Team:SJTU-BioX-Shanghai/Project/Subproject1/Results

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'''Action:''' when rare tRNA<sup>Arg</sup> is over expressed, the amount of tRNA<sup>Arg</sup> in the tRNA pool is largely increased, which facilitates ribosome to get through the tandem of AGG codons in the RFP's mRNA.
'''Action:''' when rare tRNA<sup>Arg</sup> is over expressed, the amount of tRNA<sup>Arg</sup> in the tRNA pool is largely increased, which facilitates ribosome to get through the tandem of AGG codons in the RFP's mRNA.
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Revision as of 16:37, 5 October 2011

    •



    Rare-Codon Switch

    Results

    Action: Combinations of Modulators and Reporters

    Select a Modulator and a Reporter, then click 11SJTU arrow.jpg to see results!


    tRNA Modulator System

    • System Analysis

    Action: when rare tRNAArg is over expressed, the amount of tRNAArg in the tRNA pool is largely increased, which facilitates ribosome to get through the tandem of AGG codons in the RFP's mRNA.


    Action: when rare tRNAArg is over expressed, the amount of tRNAArg in the tRNA pool is largely increased, which facilitates ribosome to get through the tandem of AGG codons in the modified luciferase's mRNA.

    aaRS Modulator System

    Action: When TDRS is expressed under IPTG induction, it can charge tRNAAsp-AGG with Asp. tRNAAsp-AGG then recognizes the rare codon AGG on RFP's mRNA and add Asp to the polypeptide chain. RFP is expressed.


    Action: When TDRS is expressed under IPTG induction, it can charge tRNAAsp-AGG with Asp. tRNAAsp-AGG then recognizes the rare codon AGG on the modified luciferase's mRNA and add Asp to the polypeptide chain. Modified luciferase is expressed.

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