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Team:British Columbia/Notebook/29 June 2011
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*Verified yesterday's SDM-PCR products through gel electrophoresis. No bands were present in any of the lanes. | *Verified yesterday's SDM-PCR products through gel electrophoresis. No bands were present in any of the lanes. | ||
*Troubleshooting: The dNTP could have been too old/contaminated (we had troubles with this particular tube of dNTP from last year); the concentrations for the reagents used were not optimized - Marianne researched protocols and optimized the SDM-PCR protocol. We (Vicki and Marianne) will test it tomorrow. | *Troubleshooting: The dNTP could have been too old/contaminated (we had troubles with this particular tube of dNTP from last year); the concentrations for the reagents used were not optimized - Marianne researched protocols and optimized the SDM-PCR protocol. We (Vicki and Marianne) will test it tomorrow. | ||
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| + | ====1,8-Cineole==== | ||
| + | Jacob transformed competent cells with his SDM-PCR product. | ||
| + | Ran SDM-PCR on variant of original synthase. | ||
Revision as of 20:17, 4 July 2011
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June 29 2011
Training: The plan was to make kanamycin plates, but this was at a stand-still as we had troubles locating the tube of kanamycin sulfate salt.
beta-pinene & (-)-limonene synthase
- Verified yesterday's SDM-PCR products through gel electrophoresis. No bands were present in any of the lanes.
- Troubleshooting: The dNTP could have been too old/contaminated (we had troubles with this particular tube of dNTP from last year); the concentrations for the reagents used were not optimized - Marianne researched protocols and optimized the SDM-PCR protocol. We (Vicki and Marianne) will test it tomorrow.
1,8-Cineole
Jacob transformed competent cells with his SDM-PCR product. Ran SDM-PCR on variant of original synthase.
