10px ;margin:0;color:#d80;}
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[[image:11SJTU-tRNA-asp-TAG.jpg|tRNA<sup>Asp</sup>-TAG]]
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div#pro{float:left;padding:0 10px 10px 10px;height:380px;margin:0;}
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div .box{float:left; margin:10px 6px; width:200px; height:200px; border:3px solid #ddd; }
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====='''BBa_K567011  PT7-TDRS'''=====
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div#achieve{float:left;padding:0 10px 10px 10px;border-top:1px solid #ddd;border-bottom:3px solid #e1b03a;margin:0;}
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</style>  
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Description: aspartyl aminoacyl tRNA synthetase without anticodon recognition domain under the control of T7 promoter and ''lac'' operator. This biobrick is constructed by deleting the anticodon recognition domain of AspRS from ''E.coli''. This modified AspRS can charge Asp to tRNA<sup>Asp</sup>-TAG (BBa_K567013) and tRNA<sup>Asp</sup>-AGG (BBa_K567012). This part is constructed by inserting the truncated fragment of Gene ''AspS'' into the multiple cloning site on the plasmid pET28a.
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</head>
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<body>
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====Initial-Codon Modulators:====
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<div id=container>
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<div id="side">
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====='''BBa_K567014 PT7-''metG''M'''=====
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</div>
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Description: T7 promoter-''metG''(mutated). This biobrick is constructed by putting the mutated metG (Met-RS) under the control of T7 promoter and ''lac'' operator. We have cloned ''metG'' from ''E.coli'' and have used error-prone PCR to amplify the ''metG''. Kana gene with start codon substituted for CGA is used to testify the function of mutated metG. When this biobrick and ''metY''-CGA (BBa_K567016) are co-transformed into the cell, the cells can survive on the LBKana plate. This part is constructed on by inserting the Gene ''metG'' fragment with random mutation into the multiple cloning site on the plasmid pET28a.
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<div class="title"><b>The Project: Codon Switch Controlling Protein Biosynthesis</b></div>
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<div id=swf>
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====='''BBa_K567015  PT7-''metG''N'''=====
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<embed src="/wiki/images/7/71/11SJTU_home.swf" wmode="transparent" height="380 px" width="660 px" > </embed>
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Description: T7 promoter-''metG''(truncated). This biobrick is constructed by putting the truncated metG (Met-RS) under the control of T7 promoter and ''lac'' operator. We have cloned ''metG'' from ''E.coli'' and the tRNA recognition domain of metG is truncated. Kana gene with start codon substituted for CGA is used to testify the function of mutated metG. When this biobrick and ''metY''-CGA (BBa_K567016) are co-transformed into the cell, the cells can survive on the LBKana plate. This part is constructed on by inserting the truncated Gene ''metG'' fragment into the multiple cloning site on the plasmid pET28a.
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</div>
-
 
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====='''BBa_K567016  ''metY''-CGA'''=====
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-
Description: This biobrick is constructed by mutating the anticodon of tRNAmet to TCG (base pairing codon CGA). This tRNA can transfer fMet to CGA when it is used as the start codon. Kana gene with start codon substituted for CGA is used to testify the function of ''metY''-CGA. When this biobrick and ''metG''M(BBa_K567014) or ''metG''N(BBa_K567015) are co-transformed into the cell, the cells can survive on the LBKana plate. This part is constructed on the backbone plasmid pACYC184.
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-
 
+
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[[image:11SJTU-tRNA-met-CGA.jpg|metY-CGA]]
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-
 
+
-
 
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-
----
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-
 
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-
===Reporter===
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-
 
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-
'''Reporters''': we use two sets of Reporters to test the function of our Modulators.
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-
 
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====Reporter for Quantitative analysis:====
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-
 
+
-
====='''BBa_K567003  P''bla''-Luc-TAG''' =====
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-
 
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-
Description: Luciferase under the control of β-lactamase promoter with TAG insertion. A TAG codon is inserted after the initial codon of the gene. This part is used to testify the function of corporation of PT7-TDRS (BBa_K567011) and tRNA<sup>Asp</sup>-TAG (BBa_K567013). β-lactamase promoter is derived from pUC18 β-lactamase operon. Wild type luciferase from BBa_I712019. This part is constructed on the plasmid pET28a as backbone.
+
-
 
