Team:Calgary/Notebook/Protocols
From 2011.igem.org
(Difference between revisions)
Emily Hicks (Talk | contribs) |
|||
(4 intermediate revisions not shown) | |||
Line 4: | Line 4: | ||
TITLE=Protocols| | TITLE=Protocols| | ||
BODY=<html> | BODY=<html> | ||
- | <p>Here is a list of the procedures we used this summer. Each | + | <p>Here is a list of all the procedures we used this summer. Each contains a description and list of materials required.</p> |
<ul> | <ul> | ||
<h2>Microalgae Protocols</h2> | <h2>Microalgae Protocols</h2> | ||
<li><a href="https://2011.igem.org/Team:Calgary/Notebook/Protocols/Process5">Preparation of Algal f2 Media</a></li> | <li><a href="https://2011.igem.org/Team:Calgary/Notebook/Protocols/Process5">Preparation of Algal f2 Media</a></li> | ||
- | <li><a href="https://2011.igem.org/Team:Calgary/Notebook/Protocols/Process6">Glass Bead Algal | + | <li><a href="https://2011.igem.org/Team:Calgary/Notebook/Protocols/Process6">Glass Bead Algal Transformation</a></li> |
<li><a href="https://2011.igem.org/Team:Calgary/Notebook/Protocols/Process7">Chloroplast Isolation</a></li> | <li><a href="https://2011.igem.org/Team:Calgary/Notebook/Protocols/Process7">Chloroplast Isolation</a></li> | ||
- | <li><a href="https://2011.igem.org/Team:Calgary/Notebook/Protocols/Process8">Nuclear DNA | + | <li><a href="https://2011.igem.org/Team:Calgary/Notebook/Protocols/Process8">Nuclear DNA Extraction from Algae</a></li> |
<h2>Pseudomonas Biotinylation Techniques</h2> | <h2>Pseudomonas Biotinylation Techniques</h2> | ||
Line 17: | Line 17: | ||
<li><a href="https://2011.igem.org/Team:Calgary/Notebook/Protocols/Process24">Genomic DNA Isolation (from <i>Pseudomonas spp.</i>)</a></li> | <li><a href="https://2011.igem.org/Team:Calgary/Notebook/Protocols/Process24">Genomic DNA Isolation (from <i>Pseudomonas spp.</i>)</a></li> | ||
<li><a href="https://2011.igem.org/Team:Calgary/Notebook/Protocols/Process22">Biotinylation/Immunoprecipitation of Naphthenic Acids</a></li> | <li><a href="https://2011.igem.org/Team:Calgary/Notebook/Protocols/Process22">Biotinylation/Immunoprecipitation of Naphthenic Acids</a></li> | ||
- | <li><a href="https://2011.igem.org/Team:Calgary/Notebook/Protocols/Process23">Conjugation of <i>E. coli</i> plasmids to <i>Pseudomonas spp.</i> | + | <li><a href="https://2011.igem.org/Team:Calgary/Notebook/Protocols/Process23">Conjugation of <i>E. coli</i> plasmids to <i>Pseudomonas spp.</i></a></li> |
<h2>Electrochemical Techniques</h2> | <h2>Electrochemical Techniques</h2> | ||
Line 31: | Line 31: | ||
<li><a href="https://2011.igem.org/Team:Calgary/Notebook/Protocols/Process3">cDNA Synthesis</a></li> | <li><a href="https://2011.igem.org/Team:Calgary/Notebook/Protocols/Process3">cDNA Synthesis</a></li> | ||
<li><a href="https://2011.igem.org/Team:Calgary/Notebook/Protocols/Process4">Quantitative Real Time PCR</a></li> | <li><a href="https://2011.igem.org/Team:Calgary/Notebook/Protocols/Process4">Quantitative Real Time PCR</a></li> | ||
- | <li><a href="https://2011.igem.org/Team:Calgary/Notebook/Protocols/pBAD">Testing the pBAD- | + | <li><a href="https://2011.igem.org/Team:Calgary/Notebook/Protocols/pBAD">Testing the pBAD-<i>lacZ</i> Construct</a></li> |
<li><a href="https://2011.igem.org/Team:Calgary/Notebook/Protocols/Process9">Taq PCR Protocol</a></li> | <li><a href="https://2011.igem.org/Team:Calgary/Notebook/Protocols/Process9">Taq PCR Protocol</a></li> | ||
<li><a href="https://2011.igem.org/Team:Calgary/Notebook/Protocols/Process10">PCR Purification</a></li> | <li><a href="https://2011.igem.org/Team:Calgary/Notebook/Protocols/Process10">PCR Purification</a></li> | ||
