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Template:Protocols
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<a href="/Team:British_Columbia/Protocols/Peace"><b>iGEM Tips for Success</a><br></b> | <a href="/Team:British_Columbia/Protocols/Peace"><b>iGEM Tips for Success</a><br></b> | ||
| - | Things to think about when designing your project and experiments, as well as general safety rules.<br> | + | Things to think about when designing your project and experiments, as well as general safety rules.<br><br> |
<b><a href="/Team:British_Columbia/Protocols/Sdm">Site Directed Mutagenesis</a><br></b> | <b><a href="/Team:British_Columbia/Protocols/Sdm">Site Directed Mutagenesis</a><br></b> | ||
| - | A molecular biology technique in which a mutation is created at a defined site in a DNA molecule.<br> | + | A molecular biology technique in which a mutation is created at a defined site in a DNA molecule.<br><br> |
<b><a href="/Team:British_Columbia/Protocols/Bacteria">Bacterial Standard Operating Protocols</a><br></b> | <b><a href="/Team:British_Columbia/Protocols/Bacteria">Bacterial Standard Operating Protocols</a><br></b> | ||
| - | How to prepare competent cells, transform your construct into competent cells, and express your protein from a lac promoter.<br> | + | How to prepare competent cells, transform your construct into competent cells, and express your protein from a lac promoter.<br><br> |
<b><a href="/Team:British_Columbia/Protocols/Yeast">Yeast Standard Operating Protocols</a><br></b> | <b><a href="/Team:British_Columbia/Protocols/Yeast">Yeast Standard Operating Protocols</a><br></b> | ||
| - | How to transform your construct into yeast, obtain crude extract for SDS-PAGE, and perform GFP fixation for microscopy and fluorescence-activated cell sorting.<br> | + | How to transform your construct into yeast, obtain crude extract for SDS-PAGE, and perform GFP fixation for microscopy and fluorescence-activated cell sorting.<br><br> |
<b><a href="/Team:British_Columbia/Protocols/Gcms">Gas Chromatography-Mass Spectrometry (GC-MS)</a><br></b> | <b><a href="/Team:British_Columbia/Protocols/Gcms">Gas Chromatography-Mass Spectrometry (GC-MS)</a><br></b> | ||
| - | A method that combines the features of gas-liquid chromatography and mass spectrometry to identify different substances within a test sample.<br> | + | A method that combines the features of gas-liquid chromatography and mass spectrometry to identify different substances within a test sample.<br><br> |
| - | <b><a href="/Team:British_Columbia/Protocols/ | + | <b><a href="/Team:British_Columbia/Protocols/Beetle">Beetle Transfer Experiments</a><br></b> |
| - | Preliminary experiments to probe the efficiency of transferring yeast via beetle vector. | + | Preliminary experiments to probe the efficiency of transferring yeast via beetle vector.<br><br> |
| - | </b> | + | <b><a href="/Team:British_Columbia/Protocols/Fungi">Yeast-Fungi Co-culture Experiments</a><br></b> |
| - | </html> | + | Preliminary experiments to investigate the competitive interactions between yeast and the Bluestain Fungus. |
| + | </br></br></br> | ||
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Latest revision as of 17:46, 18 October 2011
iGEM Tips for Success
Things to think about when designing your project and experiments, as well as general safety rules.
Site Directed Mutagenesis
A molecular biology technique in which a mutation is created at a defined site in a DNA molecule.
Bacterial Standard Operating Protocols
How to prepare competent cells, transform your construct into competent cells, and express your protein from a lac promoter.
Yeast Standard Operating Protocols
How to transform your construct into yeast, obtain crude extract for SDS-PAGE, and perform GFP fixation for microscopy and fluorescence-activated cell sorting.
Gas Chromatography-Mass Spectrometry (GC-MS)
A method that combines the features of gas-liquid chromatography and mass spectrometry to identify different substances within a test sample.
Beetle Transfer Experiments
Preliminary experiments to probe the efficiency of transferring yeast via beetle vector.
Yeast-Fungi Co-culture Experiments
Preliminary experiments to investigate the competitive interactions between yeast and the Bluestain Fungus.
Things to think about when designing your project and experiments, as well as general safety rules.
Site Directed Mutagenesis
A molecular biology technique in which a mutation is created at a defined site in a DNA molecule.
Bacterial Standard Operating Protocols
How to prepare competent cells, transform your construct into competent cells, and express your protein from a lac promoter.
Yeast Standard Operating Protocols
How to transform your construct into yeast, obtain crude extract for SDS-PAGE, and perform GFP fixation for microscopy and fluorescence-activated cell sorting.
Gas Chromatography-Mass Spectrometry (GC-MS)
A method that combines the features of gas-liquid chromatography and mass spectrometry to identify different substances within a test sample.
Beetle Transfer Experiments
Preliminary experiments to probe the efficiency of transferring yeast via beetle vector.
Yeast-Fungi Co-culture Experiments
Preliminary experiments to investigate the competitive interactions between yeast and the Bluestain Fungus.
