Team:SJTU-BioX-Shanghai

From 2011.igem.org

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<div id="cnt"><h2>Countdown</h2><embed src="http://www.oneplusyou.com/bb/files/countdown/countdown.swf?co=0066ff&bgcolor=eeeeee&date_month=10&date_day=15&date_year=2011&un=iGEM World Jamboree 2011&size=normal&mo=11&da=5&yr=2011" type="application/x-shockwave-flash" pluginspage="http://www.macromedia.com/go/getflashplayer" width="200 px" height="60 px" wmode="transparent"></embed></div>
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<div id="vst"><h2>Visitors</h2><a href="http://www2.clustrmaps.com/user/8c3de420"><img src="http://www2.clustrmaps.com/stats/maps-no_clusters/2011.igem.org-Team-SJTU-BioX-Shanghai-thumb.jpg" alt="Locations of visitors to this page" /></a></div>
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<a href="http://www.merckmillipore.com/millipore"><img src="/wiki/images/e/ec/11SJTU_merck.jpg" alt="Merck millipore" height="80 px" /></a><h5>Merck Millipore</h5></div>
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<a href="http://www.sjtu.edu.cn"><img src="/wiki/images/7/70/11SJTU-SJTU-logo.jpg" alt="Shanghai Jiao Tong University" height="50 px" margin="auto" /></a><h5>Shanghai Jiao Tong University</h5></div>
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<a href="http://www.bio-x.cn"><img src="/wiki/images/8/81/11SJTU_bio-x.jpg" alt="Bio-X Institutes" height="50px"/></a><h5>Bio-X Institutes</h5></div>
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<a href="http://www.genscript.com/"><img src="/wiki/images/7/7c/11SJTU-Genscript.jpg" alt="GenScript" height="50px"/></a><h5>GenScript</h5></div>
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    <a href="/Team:SJTU-BioX-Shanghai"><li><b>Home</b></li></a>
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  <li><a href="/Team:SJTU-BioX-Shanghai/Project"><b>Project</b></a>
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        <div><ul class="menu">
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            <a href="/Team:SJTU-BioX-Shanghai/Project"><li>Overview</li></a>
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            <li><a href="/Team:SJTU-BioX-Shanghai/Project/Subproject1" >Rare-Codon Switch</a>
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                  <a href="/Team:SJTU-BioX-Shanghai/Project/Subproject1" ><li>Overview</li></a>
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                  <a href="/Team:SJTU-BioX-Shanghai/Project/Subproject1-1" ><li>Rare tRNA amount</li></a>
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                  <a href="/Team:SJTU-BioX-Shanghai/Project/Subproject1-2" ><li>aaRS</li></a>
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                  <a href="/Team:SJTU-BioX-Shanghai/Project/Subproject1-3" ><li>Rare codon</li></a>
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                  <a href="/Team:SJTU-BioX-Shanghai/Project/Subproject1/Modeling" ><li>Modeling</li></a>
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              </ul></div></li>
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            <a href="/Team:SJTU-BioX-Shanghai/Project/Subproject2" ><li>Stop-Codon Switch</li></a>
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            <a href="/Team:SJTU-BioX-Shanghai/Project/Subproject3" ><li>Initial-Codon Switch</li></a>
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            <li><a>Application</a>
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                  <a href="/Team:SJTU-BioX-Shanghai/Project/Application-1" ><li>Regulating tools</li></a>
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                  <a href="/Team:SJTU-BioX-Shanghai/Project/Application-2" ><li style="height:36px"> Methods for protein function research
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                  <a href="/Team:SJTU-BioX-Shanghai/Project/Application-3" ><li style="height:36px">Modified aaRS Library
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    <a href="/Team:SJTU-BioX-Shanghai/Parts/Data_Page"><li><b>Data Page</b></li></a>
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    <a href="/Team:SJTU-BioX-Shanghai/Parts"> <li><b>Parts</b>  </li></a>               
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    <li><a href="/Team:SJTU-BioX-Shanghai/Team"><b>Team</b></a>
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        <div><ul class="menu">
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            <a href="/Team:SJTU-BioX-Shanghai/Team"><li>Overview</li></a>
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            <a href="/Team:SJTU-BioX-Shanghai/Team/Members"><li>Members</li></a>
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            <a href="/Team:SJTU-BioX-Shanghai/Team/Gallery"><li>Gallery</li></a>
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        </ul></div>
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      </li>
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    <li><b>Notebook</b>
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            <a href="/Team:SJTU-BioX-Shanghai/Notebook/Lablog"><li>Lablog</li></a>
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            <a href="/Team:SJTU-BioX-Shanghai/Notebook/Protocol"><li>Protocol</li></a>
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    <li><b>Consideration</b>
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    <a href="/Team:SJTU-BioX-Shanghai/Human_practice"> <li>Human Practice </li></a>
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    <a href="/Team:SJTU-BioX-Shanghai/Safety"><li>Safety</li></a>   
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    <a href="/Team:SJTU-BioX-Shanghai/Attributions"> <li>Attributions</li></a>
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Revision as of 18:08, 28 October 2011

    •

    Countdown

    Visitors

    Locations of visitors to this page

    Sponsors

    Merck millipore
    Merck Millipore
    Shanghai Jiao Tong University
    Shanghai Jiao Tong University
    Bio-X Institutes
    Bio-X Institutes
    GenScript
    GenScript
    The Project: Codon Switch Controlling Protein Biosynthesis

    SJTU-BioX-Shanghai iGEM team is designing a set of Codon-Switches that regulate target protein biosynthesis (translation).

    In our Rare-Codon Switch, the translation of the protein can be finely turned up/down with the control of rare tRNA amount, aaRS that charges the rare tRNA and rare codons.

    Besides, our device can be made into switches that can be turned on/off without background noise in two ways. One is to use stop codon as the controlling element, the Stop-Codon Switch. The other is to use any codon but the original start codon to initiate translation, the Initial-Codon Switch.

    Project

    Our design has expanded the regulating tools for synthetic biology and introduced brand-new methods for protein function analysis. See more about project applications, click here.

    Team
    Parts
    Human practice
    Achievements

    right Developed tRNA and aminoacyl tRNA synthetase (aaRS) from the natural biological system into the Modulator, a controlling device for protein biosynthesis. The function of this device has been fully characterized. Brand-new features of the device are also discovered.

    right Constructed 4 Modulators (including 8 biobricks) and 13 Reporter biobricks in our 3 subprojedts to test our controlling system. Create parts, including our favorite parts have been sent to Registry.

    right Modified pre-existing reporter genes luciferase and RFP and created a series of new reporter genes. These reporters have been excellent devices in characterizing our system.

    right Built our Rare-Codon Switch in silic and demonstrated the reliability of the modelthrough our experiment results. The data in silic and data experimental matches well.

    right Analyzed the structure of 18 aaRS with structural biological methods. Designed structures of 14 modified aaRS deprived of their anticodon recognition ability. This work can be used to expand our device aaRS Modulator.

    right Designed two different models for how our device can be applied to controlling metabolic networks, which expands the regulating tools for synthetic biology.

    right Developed two brand-new ways that facilitate the study of protein structure. One for incorporating point mutation into protein. The other for producing truncated proteins to help study the important protein domains.

    right Design our Human Practice Project: T-PostCards to promote our views on synthetic biology through communications and connections.

    right Attended the 2011 China Meet Up and presented our project idea to other iGEMer and advisors.

    Retrieved from "http://2011.igem.org/Team:SJTU-BioX-Shanghai"