+
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[[image:11SJTU-LUC-TAG.jpg|Pbla-Luc-TAG]]
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-
 
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-
====='''BBa_K567004    P''bla''-Luc-2AGG'''=====
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-
 
+
-
Description: β-lactamase promoter-luciferase with two AGG-codon insertions. This biobrick is constructed by putting modified enzyme luciferase under constituitive promoter β-lactamase promoter. 2 AGG codons and 2 GCG codons are inserted after the ATG start codon of wild type luciferase (BBa_I712019). Modified luciferase keeps the activity of converting luciferin into oxyluciferin, during which bioluminescence will emit. This part is one of the reporter genes to testify the influence of different number of rare codons in regulating protein biosynthesis. This part is used as a measurement to testify the function of ''LacI'' -Ptrc-tRNA<sup>Arg</sup>(BBa_K567001) or ''sulA'' promoter-tRNA<sup>Arg</sup> (BBa_K567002).
+
-
 
+
-
Cell is cultured in 50ug/ml kanamycin and 10ug/ml tetracycline LB liquid medium. When the OD600 of the culture reaches 0.3 IPTG is added to make the final concentration 0.5nM to induce the synthesis of tRNA.
+
-
 
+
-
Ultrasonication is used to release the luciferase from the cell. Sonics ON 3 seconds, OFF 3 seconds, total ultrasonication time 3 minutes.
+
-
 
+
-
Amount of bioluminescence produced can be detected using luminometer.
+
-
 
+
-
β-lactamase promoter is derived from pUC18 β-lactamase operon. Wild type luciferase from BBa_I712019. This part is constructed on the plasmid pET28a as backbone.
+
-
 
+
-
Point mutation is used to obtain this part from wild type. '''BBa_K567005  P''bla''-Luc-4AGG, BBa_K567006  P''bla''-Luc-6AGG and BBa_K567007  P''bla''-Luc-8AGG''' are constructed in the same way.
+
-
 
+
-
====='''BBa_K567005    P''bla''-Luc-4AGG'''=====
+
-
 
+
-
Description: β-lactamase promoter-Luciferase with four AGG-codon insertions. Point mutation is used to obtain this part from wild type. BBa_K567005  P''bla''-Luc-4AGG is constructed in the same way as '''BBa_K567004    P''bla''-Luc-2AGG'''.
+
-
 
+
-
====='''BBa_K567006    P''bla''-Luc-6AGG'''=====
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-
 
+
-
Description: β-lactamase promoter-Luciferase with six AGG-codon insertions. Point mutation is used to obtain this part from wild type. BBa_K567006  P''bla''-Luc-6AGG is constructed in the same way as '''BBa_K567004    P''bla''-Luc-2AGG'''.
+
-
 
+
-
====='''BBa_K567007    P''bla''-Luc-8AGG'''=====
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-
 
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-
Description: β-lactamase promoter-Luciferase with eight AGG-codon insertions. Point mutation is used to obtain this part from wild type. BBa_K567007  P''bla''-Luc-8AGG is constructed in the same way as '''BBa_K567004    P''bla''-Luc-2AGG'''.
+
-
 
+
-
[[image:11SJTU-bla-AGG-luc.jpg|600px|Pbla-Luc]]
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-
 
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====='''BBa_K567008  PT7-Luc-2AGG'''=====
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-
Description: this biobrick is constructed by putting modified enzyme luciferase under promoter T7 and controlled by lac operator. 2 AGG codons and 2 GCG codons are inserted after the ATG start codon of wild type luciferase (BBa_I712019). Modified luciferase keeps the activity of converting luciferin into oxyluciferin, during which bioluminescence will emit. This part is one of the reporter genes to testify the influence of different number of rare codons in regulating protein biosynthesis. This part is used as a measurement to testify the function of ''LacI'' -Ptrc-tRNA<sup>Arg</sup> (BBa_K567001) or ''sulA'' promoter-tRNA<sup>Arg</sup> (BBa_K567002).
+
-
 
