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Team:British Columbia/Objectives
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==Wet Lab== | ==Wet Lab== | ||
| - | + | '''Production of Monoterpenes in Yeast''' | |
| - | From an environmental | + | From an industrial perspective, this yeast will be cultured and harvested for monoterpenes, which will be analysed by gas chromatography mass spectrometry (GC-MS). Yeast is a highly utilized organism in industry (e.g. breweries and also the recently established Amyris biotech company that uses yeast to produce anti-Malarial therapeutics amongst other valuable compounds). We envision that monoterpene-producing yeast will not only be industrially profitable, but also help to alleviate the unsustainable consumption of nature's resources in the quest for biofuels, pharmaceuticals, flavours/fragrances etc. |
| + | |||
| + | From an environmental perspective, this yeast will be co-cultured with the bluestain fungus to determine if monoterpene-producing yeast is able to inhibit the growth of the fungus. This yeast is also a biological system that can be screened for potential targets against the fungus. For instance, since the yeast is actively producing monoterpenes, it is possible to look for yeast that have particular mutations that cause increased intolerance against monoterpenes. These mutations will reveal potential strategies against the fungus. | ||
'''Monoterpene Synthases''' | '''Monoterpene Synthases''' | ||
| - | + | Our goal is to express synthases that produce monoterpenes (specifically limonene, pinene, carene and cineole) in yeast. Genes encoding these synthases will be inserted into yeast gateway vectors as well as the biobrick plasmid PSB1C3. | |
'''Metabolic Genes''' | '''Metabolic Genes''' | ||
| + | |||
| + | Our goal is to express metabolic enzymes (specifically, erg20-2, Idi1 and Hmg2) that optimize the mevalonate pathway in yeast for monoterpene production. | ||
| + | |||
| + | <html><a href="https://2011.igem.org/Team:British_Columbia/Characterized">Characterization details here!</a></html> | ||
| + | |||
==Modeling== | ==Modeling== | ||
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'''Monoterpene Production in Yeast''' | '''Monoterpene Production in Yeast''' | ||
| - | + | Our goal is to develop a model to assay the secretion and production of monoterpenes in yeast. | |
'''Mountain Pine Beetle Epidemic''' | '''Mountain Pine Beetle Epidemic''' | ||
| + | Our goal is to construct a mathematical model that can predict the dynamics of the mountain pine beetle populations in B.C. under the influence of our synthetic product. This model can be coupled with the strategic planning for future conservation initiatives. | ||
| + | |||
| + | <html><a href="https://2011.igem.org/Team:British_Columbia/Modeling">Modeling details here!</a></html> | ||
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'''Outreach at Science World''' | '''Outreach at Science World''' | ||
| + | |||
| + | <html><a href="https://2011.igem.org/Team:British_Columbia/Modeling">Outreach details here!</a></html> | ||
Revision as of 17:39, 13 August 2011
Wet Lab
Production of Monoterpenes in Yeast
From an industrial perspective, this yeast will be cultured and harvested for monoterpenes, which will be analysed by gas chromatography mass spectrometry (GC-MS). Yeast is a highly utilized organism in industry (e.g. breweries and also the recently established Amyris biotech company that uses yeast to produce anti-Malarial therapeutics amongst other valuable compounds). We envision that monoterpene-producing yeast will not only be industrially profitable, but also help to alleviate the unsustainable consumption of nature's resources in the quest for biofuels, pharmaceuticals, flavours/fragrances etc.
From an environmental perspective, this yeast will be co-cultured with the bluestain fungus to determine if monoterpene-producing yeast is able to inhibit the growth of the fungus. This yeast is also a biological system that can be screened for potential targets against the fungus. For instance, since the yeast is actively producing monoterpenes, it is possible to look for yeast that have particular mutations that cause increased intolerance against monoterpenes. These mutations will reveal potential strategies against the fungus.
Monoterpene Synthases
Our goal is to express synthases that produce monoterpenes (specifically limonene, pinene, carene and cineole) in yeast. Genes encoding these synthases will be inserted into yeast gateway vectors as well as the biobrick plasmid PSB1C3.
Metabolic Genes
Our goal is to express metabolic enzymes (specifically, erg20-2, Idi1 and Hmg2) that optimize the mevalonate pathway in yeast for monoterpene production.
Characterization details here!
Modeling
Monoterpene Production in Yeast
Our goal is to develop a model to assay the secretion and production of monoterpenes in yeast.
Mountain Pine Beetle Epidemic
Our goal is to construct a mathematical model that can predict the dynamics of the mountain pine beetle populations in B.C. under the influence of our synthetic product. This model can be coupled with the strategic planning for future conservation initiatives.
Human Practices
Outreach at Science World
