Team:Osaka/week7

From 2011.igem.org

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{{Osaka}}
{{Osaka}}
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__NOTOC__
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==September 11 (Sun)==
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==September 11(Sun)==
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# Gel extraction of digests
# Gel extraction of digests
# Miniprep of yesterday's culture
# Miniprep of yesterday's culture
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==September 12(Mon)==
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==September 12 (Mon)==
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# To liquid culture:103,104,105,106
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==September 13 (Tue)==
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# Miniprep of yesterday's culture
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# Restriction digests of minipreppped parts
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# Gel electrophoresis of digests
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# Ligation
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#* 107(A):12(upstream)+103(downstream)+1-1G(vector)
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#* 108(A):12(upstream)+104(downstream)+1-1G(vector)
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# Transformation of ligation products
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==September 13(Tue)==
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==September 14 (Wed)==
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# To liquid culture:107,108
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==September 15 (Thu)==
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# Miniprep of yesterday's culture
 +
# Restriction digests of minipreppped parts
 +
# Gel electrophoresis of digests
 +
# To liquid culture:108
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==September 14(Wed)==
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==September 16 (Fri)==
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# Miniprep of yesterday's culture
 +
# Restriction digests of minipreppped parts
 +
# Gel electrophoresis of digests
-
==September 15(Thu)==
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==September 17 (Sat)==
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# Ligation
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#* 109(K):1-2M(upstream)+101(downstream)+1-5A(vector)
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#* 110(K):1-2M(upstream)+102(downstream)+1-5A(vector)
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#* 111(K):1-2M(upstream)+103(downstream)+1-5A(vector)
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#* 112(K):1-2M(upstream)+104(downstream)+1-5A(vector)
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# Transformation of ligation products
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==September 16(Fri)==
 
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==September 17(Sat)==
 
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[[Team:Osaka/Notebook|Back to Notebook]]
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week [[Team:Osaka/week1|1]]|[[Team:Osaka/week2|2]]|[[Team:Osaka/week3|3]]|[[Team:Osaka/week4|4]]|[[Team:Osaka/week5|5]]|[[Team:Osaka/week6|6]]|'''7'''|[[Team:Osaka/week8|8]]|[[Team:Osaka/week9|9]]|[[Team:Osaka/week10|10]]|[[Team:Osaka/week11|11]]|[[Team:Osaka/week12|12]]|[[Team:Osaka/week13|13]]
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[[Team:Osaka/Notebook|Back to Notebook]]
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Latest revision as of 05:51, 19 October 2011

September 11 (Sun)

  1. Gel extraction of digests
  2. Miniprep of yesterday's culture
  3. Restriction digests of minipreppped parts
  4. Gel electrophoresis of digests
  5. Ligation
    • 103(C):PprM+1-3A(vector)
    • 104(C):RecA+1-3A(vector)
    • 105(A):12(upstream)+101(downstream)+1-1G(vector)
    • 106(A):12(upstream)+102(downstream)+1-1G(vector)
  6. Transformation of ligation products


September 12 (Mon)

  1. To liquid culture:103,104,105,106

September 13 (Tue)

  1. Miniprep of yesterday's culture
  2. Restriction digests of minipreppped parts
  3. Gel electrophoresis of digests
  4. Ligation
    • 107(A):12(upstream)+103(downstream)+1-1G(vector)
    • 108(A):12(upstream)+104(downstream)+1-1G(vector)
  5. Transformation of ligation products

September 14 (Wed)

  1. To liquid culture:107,108

September 15 (Thu)

  1. Miniprep of yesterday's culture
  2. Restriction digests of minipreppped parts
  3. Gel electrophoresis of digests
  4. To liquid culture:108

September 16 (Fri)

  1. Miniprep of yesterday's culture
  2. Restriction digests of minipreppped parts
  3. Gel electrophoresis of digests


September 17 (Sat)

  1. Ligation
    • 109(K):1-2M(upstream)+101(downstream)+1-5A(vector)
    • 110(K):1-2M(upstream)+102(downstream)+1-5A(vector)
    • 111(K):1-2M(upstream)+103(downstream)+1-5A(vector)
    • 112(K):1-2M(upstream)+104(downstream)+1-5A(vector)
  2. Transformation of ligation products


Back to Notebook

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Retrieved from "http://2011.igem.org/Team:Osaka/week7"