+
-
Cell is cultured in 50ug/ml kanamycin 10ug/ml tetracycline LB liquid medium. When the OD600 of the culture reaches 0.3 IPTG is added to make the final concentration 0.5nM to induce the synthesis of tRNA.
+
-
 
+
-
Ultrasonication is used to release the luciferase from the cell. Sonics ON 3 seconds, OFF 3 seconds, total ultrasonication time 3minutes.
+
-
 
+
-
Amount of bioluminescence produced can be detected using luminometer.
+
-
T7 promoter is derived from pET28a. Wild type Luciferase derived from BBa_I712019.  
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<div id="dtl">
 +
<div id="pro">
 +
<p>SJTU-BioX-Shanghai iGEM team is designing a set of Codon-Switches that regulate target protein biosynthesis (translation).</p>
 +
<p>In our <a href="/Team:SJTU-BioX-Shanghai/Project/Subproject1"><b>Rare-Codon Switch</b></a>, the translation of the protein can be finely turned up/down with the control of  <a href="/Team:SJTU-BioX-Shanghai/Project/Subproject1-1">rare tRNA amount</a>,  <a href="/Team:SJTU-BioX-Shanghai/Project/Subproject1-2">aaRS that charges the rare tRNA</a> and <a href="/Team:SJTU-BioX-Shanghai/Project/Subproject1-3">rare codons</a>.  
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'''BBa_K567009  PT7-Luc-4AGG, BBa_K567010  PT7-Luc-8AGG and BBa_K567019  PT7-Luc-6AGG''' are constructed in the same way.
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<p>Besides, our device can be made into switches that can be turned on/off without background noise in two ways. One is to use stop codon as the controlling element, the <a href="/Team:SJTU-BioX-Shanghai/Project/Subproject2"><b>Stop-Codon Switch</b></a>. The other is to use any codon but the original start codon to initiate translation, the <a href="/Team:SJTU-BioX-Shanghai/Project/Subproject3"><b>Initial-Codon Switch</b></a>.</p>
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====='''BBa_K567009  PT7-Luc-4AGG'''=====
 
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Description: this biobrick is constructed by putting modified enzyme luciferase under promoter T7 and controlled by lac operator.This biobrick is constructed in the same way as '''BBa_K567008  PT7-Luc-2AGG'''.
 
