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Team:Panama/Protocols/Wetlab
From 2011.igem.org
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1. [[Agarose gel electrophoresis]] | 1. [[Agarose gel electrophoresis]] | ||
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7. [[Ampicillin stock]] | 7. [[Ampicillin stock]] | ||
| - | 8.[[ | + | 8.[[Chloramphenicol stock]] |
9. [[Mutagenesis PCR]] | 9. [[Mutagenesis PCR]] | ||
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17. [[Primer aliquots]] | 17. [[Primer aliquots]] | ||
| - | 18. [[Cell Culture]] | + | 18. [[ "Overnight" Cell Culture]] |
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| + | 19. [[NZY+ Recipe]] | ||
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| + | 20. [[Gel DNA extraction]] | ||
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| + | 21. [[SOC Recipe]] | ||
[[File:home.jpg|100px|link=https://2011.igem.org/Team:Panama]] | [[File:home.jpg|100px|link=https://2011.igem.org/Team:Panama]] | ||
Latest revision as of 01:46, 29 September 2011
Wetlab
1. Agarose gel electrophoresis3. Colony PCR
4. Pgem Cloning
5. Preparing chemically competent cells
10. Mutagenesis transformation
12. Miniprep "Dirty"
13. PCR purification
14. Glycerol Stock
15. PCR
16. Agar plates
17. Primer aliquots
19. NZY+ Recipe
21. SOC Recipe
