Team:UNIST Korea

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<font color="white"><br/>Genetically Modified Organisms (GMOs) pose a significant threat to human health and the environment. Despite being a promising candidate in various processes such as production of drugs and bio-commodities, GMOs do not experience a warm welcome from the human society. Fear of destruction of the natural biota
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suppresses all of the benefits offered by GMOs. Horizontal gene transfer (HGT) from GMOs to some pathogenic microbes, which leads to superbugs, is yet another major concern with GMOs. Here, we design a GMO with a built-in triggered-suicide system that is inducible by environmental signals (such as light, oxygen, heavy metals or quorum sensing system). This selfless GMO would kill itself on receiving environmental cues when it is released from its native completely closed system (fermentor or laboratory) to the open system (surrounding environment). We propose the use of a novel suicide platform using the genes coding for the restriction enzyme, DpnI that would chew up all methylated DNAs. This novel suicide platform may help prevent HGT and in turn enhance the potential benefits offered by GMOs.''<br /></font>
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<font face=calibri><font size="9"><font color="cc0099"><center>CHOp-Coli-LATE</p></font></font>
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<br/><br/>
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<font size="6"><font color=cc0066><center>Synthetic Microbes</p></font></font><br/>
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<p align="justify"><font size=4 color=maroon>Synthetic Microbes are gaining increased importance in 21 st century. Synthetic Microbes are more promising hosts for fuel production, bioremediation and many other potential processes. There is an increased dependence on the synthetic microbes even for certain basic needs such as medicine and fuels. However, synthetic microbes do not experience a warm welcome from the general public because they are believed to endanger the environment.Synthetic Microbes: <br/><br/></P>
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<font size="5"><font color="Red">C</font></font>an escape from its restricted area and can
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<br/>  <font size="5"><font color="Red">H</font></font>orizontally tranfer its genetic materials to pathogens leading to super bugs that can go <br/>
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<font size="5"><font color="Red">O</font></font>ut of control of human and<br/>
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<font size="5"><font color="Red">C</font></font>ause epidemics; can <br/>
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<font size="5"><font color="Red">O</font></font>bdurately occupy the universe and can be a<br/>
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<font size="5"><font color="Red">L</font></font>ethal biological weapon hence synthetic microbes are being <br/>
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<font size="5"><font color="Red">A</font></font>bjured by the world as they<br/>
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<font size="5"><font color="Red">T</font></font>hreaten the existing biodiversity and are believed to be <br/>
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<font size="5"><font color="Red">E</font></font>nvironmentally damaging                        <font size="5"><font color="Red">ELYZIANS</font></font> <br/><br/></p><br/>
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<font size="6"><font color="cc0066">DNA is two sides of the same coin</font></font><br/><br/>
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<font size="4" color=maroon>
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<p align="justify">All advances in synthetic biology is because of the tiny double helical molecule called  <font size="5"><font color="Red">DNA</font></font>. All threats of synthetic biology is again because of these tiny molecules called <font size="5"><font color="Red">DNA</font></font>. Thus, DNA forms two sides of the same coin. <br /></font><br/><br/></font></p>
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<font size="6"><font color=cc0066> Overview of CHOCOLATE</font></font><br/><br/>
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<p style="float:right"><img src="https://static.igem.org/mediawiki/2011/a/a3/Skl32.jpg" height="290" width="250"></p><p align="justify">CHOCOLATE project aims at eradicating the negative aspects of synthetic biology by exploiting the defence mechanism used by natural microbes to differentiate its genome from that of the foreign DNA. Natural microbes utilize a group of restriction enzymes like dam, dcm and dpn to diferentiate its DNA from that of the foreign DNA. We simply modified the host defence machanism such that the synthetic microbe would identify its genome as its native when it stays in its desired environment and the host defence will be switched OFF. However, when the synthetic microbe senses that it is in a non-native environment it would turn ON its host defence to destroy its own DNA and kill itself. Thus, this system would not only favor cell death but also ensure that all the genetic materials are destroyed and guarantee that there would be no horizontal gene transfer. </font></font></img>
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Latest revision as of 14:34, 5 October 2011


CHOp-Coli-LATE

Synthetic Microbes


Synthetic Microbes are gaining increased importance in 21 st century. Synthetic Microbes are more promising hosts for fuel production, bioremediation and many other potential processes. There is an increased dependence on the synthetic microbes even for certain basic needs such as medicine and fuels. However, synthetic microbes do not experience a warm welcome from the general public because they are believed to endanger the environment.Synthetic Microbes:

Can escape from its restricted area and can
Horizontally tranfer its genetic materials to pathogens leading to super bugs that can go
Out of control of human and
Cause epidemics; can
Obdurately occupy the universe and can be a
Lethal biological weapon hence synthetic microbes are being
Abjured by the world as they
Threaten the existing biodiversity and are believed to be
Environmentally damaging ELYZIANS


DNA is two sides of the same coin

All advances in synthetic biology is because of the tiny double helical molecule called DNA. All threats of synthetic biology is again because of these tiny molecules called DNA. Thus, DNA forms two sides of the same coin.


Overview of CHOCOLATE

CHOCOLATE project aims at eradicating the negative aspects of synthetic biology by exploiting the defence mechanism used by natural microbes to differentiate its genome from that of the foreign DNA. Natural microbes utilize a group of restriction enzymes like dam, dcm and dpn to diferentiate its DNA from that of the foreign DNA. We simply modified the host defence machanism such that the synthetic microbe would identify its genome as its native when it stays in its desired environment and the host defence will be switched OFF. However, when the synthetic microbe senses that it is in a non-native environment it would turn ON its host defence to destroy its own DNA and kill itself. Thus, this system would not only favor cell death but also ensure that all the genetic materials are destroyed and guarantee that there would be no horizontal gene transfer.

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