<li><a href="https://2011.igem.org/Team:Calgary/Notebook/Protocols/Process11">Gel Extraction</a></li> | <li><a href="https://2011.igem.org/Team:Calgary/Notebook/Protocols/Process11">Gel Extraction</a></li> | ||
- | <li><a href="https://2011.igem.org/Team:Calgary/Notebook/Protocols/Process12">Testing the | + | <li><a href="https://2011.igem.org/Team:Calgary/Notebook/Protocols/Process12">Testing the <i>lacI-lacZ</i> I732901 Gene</a></li> |
<li><a href="https://2011.igem.org/Team:Calgary/Notebook/Protocols/Process13">Bacterial Transformation</a></li> | <li><a href="https://2011.igem.org/Team:Calgary/Notebook/Protocols/Process13">Bacterial Transformation</a></li> | ||
<li><a href="https://2011.igem.org/Team:Calgary/Notebook/Protocols/Process14">Rehydration of Registry DNA</a></li> | <li><a href="https://2011.igem.org/Team:Calgary/Notebook/Protocols/Process14">Rehydration of Registry DNA</a></li> | ||
Line 43: | Line 43: | ||
<li><a href="https://2011.igem.org/Team:Calgary/Notebook/Protocols/Process17">LB Agar Plates</a></li> | <li><a href="https://2011.igem.org/Team:Calgary/Notebook/Protocols/Process17">LB Agar Plates</a></li> | ||
<li><a href="https://2011.igem.org/Team:Calgary/Notebook/Protocols/Process18">Overnight Cultures</a></li> | <li><a href="https://2011.igem.org/Team:Calgary/Notebook/Protocols/Process18">Overnight Cultures</a></li> | ||
- | <li><a href="https://2011.igem.org/Team:Calgary/Notebook/Protocols/Process19">Preparing | + | <li><a href="https://2011.igem.org/Team:Calgary/Notebook/Protocols/Process19">Preparing Chemically Competent Cells (<i>E. coli</i>)</a></li> |
- | <li><a href="https://2011.igem.org/Team:Calgary/Notebook/Protocols/Process20">Preparing | + | <li><a href="https://2011.igem.org/Team:Calgary/Notebook/Protocols/Process20">Preparing Glycerol Stocks (<i>E. coli</i>) |
</ul> | </ul> |
Latest revision as of 03:53, 29 September 2011
![](https://static.igem.org/mediawiki/2011/c/c1/UCalgary_CircleHome.png)
![](https://static.igem.org/mediawiki/2011/d/d4/UCalgary_CircleTeam.png)
![](https://static.igem.org/mediawiki/2011/7/7d/UCalgary_CircleProject.png)
![](https://static.igem.org/mediawiki/2011/8/8d/UCalgary_CircleParts.png)
![](https://static.igem.org/mediawiki/2011/0/03/UCalgary_CircleNotebook.png)
![](https://static.igem.org/mediawiki/2011/8/84/UCalgary_CircleOutreach.png)
![](https://static.igem.org/mediawiki/2011/3/3c/UCalgary_CircleSponsors.png)
![](https://static.igem.org/mediawiki/2011/8/85/UCalgary_CircleiGEM.png)
![](https://static.igem.org/mediawiki/2011/4/42/UCalgary_TitleBar.png)
Protocols
![](https://static.igem.org/mediawiki/2011/e/ed/UCalgary_BodyBoxTop.png)
Here is a list of all the procedures we used this summer. Each contains a description and list of materials required.
- Preparation of Algal f2 Media
- Glass Bead Algal Transformation
- Chloroplast Isolation
- Nuclear DNA Extraction from Algae
- Plasmid Isolation (from Pseudomonas spp.)
- Genomic DNA Isolation (from Pseudomonas spp.)
- Biotinylation/Immunoprecipitation of Naphthenic Acids
- Conjugation of E. coli plasmids to Pseudomonas spp.
- Electrode Plating
- Buffer Preparation
- Electrical Settings
- Testing For Oxidation
- Plasmid Isolation (from E. coli)
- RNA Extraction
- cDNA Synthesis
- Quantitative Real Time PCR
- Testing the pBAD-lacZ Construct
- Taq PCR Protocol
- PCR Purification
- Gel Extraction
- Testing the lacI-lacZ I732901 Gene
- Bacterial Transformation
- Rehydration of Registry DNA
- Construction Techniques
- Agarose Gel Electrophresis
- LB Agar Plates
- Overnight Cultures
- Preparing Chemically Competent Cells (E. coli)
- Preparing Glycerol Stocks (E. coli)
Microalgae Protocols
Pseudomonas Biotinylation Techniques
Electrochemical Techniques
General Protocols
![](https://static.igem.org/mediawiki/2011/c/cb/UCalgary_BodyBoxBottom.png)