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Team:SJTU-BioX-Shanghai/Project/Subproject1/Results 1
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===Results=== | ===Results=== | ||
| - | We have designed PT7-RFP-6AGG (BBa_K567017) for qualitative analysis of tRNA modulator. The function of tRNA Modulator is tested by observing red fluorescence in the expression system. | + | We have designed PT7-RFP-6AGG ([http://partsregistry.org/wiki/index.php?title=Part:BBa_K567017 BBa_K567017]) for qualitative analysis of tRNA modulator. The function of tRNA Modulator is tested by observing red fluorescence in the expression system. |
| - | In the experiment group, ER2566 is co-transformed with both Modulator ''LacI''-Ptrc-tRNA<sup>Arg</sup> and Reporter PT7-RFP-6AGG (BBa_K567017). When Modulator is induced, tRNA<sup>Arg</sup> is overexpressed, ribosome can get through the 6 consecutive AGG codons, RFP can be successfully expressed. For the control group in which only Reporter is transformed, RFP expresssion is hindered. The test results are as follows. | + | In the experiment group, ER2566 is co-transformed with both Modulator ''LacI''-Ptrc-tRNA<sup>Arg</sup> and Reporter PT7-RFP-6AGG ([http://partsregistry.org/wiki/index.php?title=Part:BBa_K567017 BBa_K567017]). When Modulator is induced, tRNA<sup>Arg</sup> is overexpressed, ribosome can get through the 6 consecutive AGG codons, RFP can be successfully expressed. For the control group in which only Reporter is transformed, RFP expresssion is hindered. The test results are as follows. |
[[image:11SJTU-tRNA_Modulator%2BRFP-confocal.jpg|frame|center|''fig 1.'' Confocal Microscope examining function of tRNA Modulator + Reporter for Qualitative Analysis. ]] | [[image:11SJTU-tRNA_Modulator%2BRFP-confocal.jpg|frame|center|''fig 1.'' Confocal Microscope examining function of tRNA Modulator + Reporter for Qualitative Analysis. ]] | ||
Revision as of 20:40, 5 October 2011
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Rare-Codon Switch tRNA Modulator + Reporter for Qualitative Analysis
ResultsWe have designed PT7-RFP-6AGG ([http://partsregistry.org/wiki/index.php?title=Part:BBa_K567017 BBa_K567017]) for qualitative analysis of tRNA modulator. The function of tRNA Modulator is tested by observing red fluorescence in the expression system. In the experiment group, ER2566 is co-transformed with both Modulator LacI-Ptrc-tRNAArg and Reporter PT7-RFP-6AGG ([http://partsregistry.org/wiki/index.php?title=Part:BBa_K567017 BBa_K567017]). When Modulator is induced, tRNAArg is overexpressed, ribosome can get through the 6 consecutive AGG codons, RFP can be successfully expressed. For the control group in which only Reporter is transformed, RFP expresssion is hindered. The test results are as follows.  fig 1. Confocal Microscope examining function of tRNA Modulator + Reporter for Qualitative Analysis. Confocal Microscope analysis showed that RFP expression is hindered by the six consecutive AGG codons. After tRNAArg expresson is induced, RFP-6AGG expression is ensured.
 fig 2. tRNA Modulator + Reporter for Qualitative Analysis. tRNA Modulator + RFP-6AGG(the middle three) emit bright red fluorescence as wild type RFP(first one from the left). Control(first one from the right) exhibits no red fluoresence.
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