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====='''BBa_K567019  PT7-Luc-6AGG'''=====
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<img src="/wiki/images/e/e2/11SJTU_home_fig1.jpg"  alt="Project" style="float:right;"/>
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Description: this biobrick is constructed by putting modified enzyme luciferase under promoter T7 and controlled by lac operator.This biobrick is constructed in the same way as '''BBa_K567008  PT7-Luc-2AGG'''.
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====='''BBa_K567010  PT7-Luc-8AGG'''=====
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<p>Our design has expanded the regulating tools for synthetic biology and introduced brand-new methods for protein function analysis. See more about project applications, click <a href="/Team:SJTU-BioX-Shanghai/Project/Application"><b>here</b></a>.</p>
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Description: this biobrick is constructed by putting modified enzyme luciferase under promoter T7 and controlled by lac operator.This biobrick is constructed in the same way as '''BBa_K567008  PT7-Luc-2AGG'''.  
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</div>
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[[image:11SJTU-T7-AGG-luc.jpg|600px|PT7-Luc]]
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<div class="box"><a href="/Team:SJTU-BioX-Shanghai/Team"><img src="/wiki/images/d/d2/11SJTU_home_tm.jpg"  alt="Team"/></a></div>
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<div class="box"><a href="/Team:SJTU-BioX-Shanghai/Parts/Data_Page"><img src="/wiki/images/c/c7/11SJTU_home_pt.jpg"  alt="Parts"/></a></div>
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<div class="box"><a href="/Team:SJTU-BioX-Shanghai/Human_practice"><img src="/wiki/images/c/c6/11SJTU_home_hp.jpg"  alt="Human practice"/></a></div>
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</div>
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<div id="dtl">
 +
<div class="title"><b>Achievements</b></div>
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<div id="achieve">
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<img src="/wiki/images/3/3a/11SJTU_cheering.jpg" style="float:right;margin:10px;">
 +
<p><img src="/wiki/images/7/7f/11SJTU_r.png"  alt="right" />
 +
<b>Gold Medal</b> and <b>Best New BioBrick Part or Device, Engineered</b>: <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K567011">BBa_K567011</a> & <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K567012">BBa_K567012</a> at Asia Jamboree. Advancing to World Champion!</p>
 +
<p><img src="/wiki/images/7/7f/11SJTU_r.png"  alt="right" />
 +
Constructed three Codon Switches that controls protein biosynthesis, <a href="/Team:SJTU-BioX-Shanghai/Project/Subproject1">Rare-Codon Switch</a>, <a href="/Team:SJTU-BioX-Shanghai/Project/Subproject2">Stop-Codon Switch</a> and <a href="/Team:SJTU-BioX-Shanghai/Project/Subproject3">Initial-Codon Switch</a>. </p>
 +
<p><img src="/wiki/images/7/7f/11SJTU_r.png"  alt="right" />
 +
Used <a href="/Team:SJTU-BioX-Shanghai/Project/Subproject1">Rare-Codon Switch</a> to turn up/down protein biosynthesis by controlling <a href="/Team:SJTU-BioX-Shanghai/Project/Subproject1-1">rare tRNA amount</a>, <a href="/Team:SJTU-BioX-Shanghai/Project/Subproject1-2">aaRS</a> and <a href="/Team:SJTU-BioX-Shanghai/Project/Subproject1-3">the number of rare codons.</a> </p>
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====Reporter for Qualitative Analysis:====
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<p><img src="/wiki/images/7/7f/11SJTU_r.png"  alt="right" />
 +
Built our Rare-Codon Switch <a href="/Team:SJTU-BioX-Shanghai/Project/Subproject1/Modeling"><i>in silic</i></a> and demonstrated the reliability of the model through our experiment results.
 +
</p>
-
====='''BBa_K567017 PT7-RFP-6AGG'''=====
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<p><img src="/wiki/images/7/7f/11SJTU_r.png"  alt="right" />
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Description: RFP with 6 AGG-codon insertions after ATG under the control of T7 promoter and lac operator. The protein can be expressed successfully when co-transformed with PT7-TDRS (BBa_K567011) and tRNA<sup>Asp</sup>-AGG (BBa_K567012). Modified RFP exhibits lower red fluorescence brightness. This part is used to testify the function of PT7-TDRS (BBa_K567011). This part is constructed by inserting the RFP-6AGG fragment into the multiple cloning site on the plasmid pET28a.
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Constructed two strict switches, <a href="/Team:SJTU-BioX-Shanghai/Project/Subproject2">Stop-Codon Switch</a> and <a href="/Team:SJTU-BioX-Shanghai/Project/Subproject3">Initial-Codon Switch</a>, that can turn on/off protein biosynthesis. </p>
 +
<p><img src="/wiki/images/7/7f/11SJTU_r.png"  alt="right" />
 +
Demonstrated <b>device application</b> by constructing and successfully testing <a href="/Team:SJTU-BioX-Shanghai/Project/Application">positive feedback</a> with our device in metabolic pathway. </p>
-
[[image:11SJTU-RFP-6AGG.jpg|PT7-RFP-6AGG]]
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<p><img src="/wiki/images/7/7f/11SJTU_r.png"  alt="right" />
 +
Constructed <a href="/Team:SJTU-BioX-Shanghai/Parts">27 biobricks</a> in our 3 sub-projects.  
 +
</p>
 +
<p><img src="/wiki/images/7/7f/11SJTU_r.png"  alt="right" />
 +
Analysed the structures of 18 aaRS with <a href="/Team:SJTU-BioX-Shanghai/Project/Application-3">structural biological methods</a>. Designed structures of 14 modified aaRS deprived of their anticodon recognition ability.
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</p>
 +
<p><img src="/wiki/images/7/7f/11SJTU_r.png"  alt="right" />
 +
Expanded the <a href="/Team:SJTU-BioX-Shanghai/Project/Application">regulating tools</a> for synthetic biology and developed two brand-new ways that facilitate the study of protein function.
 +
</p>
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====='''BBa_K567018 PT7-GFP-TAG-RFP'''=====
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<p><img src="/wiki/images/7/7f/11SJTU_r.png"  alt="right" />
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Description: GFP and RFP linked with a flexible chain and a stop codon TAG is inserted in the flexible chain. This biobrick is under the control of T7 promoter and lac operator. This part is used to testify the function of PT7-TDRS (BBa_K567011) and tRNA<sup>Asp</sup>-TAG (BBa_K567013). This part is constructed by inserting the GFP-TAG-RFP fragment into the multiple cloning site on the plasmid pET28a.
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Designed our Human Practice Project: <a href="/Team:SJTU-BioX-Shanghai/Human_practice">T-PostCards</a> to promote our views on synthetic biology through communications and connections.
 +
</p>
 +
<p><img src="/wiki/images/7/7f/11SJTU_r.png"  alt="right" />
 +
Promoted synthetic biology <a href="/Team:SJTU-BioX-Shanghai/Human_practice_2">in high school class</a>. Designed a questionnaire and did a survey on high school students' understandings and attitudes towards synthetic biology.  
 +
</p>
 +
<p><img src="/wiki/images/7/7f/11SJTU_r.png"  alt="right" />
 +
Attended the <b>2011 China Meet Up</b> and presented our project idea to other iGEMer and advisors. </p>
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[[image:11SJTU-GFP-TAG-RFP.jpg|PT7-GFP-TAG-RFP]]
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</div>
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</div>
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</body>
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</html>

