Team:British Columbia/Notebook/Week 2

From 2011.igem.org

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(June 13 2011)
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==June 13 2011==
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'''3-Carene'''
[[File:Daisykissprare.jpg]]
[[File:Daisykissprare.jpg]]
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'''3-Carene'''
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Daisy is working with the 3-carene monoterpene synthase that contains three restriction sites that need to be removed: EcoRI, XbaI, PstI. She worked hard, SDMing away over the weekend and has now only one more site to modify. We learnt from Chris Keeling that the truncated version of the synthase will increase the efficiency of the enzyme...
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Daisy is working with the synthase 3-carene; 3-carene has three restriction sites that need to be modified (EcoRI, XbaI, PstI). She worked over the weekend, and has one more site to modify.
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'''In other news...'''
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We learned that the truncated version of the synthase will increase the efficiency of the enzyme.
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==June 15 2011==
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Canucks lost to the Bruins. It was a sad day for all...
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==June 18 2011==
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'''3-Carene'''
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Daisy did a multiple site-directed mutagenesis using three sets of forward and reverse primers which contain the desired changes.
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The Canucks lost to the Bruins. It was a sad day for all...

Revision as of 02:38, 4 August 2011

Team: British Columbia - 2011.igem.org