Team:SJTU-BioX-Shanghai/Project/Subproject1/Results

From 2011.igem.org

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'''Action:''' when rare tRNA<sup>Arg</sup> is over expressed, the amount of tRNA<sup>Arg</sup> in the tRNA pool is largely increased, which facilitates ribosome to get through the tandem of AGG codons in the RFP's mRNA.
'''Action:''' when rare tRNA<sup>Arg</sup> is over expressed, the amount of tRNA<sup>Arg</sup> in the tRNA pool is largely increased, which facilitates ribosome to get through the tandem of AGG codons in the RFP's mRNA.
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*[[Team:SJTU-BioX-Shanghai/Project/Subproject1/Results_2|'''tRNA Modulator + Reporter for Quantitative Analysis ''']]
+
[[Team:SJTU-BioX-Shanghai/Project/Subproject1/Results_2|'''tRNA Modulator + Reporter for Quantitative Analysis ''']]
 +
 
'''Action:''' when rare tRNA<sup>Arg</sup> is over expressed, the amount of tRNA<sup>Arg</sup> in the tRNA pool is largely increased, which facilitates ribosome to get through the tandem of AGG codons in the modified luciferase's mRNA.
'''Action:''' when rare tRNA<sup>Arg</sup> is over expressed, the amount of tRNA<sup>Arg</sup> in the tRNA pool is largely increased, which facilitates ribosome to get through the tandem of AGG codons in the modified luciferase's mRNA.
-
*'''Results Conclusion'''
+
*'''Conclusion'''
 +
We have analyzed the three potential factors influencing Rare-Codon Switch and have fully characterized our device through working curves, demonstrating the function of this device as a regulating tool for protein biosynthesis. We have also identified influential factors other than the three proposed above.
----
----
===aaRS Modulator System===
===aaRS Modulator System===
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*[[Team:SJTU-BioX-Shanghai/Project/Subproject1/Results_3|'''aaRS Modulator + Reporter for Qualitative Analysis ''']]
+
*'''System Analysis'''
 +
----
 +
[[Team:SJTU-BioX-Shanghai/Project/Subproject1/Results_3|'''aaRS Modulator + Reporter for Qualitative Analysis ''']]
'''Action:''' When TDRS is expressed under IPTG induction, it can charge tRNA<sup>Asp</sup>-AGG with Asp. tRNA<sup>Asp</sup>-AGG then recognizes the rare codon AGG on RFP's mRNA and add Asp to the polypeptide chain. RFP is expressed.
'''Action:''' When TDRS is expressed under IPTG induction, it can charge tRNA<sup>Asp</sup>-AGG with Asp. tRNA<sup>Asp</sup>-AGG then recognizes the rare codon AGG on RFP's mRNA and add Asp to the polypeptide chain. RFP is expressed.
 +
[[Team:SJTU-BioX-Shanghai/Project/Subproject1/Results_4|'''aaRS Modulator + Reporter for Quantitative analysis''']]
 +
'''Action:''' When TDRS is expressed under IPTG induction, it can charge tRNA<sup>Asp</sup>-AGG with Asp. tRNA<sup>Asp</sup>-AGG then recognizes the rare codon AGG on the modified luciferase's mRNA and add Asp to the polypeptide chain. Modified luciferase is expressed.
-
*[[Team:SJTU-BioX-Shanghai/Project/Subproject1/Results_4|'''aaRS Modulator + Reporter for Quantitative analysis''']]
+
*'''Conclusion'''
 +
We have designed modified AspRS and tested its aminoacylation ability, ensuring the function of our Rare-Codon Switch.
-
'''Action:''' When TDRS is expressed under IPTG induction, it can charge tRNA<sup>Asp</sup>-AGG with Asp. tRNA<sup>Asp</sup>-AGG then recognizes the rare codon AGG on the modified luciferase's mRNA and add Asp to the polypeptide chain. Modified luciferase is expressed.
+
----

Latest revision as of 02:37, 6 October 2011

    •



    Rare-Codon Switch

    Results

    Action: Combinations of Modulators and Reporters

    Select a Modulator and a Reporter, then click 11SJTU arrow.jpg to see results!


    tRNA Modulator System

    • System Analysis

    tRNA Modulator + Reporter for Qualitative Analysis

    Action: when rare tRNAArg is over expressed, the amount of tRNAArg in the tRNA pool is largely increased, which facilitates ribosome to get through the tandem of AGG codons in the RFP's mRNA.

    tRNA Modulator + Reporter for Quantitative Analysis

    Action: when rare tRNAArg is over expressed, the amount of tRNAArg in the tRNA pool is largely increased, which facilitates ribosome to get through the tandem of AGG codons in the modified luciferase's mRNA.

    • Conclusion

    We have analyzed the three potential factors influencing Rare-Codon Switch and have fully characterized our device through working curves, demonstrating the function of this device as a regulating tool for protein biosynthesis. We have also identified influential factors other than the three proposed above.

    aaRS Modulator System

    • System Analysis

    aaRS Modulator + Reporter for Qualitative Analysis

    Action: When TDRS is expressed under IPTG induction, it can charge tRNAAsp-AGG with Asp. tRNAAsp-AGG then recognizes the rare codon AGG on RFP's mRNA and add Asp to the polypeptide chain. RFP is expressed.

    aaRS Modulator + Reporter for Quantitative analysis

    Action: When TDRS is expressed under IPTG induction, it can charge tRNAAsp-AGG with Asp. tRNAAsp-AGG then recognizes the rare codon AGG on the modified luciferase's mRNA and add Asp to the polypeptide chain. Modified luciferase is expressed.

    • Conclusion

    We have designed modified AspRS and tested its aminoacylation ability, ensuring the function of our Rare-Codon Switch.

    Retrieved from "http://2011.igem.org/Team:SJTU-BioX-Shanghai/Project/Subproject1/Results"