Team:Osaka/week7

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Latest revision as of 05:51, 19 October 2011

September 11 (Sun)

Gel extraction of digests
Miniprep of yesterday's culture
Restriction digests of minipreppped parts
Gel electrophoresis of digests
Ligation
- 103(C):PprM+1-3A(vector)
- 104(C):RecA+1-3A(vector)
- 105(A):12(upstream)+101(downstream)+1-1G(vector)
- 106(A):12(upstream)+102(downstream)+1-1G(vector)
Transformation of ligation products

September 12 (Mon)

To liquid culture:103,104,105,106

September 13 (Tue)

Miniprep of yesterday's culture
Restriction digests of minipreppped parts
Gel electrophoresis of digests
Ligation
- 107(A):12(upstream)+103(downstream)+1-1G(vector)
- 108(A):12(upstream)+104(downstream)+1-1G(vector)
Transformation of ligation products

September 14 (Wed)

To liquid culture:107,108

September 15 (Thu)

Miniprep of yesterday's culture
Restriction digests of minipreppped parts
Gel electrophoresis of digests
To liquid culture:108

September 16 (Fri)

Miniprep of yesterday's culture
Restriction digests of minipreppped parts
Gel electrophoresis of digests

September 17 (Sat)

Ligation
- 109(K):1-2M(upstream)+101(downstream)+1-5A(vector)
- 110(K):1-2M(upstream)+102(downstream)+1-5A(vector)
- 111(K):1-2M(upstream)+103(downstream)+1-5A(vector)
- 112(K):1-2M(upstream)+104(downstream)+1-5A(vector)
Transformation of ligation products

Back to Notebook

week 1|2|3|4|5|6|7|8|9|10|11|12|13

@@ Line 1: / Line 1: @@
 {{Osaka}}
+__NOTOC__
+==September 11 (Sun)==
-==September 11(Sun)==
 # Gel extraction of digests
 # Miniprep of yesterday's culture
@@ Line 15: / Line 14: @@
-==September 12(Mon)==
+==September 12 (Mon)==
 # To liquid culture:103,104,105,106
-==September 13(Tue)==
+==September 13 (Tue)==
 # Miniprep of yesterday's culture
 # Restriction digests of minipreppped parts
@@ Line 27: / Line 26: @@
 # Transformation of ligation products
-==September 14(Wed)==
+==September 14 (Wed)==
 # To liquid culture:107,108
-==September 15(Thu)==
+==September 15 (Thu)==
+# Miniprep of yesterday's culture
+# Restriction digests of minipreppped parts
+# Gel electrophoresis of digests
+# To liquid culture:108
+==September 16 (Fri)==
+# Miniprep of yesterday's culture
+# Restriction digests of minipreppped parts
+# Gel electrophoresis of digests
-==September 16(Fri)==
+==September 17 (Sat)==
-==September 17(Sat)==
+# Ligation
+#* 109(K):1-2M(upstream)+101(downstream)+1-5A(vector)
+#* 110(K):1-2M(upstream)+102(downstream)+1-5A(vector)
+#* 111(K):1-2M(upstream)+103(downstream)+1-5A(vector)
+#* 112(K):1-2M(upstream)+104(downstream)+1-5A(vector)
+# Transformation of ligation products
 [[Team:Osaka/Notebook|Back to Notebook]]
+week [[Team:Osaka/week1|1]]|[[Team:Osaka/week2|2]]|[[Team:Osaka/week3|3]]|[[Team:Osaka/week4|4]]|[[Team:Osaka/week5|5]]|[[Team:Osaka/week6|6]]|'''7'''|[[Team:Osaka/week8|8]]|[[Team:Osaka/week9|9]]|[[Team:Osaka/week10|10]]|[[Team:Osaka/week11|11]]|[[Team:Osaka/week12|12]]|[[Team:Osaka/week13|13]]