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Team:British Columbia/Notebook/19 July 2011
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*Despite not getting any sequencing results, the restriction digest gels clearly showed that there were no cut sites in the synthase, so Jacob decided to try getting the synthases on biobricks. Today, Jacob ran a biobrick PCR to add the proper prefix and suffix to his synthase, but it did not work. | *Despite not getting any sequencing results, the restriction digest gels clearly showed that there were no cut sites in the synthase, so Jacob decided to try getting the synthases on biobricks. Today, Jacob ran a biobrick PCR to add the proper prefix and suffix to his synthase, but it did not work. | ||
<b>Miscellanea</b> | <b>Miscellanea</b> | ||
*New ampicillin plates were made, and the pag 413 GPD plasmid (for putting our synthases in yeast) was amplified. After the plasmid was amplified, the team decided to use another plasmid, making the amplified pag 413 GPD plasmid irrelevant for the project. | *New ampicillin plates were made, and the pag 413 GPD plasmid (for putting our synthases in yeast) was amplified. After the plasmid was amplified, the team decided to use another plasmid, making the amplified pag 413 GPD plasmid irrelevant for the project. | ||
Latest revision as of 00:28, 31 July 2011
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July 19 2011
1,8-cineole
- Despite not getting any sequencing results, the restriction digest gels clearly showed that there were no cut sites in the synthase, so Jacob decided to try getting the synthases on biobricks. Today, Jacob ran a biobrick PCR to add the proper prefix and suffix to his synthase, but it did not work.
Miscellanea
- New ampicillin plates were made, and the pag 413 GPD plasmid (for putting our synthases in yeast) was amplified. After the plasmid was amplified, the team decided to use another plasmid, making the amplified pag 413 GPD plasmid irrelevant for the project.