Latest revision as of 03:52, 29 October 2011

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    The Project: Codon Switch Controlling Protein Biosynthesis

    SJTU-BioX-Shanghai iGEM team is designing a set of Codon-Switches that regulate target protein biosynthesis (translation).

    In our Rare-Codon Switch, the translation of the protein can be finely turned up/down with the control of rare tRNA amount, aaRS that charges the rare tRNA and rare codons.

    Besides, our device can be made into switches that can be turned on/off without background noise in two ways. One is to use stop codon as the controlling element, the Stop-Codon Switch. The other is to use any codon but the original start codon to initiate translation, the Initial-Codon Switch.

    Project

    Our design has expanded the regulating tools for synthetic biology and introduced brand-new methods for protein function analysis. See more about project applications, click here.

    Team
    Parts
    Human practice
    Achievements

    right Gold Medal and Best New BioBrick Part or Device, Engineered: BBa_K567011 & BBa_K567012 at Asia Jamboree. Advancing to World Champion!

    right Constructed three Codon Switches that controls protein biosynthesis, Rare-Codon Switch, Stop-Codon Switch and Initial-Codon Switch.

    right Used Rare-Codon Switch to turn up/down protein biosynthesis by controlling rare tRNA amount, aaRS and the number of rare codons.

    right Built our Rare-Codon Switch in silic and demonstrated the reliability of the model through our experiment results.

    right Constructed two strict switches, Stop-Codon Switch and Initial-Codon Switch, that can turn on/off protein biosynthesis.

    right Demonstrated device application by constructing and successfully testing positive feedback with our device in metabolic pathway.

    right Constructed 27 biobricks in our 3 sub-projects.

    right Analysed the structures of 18 aaRS with structural biological methods. Designed structures of 14 modified aaRS deprived of their anticodon recognition ability.

    right Expanded the regulating tools for synthetic biology and developed two brand-new ways that facilitate the study of protein function.

    right Designed our Human Practice Project: T-PostCards to promote our views on synthetic biology through communications and connections.

    right Promoted synthetic biology in high school class. Designed a questionnaire and did a survey on high school students' understandings and attitudes towards synthetic biology.

    right Attended the 2011 China Meet Up and presented our project idea to other iGEMer and advisors.

    Retrieved from "http://2011.igem.org/Team:SJTU-BioX-Shanghai"